Continuous Magnetophoretic Separation of Blood Cells from Plasma at the Microscale

We present a method for the direct and continuous separation of red and white blood cells from plasma at the microscale. The method is implemented in a microfluidic system with magnetic functionality. The fluidic structure within the microsystem consists of an inlet and a single microfluidic channel with multiple outlets. The magnetic functionality is provided by an array of integrated soft-magnetic elements that are embedded transverse and adjacent to the microchannel. The elements are magnetized using an external field, and once magnetized they produce a magnetic force on blood cells as they flow through the microchannel. In whole blood, white blood cells (WBCs) behave as diamagnetic microparticles, while red blood cells (RBCs) exhibit diamagnetic or paramagnetic behavior depending on the oxygenation of their hemoglobin. We study the motion of blood cells through the microchannel using a mathematical model that takes into account the magnetic, fluidic and gravitational forces on the cells. We use the model to study blood cell separation, and our analysis indicates that the microsystem is capable of separating WBC-rich plasma, deoxygenated RBC-rich plasma and cell-depleted plasma into respective outlets.Comment: Submitted to Journal of Applied Physic

Analysis of Particle Transport in a Magnetophoretic Microsystem

An analytical analysis is presented of the transport and capture of magnetic micro/nano-particles in a magnetophoretic microsystem that consists of an array of integrated soft-magnetic elements embedded beneath a microfluidic channel. The elements, which are polarized by a bias field, produce a nonuniform field distribution that gives rise to a force on magnetic particles within the microchannel. The equations governing particle motion are derived using analytical expressions for the dominant magnetic and fluidic forces. The magnetic force is obtained using an analytical expression for the field distribution in the microchannel combined with a linear magnetization model for the magnetic response of particles. The theory takes into account particle size and material properties, the bias field, the dimensions of the microchannel, the fluid properties, and the flow velocity. The equations of motion are solved to study particle transport and capture. The analysis indicates that the particles exhibit an oscillatory motion as they traverse the microsystem, and that a high capture efficiency can be obtained in practice

Integrated Geomechanical and Digital Photogrammetric Survey in the Study of Slope Instability Processes of a Flysch Sea Cliff (Debeli Rtič Promontory, Slovenia)

This work presents an integrated study approach that combines the results of a geomechanical survey with data obtained using digital photogrammetry (DP), to assess slope instability processes affecting a sea cliff at the Debeli Rtič promontory (Slovenia). The investigated cliff is 4–18 m-high and is made up of an alternation of sandstones and marlstones belonging to the Flysch Formation of Trieste, which is Eocene in age. The studied cliff was subjected to localized slope failures that occurred in the past and is currently subject to frequent rock collapses, thus resulting in its partial and episodic retreat. Field evidence acquired through a traditional survey was integrated with outputs of the DP technique based on 1399 images that were collected using both a commercial unmanned aerial vehicle (UAV) and a mobile phone (MP). UAV-derived images were useful for performing rock mass structure analysis in the upper part of the investigated cliff, where the traditional survey was not possible due to hazardous operating conditions. In addition, the use of a MP was observed to be a useful tool for the rapid collection of images at the toe of unsafe marine cliff environments. This study highlights that UAV-DP and MP-DP techniques can only be effective if the outcomes obtained from the 3D model reconstruction are validated by direct measurements acquired by means of the traditional field survey, thus avoiding improper or even erroneous results while enlarging the amount of data and the area of investigation. The study approach presented herein allowed for the assessment of slope instabilities affecting the Flysch Sea cliff, whose retreat is caused by the combined action of marine erosion and slope gravitational processes

Integrated Geomechanical and Digital Photogrammetric Survey in the Study of Slope Instability Processes of a Flysch Sea Cliff (Debeli Rtic Promontory, Slovenia)

This work presents an integrated study approach that combines the results of a geomechanical survey with data obtained using digital photogrammetry (DP), to assess slope instability processes affecting a sea cliff at the Debeli Rtic promontory (Slovenia). The investigated cliff is 4-18 m-high and is made up of an alternation of sandstones and marlstones belonging to the Flysch Formation of Trieste, which is Eocene in age. The studied cliff was subjected to localized slope failures that occurred in the past and is currently subject to frequent rock collapses, thus resulting in its partial and episodic retreat. Field evidence acquired through a traditional survey was integrated with outputs of the DP technique based on 1399 images that were collected using both a commercial unmanned aerial vehicle (UAV) and a mobile phone (MP). UAV-derived images were useful for performing rock mass structure analysis in the upper part of the investigated cliff, where the traditional survey was not possible due to hazardous operating conditions. In addition, the use of a MP was observed to be a useful tool for the rapid collection of images at the toe of unsafe marine cliff environments. This study highlights that UAV-DP and MP-DP techniques can only be effective if the outcomes obtained from the 3D model reconstruction are validated by direct measurements acquired by means of the traditional field survey, thus avoiding improper or even erroneous results while enlarging the amount of data and the area of investigation. The study approach presented herein allowed for the assessment of slope instabilities affecting the Flysch Sea cliff, whose retreat is caused by the combined action of marine erosion and slope gravitational processes

Automação de balanças comerciais como lisímetros em ambiente protegido.

bitstream/CNPDIA-2009-09/11843/1/CT80_2006.pd

N-isopropyl chitosan. A pH- and thermo-responsive polysaccharide for gel formation

The present contribution deals with the synthesis and characterization of N-isopropyl chitosan in which the introduction of hydrophobic groups leads to an increased flexibility of the polysaccharide backbone. The isopropyl groups extend the solubility of the modified-chitosan samples and render the modified chitosan a pH- and thermo-sensitive system for hydrogel formation. Indeed, upon varying the pH of the system and/or its temperature within a range compatible with biological applications, a non-reversible sol-gel transition occurs, as determined through extended rheological analyses. The modified chitosan samples show a very good biocompatibility as determined through preliminary viability and cell growth experiments

The Effect of the P/Si Ratio on the Preparation and Properties of Phosphoric Acid-Metakaolin Geopolymers

The present research deals with the production and characterization of geopolymers prepared by mixing metakaolin, different amounts of phosphoric acid solution and water. Hardening was performed by aging the geopolymeric pastes in a climatic chamber. The workability of the pastes has been improved while the H2O/total solid content has been kept constant and the P/Si ratio increased. However, such a benefit implies considerable heat output, which must be controlled in order to limit the formation of extended fractures. The compressive strength of the hardened materials increases with increasing P/Si ratio at a constant H2O/total solid content, whereas their strength decreases with increasing H2O/ total solid content at a constant P/Si ratio. The open macroporosity, which is directly dependent on the total amount of water added to the geopolymeric pastes, may explain the above results better than the nano/microporosity and/or chemical bonds that contribute to building up the materials\u2019 textural features