7 research outputs found
Measuring long chain alkanes in diesel engine exhaust by thermal desorption PTR-MS
A method using thermal desorption sampling and analysis by proton transfer reaction mass spectrometry (PTR-MS) to measure
long chain alkanes (C12–C18) and other larger organics associated
with diesel engine exhaust emissions is described. Long chain alkanes undergo
dissociative proton transfer reactions forming a series of fragment ions with
formula CnH2n+1. The PTR-MS is insensitive to n-alkanes less than
C8 but displays an increasing sensitivity for larger alkanes. Fragment
ion distribution and sensitivity is a function of drift conditions. At 80 Td
the most abundant ion fragments from C10 to C16 n-alkanes were
m/z 57, 71 and 85. The mass spectrum of gasoline and diesel fuel at 80 Td
displayed ion group patterns that can be related to known fuel constituents,
such as alkanes, alkylbenzenes and cycloalkanes, and other compound groups
that are inferred from molecular weight distributions such as
dihydronapthalenes and naphthenic monoaromatics. It is shown that thermal
desorption sampling of gasoline and diesel engine exhausts at 80 Td allows
for discrimination against volatile organic compounds, allowing for
quantification of long chain alkanes from the abundance of CnH2n+1
fragment ions. The total abundance of long chain alkanes in diesel engine
exhaust was measured to be similar to the total abundance of C1–C4
alkylbenzene compounds. The abundance patterns of compounds determined by
thermal desorption sampling may allow for emission profiles to be developed
to better quantify the relative contributions of diesel and gasoline exhaust
emissions on organic compounds concentrations in urban air
Ralstonia solanacearum Needs Motility for Invasive Virulence on Tomato
Ralstonia solanacearum, a widely distributed and economically important plant pathogen, invades the roots of diverse plant hosts from the soil and aggressively colonizes the xylem vessels, causing a lethal wilting known as bacterial wilt disease. By examining bacteria from the xylem vessels of infected plants, we found that R. solanacearum is essentially nonmotile in planta, although it can be highly motile in culture. To determine the role of pathogen motility in this disease, we cloned, characterized, and mutated two genes in the R. solanacearum flagellar biosynthetic pathway. The genes for flagellin, the subunit of the flagellar filament (fliC), and for the flagellar motor switch protein (fliM) were isolated based on their resemblance to these proteins in other bacteria. As is typical for flagellins, the predicted FliC protein had well-conserved N- and C-terminal regions, separated by a divergent central domain. The predicted R. solanacearum FliM closely resembled motor switch proteins from other proteobacteria. Chromosomal mutants lacking fliC or fliM were created by replacing the genes with marked interrupted constructs. Since fliM is embedded in the fliLMNOPQR operon, the aphA cassette was used to make a nonpolar fliM mutation. Both mutants were completely nonmotile on soft agar plates, in minimal broth, and in tomato plants. The fliC mutant lacked flagella altogether; moreover, sheared-cell protein preparations from the fliC mutant lacked a 30-kDa band corresponding to flagellin. The fliM mutant was usually aflagellate, but about 10% of cells had abnormal truncated flagella. In a biologically representative soil-soak inoculation virulence assay, both nonmotile mutants were significantly reduced in the ability to cause disease on tomato plants. However, the fliC mutant had wild-type virulence when it was inoculated directly onto cut tomato petioles, an inoculation method that did not require bacteria to enter the intact host from the soil. These results suggest that swimming motility makes its most important contribution to bacterial wilt virulence in the early stages of host plant invasion and colonization