Quantitative analysis of pungent principles of pepper oleoresins: Comparative study of three analytical methods

For the determination of pungent compounds in pepper oleoresins, three analytical methods are described and compared to determine which of them is the most available for routine analysis and for informations about relative amount of all alkaloids. These methods are the UV spectrophotometry, the high-performance liquid-chromatography and the gas-chromatography. These analysis were carried out with 26 pepper oleoresins samples. The three methods were compared with two statistical tests, both defined in a French Norm from AFNOR. It seems that HPLC and GC methods, considering only the peak of piperine, are comparable. In the same way, HPLC and UV-spectrophotometry methods give similar results of total amount of alkaloids