Fabrication of Poly (Evans Blue) Modified Graphite Paste Electrode as an Electrochemical sensor for Sensitive and Instant Riboflavin Detection

Electro-analysis of Riboflavin (RN) consumed as vitamin has been delved by constructing the poly (Evans Blue) modified graphite paste electrode (PEBMGPE) as an electrochemical detector. Voltammetric determination of RN in 0.2 M PBS (pH 7.0) at the electro-active layer of the PEBMGPE was conducted. The PEBMGPE plays a crucial role as sensing tool towards the detection of RN compared to the BGPE. The topographical features of the bare graphite paste electrode (BGPE) and PEBMGPE was acquired by the FESEM surface analysis. All experimental factors such as applied potential, the effect of scan rate, impact of the pH were optimized. Employing the Differential pulse voltammetry (DPV) approaches using the good experimental circumstances the analytical curve was linear in the range of 6×10-6-1.5×10-4 M,  the detection limit and limit of quantification gained as 2.1×10-7 M and 7.1×10-7 M, respectively. Subsequently, good repeatability, excellent reproducibility, and adequate stability, high sensitivity were achieved for the PEBMGPE sensor. In addition, the PEBMGPE sensor was applied for the interference study of RN with Dopamine (DA) and Paracetamol (PR). Besides the practicability of the projected sensor was successfully utilized for the detection of the RN in the pharmaceutical formulation

Enhanced voltammetric detection of paracetamol by using carbon nanotube modified electrode as an electrochemical sensor

New aspects associated with electro-catalytic activity of poly(methyl orange) modified carbon nanotube paste electrode (PMMCNTPE)towards the detection of paracetamol (PC) which is typically used worldwide as a pain reliever, were explored through implementation of cyclic voltammetry (CV) and differential pulse voltammetry (DPV) techniques. Bare carbon nanotube paste electrode (BCNTPE) was modified by methyl orange using the electropolymerizing method. The effect of pH and influence of potential scan rate were resolved by means of CV technique. It was found that under optimized experimental conditions, PMMCNTPE imparts the analytical curve for PC in the concentration range of 2.0×10-6 – 5.0×10-5 M with detection limit of 3.8×10-8 M and limit of quantification of 1.2×10-8 M. The proposed sensor exhibited acceptable reproducibility, admirable stability, and adequate repeatability. The interference study of PC with dopamine (DA) and folic acid (FA) showed good selectivity of the designed sensor. The feasibility of the constructed electrochemical sensor to detect PC was successfully tested in some pharmaceutical formulations

A study on patients treated with interlock nailing in the forearm fracture bones

Background: Fractures involving the bones of the forearm present unique problems not encountered with fractures of other long bones and may significantly affect the function of the upper limb. The purpose of the present study was to evaluate the functional outcome of patients treated with interlock nailing in the fracture forearm bones.Methods: Thirty two patients included after their consent. With the patient supine on a radiolucent table, and under general or regional anesthesia the extremity was prepared and the surgery was performed using a standard procedure. If secure rigid fixation is achieved forearm POP splint is applied and kept in place for 2 weeks, thereafter a removable sugar-tong orthosis is worn until bridging callus is present, and the orthosis is removed frequently for exercise.Results: The average age of the patients was 38.90 years. The major mode of injury was RTA (59.09%) followed by assault (36.36%). 41% of patients were operated within week of injury, only three patients were operated after a week and one patient after 3 weeks. More than half of patients had closed fractures and rest was open fractures, of which Gustilo Anderson type II were in majority. In 3/5 of patients locking at nondriving end was not done cause of stable fixation. There was statistically significant difference in the surgical time (P <0.05) and duration of postoperative immobilization differed statistically significantly (P <0.001) between the group of patients in whom locking was done and not done. Conclusions: Advantages of Interlocking nail are high rate of bony consolidation along with minimized surgical approaches, cosmetically better suited and little risk of refracture after removal of the implant

Evaluation of fungal pathogens, Fusarium semitectum Berk. and Ravenel and Hirsutella thompsonii Fisher against red spider mite, Tetranychus neocaledoni-cus (Andre) of okra under laboratory and greenhouse conditions

