Discrimination of Helicobacter pullorum and Campylobacter lari by analysis of whole cell fatty acid extracts

Helicobacter pullorum and Campylobacter lari are rarely isolated from humans with acute enteritis. Hitherto the two species could only be identified by genotypic techniques. Gas liquid chromatography of whole cell fatty acid extracts is described as the first phenotypic method for discrimination of the two species. Cholesteryl glucoside, a characteristic feature of the genus Helicobacter, but seldom found in other bacteria, could not be detected in Helicobacter pullorum. Therefore, rapid determination of this glycolipid may serve as a discrimination marker for Helicobacter pullorum from most other Helicobacter specie

Discrimination of Helicobacter pullorum and Campylobacter lari by analysis of whole cell fatty acid extracts

Helicobacter pullorum and Campylobacter lari are rarely isolated from humans with acute enteritis. Hitherto the two species could only be identified by genotypic techniques. Gas liquid chromatography of whole cell fatty acid extracts is described as the first phenotypic method for discrimination of the two species. Cholesteryl glucoside, a characteristic feature of the genus Helicobacter, but seldom found in other bacteria, could not be detected in Helicobacter pullorum. Therefore, rapid determination of this glycolipid may serve as a discrimination marker for Helicobacter pullorum from most other Helicobacter species

Thermoelectric effects in a strongly correlated model for Nax_xCoO2_2

Thermal response functions of strongly correlated electron systems are of appreciable interest to the larger scientific community both theoretically and technologically. Here we focus on the infinitely correlated t-J model on a geometrically frustrated two-dimensional triangular lattice. Using exact diagonalization on a finite sized system we calculate the dynamical thermal response functions in order to determine the thermopower, Lorenz number, and dimensionless figure of merit. The dynamical thermal response functions is compared to the infinite frequency limit and shown to be very weak functions of frequency, hence, establishing the validity of the high frequency formalism recently proposed by Shastry for the thermopower, Lorenz number, and the dimensionless figure of merit. Further, the thermopower is demonstrated to have a low to mid temperature enhancement when the sign of the hopping parameter $t$ is switched from positive to negative for the geometrically frustrated lattice considered.Comment: 16 pages, 10 figures, color version available at http://physics.ucsc.edu/~peterson/mrpeterson-condmat-NCO.pdf. V.2 has fixed minor typos in Eq. 11, 19, 25, and 26. V.3 is a color versio

Double π0\pi^0 Photoproduction off the Proton at Threshold

The reaction $\gamma p \to \pi^0 \pi^0 p$ has been measured using the TAPS BaF$_2$ calorimeter at the tagged photon facility of the Mainz Microtron accelerator. Chiral perturbation theory (ChPT) predicts that close to threshold this channel is significantly enhanced compared to double pion final states with charged pions. In contrast to other reaction channels, the lower order tree terms are strongly suppressed in 2$\pi^0$ photoproduction. The consequence is the dominance of pion loops in the 2$\pi^0$ channel close to threshold - a result that opens new prospects for the test of ChPT and in particular its inherent loop terms. The present measurement is the first which is sensitive enough for a conclusive comparison with the ChPT calculation and is in agreement with its prediction. The data also show good agreement with a calculation in the unitary chiral approach.Comment: Submitted to PL

Perinatal asphyxia: CNS development and deficits with delayed onset

Perinatal asphyxia constitutes a prototype of obstetric complications occurring when pulmonary oxygenation is delayed or interrupted. The primary insult relates to the duration of the period lacking oxygenation, leading to death if not re-established. Re-oxygenation leads to a secondary insult, related to a cascade of biochemical events required for restoring proper function. Perinatal asphyxia interferes with neonatal development, resulting in long-term deficits associated to mental and neurological diseases with delayed clinical onset, by mechanisms not yet clarified. In the experimental scenario, the effects observed long after perinatal asphyxia have been explained by over expression of sentinel proteins, such as poly(ADP-ribose) polymerase-1 (PARP-1), competing for NAD+ during re-oxygenation, leading to the idea that sentinel protein inhibition constitutes a suitable therapeutic strategy. Asphyxia induces transcriptional activation of proinflammatory factors, in tandem with PARP-1 overactivation, and pharmacologically induced PARP-1 inhibition also down-regulates the expression of proinflammatory cytokines. Nicotinamide has been proposed as a suitable PARP-1 inhibitor. Its effect has been studied in an experimental model of global hypoxia in rats. In that model, the insult is induced by immersing rat foetuses into a water bath for various periods of time. Following asphyxia, the pups are delivered, treated, and nursed by surrogate dams, pending further experiments. Nicotinamide rapidly distributes into the brain following systemic administration, reaching steady state concentrations sufficient to inhibit PARP-1 activity for several hours, preventing several of the long-term consequences of perinatal asphyxia, supporting the idea that it constitutes a lead for exploring compounds with similar or better pharmacological profiles