Efficacy of fungal pathogens, Fusarium semitectum Berk. and Ravenel and Hirsutella thompsonii Fisher was evaluated against red spider mite, Tetranychus neocaledonicus (Andre) of okra under laboratory and greenhouse conditions. Among the treatments evaluated under laboratory conditions, the highest mortality of 78.20 and 88.34 percent adults and immatures, respectively was recorded at 2.10 x 109 spores per ml of F. semitectum, whereas it was 82.05 and 92.31 per cent adults and immatures, respectively in case of H. thompsonii at 4.60 x 108 spores per ml. Among the twelve treatments evaluated under greenhouse conditions, H. thompsonii 4.60 x 108 spores per ml, F. semitectum 2.10 x 109 spores per ml + H. thompsonii 4.60 x 108 spores per ml, H. thompsonii 4.60 x 108 spores per ml + dicofol 0.025% and F. semitectum 2.10 x 109 spores per ml + H. thompsonii 4.60 x 108 spores per ml + dicofol 0.025% were found to be on par with each other with a cumulative percent mortality of 81.20, 81.48, 81.21 and 82.59 percent, respectively. By testing the field efficacy of F. semitectum and H. thompsonii against T. neocaledonicus, these fungal pathogens can be used as potential biocontrol agents for the sustainable management of mites on okra. Hence the present study evaluates the potentiality of F. semitectum for the management of T. neocaledonicus by comparing with the well-known acaricidal fungus, H. thompsonii

Determination of Phase Composition of Cobalt Nanoparticles Using 59Co Internal Field Nuclear Magnetic Resonance

It is well known that cobalt exhibits polymorphism, i.e., the co-existence of both the hcp and fcc phases. In particular, the method of synthesis and other thermodynamic conditions is known to play a crucial role in determining the particular phase of cobalt. In this work, we have compared the phase composition of the cobalt nanoparticles synthesized using two different solvents (water) and ethanol (Co@C). XRD measurements confirm the existence of fcc phase in commercial cobalt nanoparticles (Co@A), co-existence of fcc and hcp phases in Co@B, while the existence of the hcp phase in Co@C. We have studied these cobalt nanoparticles using 59Co internal field nuclear magnetic resonance (IFNMR) for verification of phase composition. Our studies reveal that the Co@A has fcc as a major phase with minor quantity hcp phase. Co@B exhibits approximately equal amount of fcc and hcp phase while Co@C exhibits hcp as a major phase with minor fcc phase. Our SEM micrograph studies confirm that the cobalt particles have spherical shape in the fcc phase. The cobalt particles exhibit both spherical and dendrite morphology confirming the co-existence of fcc and hcp phases, while the sample with pure hcp phase exhibits the dendrite morphology. Our studies also throw light on understanding the effect of solvent in the phase formation of the cobalt nanoparticles

Determination of Phase Composition of Cobalt Nanoparticles Using 59Co Internal Field Nuclear Magnetic Resonance

It is well known that cobalt exhibits polymorphism, i.e., the co-existence of both the hcp and fcc phases. In particular, the method of synthesis and other thermodynamic conditions is known to play a crucial role in determining the particular phase of cobalt. In this work, we have compared the phase composition of the cobalt nanoparticles synthesized using two different solvents (water) and ethanol (Co@C). XRD measurements confirm the existence of fcc phase in commercial cobalt nanoparticles (Co@A), co-existence of fcc and hcp phases in Co@B, while the existence of the hcp phase in Co@C. We have studied these cobalt nanoparticles using 59Co internal field nuclear magnetic resonance (IFNMR) for verification of phase composition. Our studies reveal that the Co@A has fcc as a major phase with minor quantity hcp phase. Co@B exhibits approximately equal amount of fcc and hcp phase while Co@C exhibits hcp as a major phase with minor fcc phase. Our SEM micrograph studies confirm that the cobalt particles have spherical shape in the fcc phase. The cobalt particles exhibit both spherical and dendrite morphology confirming the co-existence of fcc and hcp phases, while the sample with pure hcp phase exhibits the dendrite morphology. Our studies also throw light on understanding the effect of solvent in the phase formation of the cobalt nanoparticles

Post-thaw development of in vitro produced buffalo embryos cryopreserved by cytoskeletal stabilization and vitrification

The present study was conducted to examine post-thaw in vitro developmental competence of buffalo embryos cryopreserved by cytoskeletal stabilization and vitrification. In vitro produced embryos were incubated with a medium containing cytochalasin-b (cyto-b) in a CO2 incubator for 40 min for microfilament stabilization and were cryopreserved by a two-step vitrification method at 24℃ in the presence of cyto-b. Initially, the embryos were exposed to 10% ethylene glycol (EG) and 10% dimethylsulfoxide (DMSO) in a base medium for 4 min. After the initial exposure, the embryos were transferred to a 7 µl drop of 25% EG and 25% DMSO in base medium and 0.3 M sucrose for 45 sec. After warming, the embryos were cultured in vitro for 72 h. The post-thaw in vitro developmental competence of the cyto-b-treated embryos did not differ significantly from those vitrified without cyto-b treatment. The hatching rates of morulae vitrified without cyto-b treatment was significantly lower than the non-vitrified control. However, the hatching rate of cyto-b-treated vitrified morulae did not differ significantly from the non-vitrified control. This study demonstrates that freezing of buffalo embryos by cytoskeletal stabilization and vitrification is a reliable method for long-term preservation

Systematics of proton decay of actinides

255-262The phenomenon of proton emission from nuclear ground states limits the possibilities of the creation of more exotic proton rich nuclei that are usually produced by fusion-evaporation nuclear reactions. In the energy domain of radioactivity, proton can be considered as a point charge having highest probability of being present in the parent nucleus. Conclaves et al.1 studied the two-proton radioactivity of nuclei of mass number Aet al.2 reviewed the theories of proton emission to analyse the properties of nuclear matter. Maglione et al.3 analysed the proton emission from the some deformed nuclei. We have studied proton decay in almost all actinide nuclei. We have calculated the energy released during the proton decay (QP), penetration factor (P), and half-lives of proton decay. Proton decay half-lives are also longer than that of other decay modes such as alpha decay and spontaneous fission. To check the Geiger-Nuttal law for proton decay in actinide nuclei, we have plotted the logarithmic proton decay half-lives versus 1/sqrt(Q). The competition of proton decay with different decay modes such as alpha decay and spontaneous fission are also studied. We have also highlighted possible proton emitters with the corresponding energies and half-lives in the actinide region

Genetic Variability for Quantitative Traits in China Aster [Callistephus chinensis (L.) Nees]

A field study was conducted to estimate genetic variability, heritability and genetic advance in 20 genotypes of China aster for 15 traits during the year 2012-13 in Randomized Complete Block Design, with three replications. Results revealed that the magnitude of phenotypic co-efficient of variation (PCV) was higher than genotypic co-efficient of variation (GCV) for all the traits studied. Narrow differences between GCV and PCV were recorded in all the characters except flowering duration, vase-life and shelf-life, indicating little environmental influence on expression of these characters. High (>20%) GCV and PCV were recorded for plant height, number of branches and leaves per plant, flower diameter, number of ray and disc florets/flower head, stalk length, and, number and weight of flowers/plant. Heritability estimates ranged from 28.30% (flowering duration) to 99.54% (flower diameter). High heritability (<60%) was observed for all the traits except flowering duration. High heritability, coupled with high genetic advance as per cent mean, was recorded for flower diameter, stalk-length, number of branches/plant, weight of flowers/plant, days to first flower opening, days to 50 per cent flowering, plant height, number of leaves/plant, number of ray and disc florets/flower head, number of flowers/plant, indicating a possible role of additive gene action. Thus, these traits can be improved through selection and breeding

Determination of a complex crystal structure in the absence of single crystals : analysis of powder X-ray diffraction data, guided by solid-state NMR and periodic DFT calculations, reveals a new 2′-deoxyguanosine structural motif

Derivatives of guanine exhibit diverse supramolecular chemistry, with a variety of distinct hydrogen-bonding motifs reported in the solid state, including ribbons and quartets, which resemble the G-quadruplex found in nucleic acids with sequences rich in guanine. Reflecting this diversity, the solid-state structural properties of 3′,5′-bis-O-decanoyl-2′-deoxyguanosine, reported in this paper, reveal a hydrogen-bonded guanine ribbon motif that has not been observed previously for 2′-deoxyguanosine derivatives. In this case, structure determination was carried out directly from powder XRD data, representing one of the most challenging organic molecular structures (a 90-atom molecule) that has been solved to date by this technique. While specific challenges were encountered in the structure determination process, a successful outcome was achieved by augmenting the powder XRD analysis with information derived from solid-state NMR data and with dispersion-corrected periodic DFT calculations for structure optimization. The synergy of experimental and computational methodologies demonstrated in the present work is likely to be an essential feature of strategies to further expand the application of powder XRD as a technique for structure determination of organic molecular materials of even greater complexity in the future