How the other half lives: CRISPR-Cas's influence on bacteriophages

CRISPR-Cas is a genetic adaptive immune system unique to prokaryotic cells used to combat phage and plasmid threats. The host cell adapts by incorporating DNA sequences from invading phages or plasmids into its CRISPR locus as spacers. These spacers are expressed as mobile surveillance RNAs that direct CRISPR-associated (Cas) proteins to protect against subsequent attack by the same phages or plasmids. The threat from mobile genetic elements inevitably shapes the CRISPR loci of archaea and bacteria, and simultaneously the CRISPR-Cas immune system drives evolution of these invaders. Here we highlight our recent work, as well as that of others, that seeks to understand phage mechanisms of CRISPR-Cas evasion and conditions for population coexistence of phages with CRISPR-protected prokaryotes.Comment: 24 pages, 8 figure

Structural Invariants in Individuals Language Use: The "Ego Network" of Words

The cognitive constraints that humans exhibit in their social interactions have been extensively studied by anthropologists, who have highlighted their regularities across different types of social networks. We postulate that similar regularities can be found in other cognitive processes, such as those involving language production. In order to provide preliminary evidence for this claim, we analyse a dataset containing tweets of a heterogeneous group of Twitter users (regular users and professional writers). Leveraging a methodology similar to the one used to uncover the well-established social cognitive constraints, we find that a concentric layered structure (which we call ego network of words, in analogy to the ego network of social relationships) very well captures how individuals organise the words they use. The size of the layers in this structure regularly grows (approximately 2–3 times with respect to the previous one) when moving outwards, and the two penultimate external layers consistently account for approximately 60% and 30% of the used words (the outermost layer contains 100% of the words), irrespective of the number of the total number of layers of the user

News, intelligence and 'little lies' : rumours between the Cherokees and the British 1740-1785

Rumour and information are one of the most fundamental ways in which people engage with one another. Rumours can change the way that individuals and groups see each other and the actions that they take. Sociologists and anthropologists have long used rumour as a way to explore the experiences of their subjects. Historians of early America have, in recent years, begun to make use of rumour as a way of examining the, often hidden, world of interactions between American Indians and white Europeans. This thesis will expand upon this work by exploring the changing role of rumour within an intercultural relationship over several decades. This thesis will focus on rumour in the relationship between the Cherokee Nation and the colonists of the British Empire. It will explore the ways that rumour influenced these interactions and the impact of the rapidly changing backcountry environment of the latter eighteenth century, both on rumour and on the wider Cherokee- British relationship. This thesis will argue that rumour shifted in the course of the eighteenth century from being a diplomatic tool which could be used- either to create further panic and confusion or to calm and smooth over problems- to an uncontrollable force which would deepen and exacerbate the divisions between Cherokees and the British. Rumour played an important role in politics and society in the eighteenth century backcountry and its changing function offers a way to better understand the shifting currents of life in early America

Functional analysis of missense variants in the TRESK (KCNK18) K+ channel

A loss of function mutation in the TRESK K2P potassium channel (KCNK18), has recently been linked with typical familial migraine with aura. We now report the functional characterisation of additional TRESK channel missense variants identified in unrelated patients. Several variants either had no apparent functional effect, or they caused a reduction in channel activity. However, the C110R variant was found to cause a complete loss of TRESK function, yet is present in both sporadic migraine and control cohorts, and no variation in KCNK18 copy number was found. Thus despite the previously identified association between loss of TRESK channel activity and migraine in a large multigenerational pedigree, this finding indicates that a single non-functional TRESK variant is not alone sufficient to cause typical migraine and highlights the genetic complexity of this disorder