Nondestructive biomarkers in ecotoxicology.

The aim of this article is to attempt a concise review of the state of the art of the nondestructive biomarkers approach in vertebrates, establishing a consensus on the most useful and sensitive nondestructive biomarker techniques, and proposing research priorities for the development and validation of this promising methodology. The following topics are discussed: the advantages of the use of nondestructive strategies in biomonitoring programs and the research fields in which nondestructive biomarkers can be applied; the biological materials suitable for nondestructive biomarkers and residue analysis in vertebrates; which biomarkers lend themselves to noninvasive techniques; and the validation and implementation strategy of the nondestructive biomarker approach. Examples of applications of this methodology in the hazard assessment of endangered species are also presented

The short future of public broadcasting: Replacing digital terrestrial television with internet protocol?

According to recent European estimates, the life expectancy of broadcasting as a free-to-air television platform is not more than 15 years. BBC, Yle and the UK regulator Ofcom have reached this conclusion in their reports about the future of news, media distribution and digital terrestrial television (DTT). Although broadcasting is seen as necessary until 2030, all three assume that DTT can – under certain conditions – be replaced with delivery using internet protocol (IP). However, it seems that the idea of IPTV taking over DTT is just a sophisticated version of “black box fallacy”, driven by the expected growth of the new media ecosystem. The problems in replacing a socio-technological system have largely been neglected

Finland: Maintaining the fragile consensus

Peer reviewe

Risk factors of cervical intraepithelial lesion in Douala-Cameroon: Implications of Herpes Simplex Virus Type 2, Chlamydia Trachomatis and Treponema Pallidum

Infection with high risk oncogenic human papillomavirus (HPV) such as HPVs 16 and 18 is the main cause of cervical cancer. The objective of this study was to determine the impact of Chlamydia trachomatis, Herpes simplex virus 2 (HSV 2), Treponema pallidum and some sexual behaviour on malignant progression of cervical lesion in Douala, Cameroon. From July 2009 to January 2010, we performed routine cervical smears to 163 consenting women, who completed a questionnaire on risk factors of cervical cancer. Blood samples were obtained for each of these women and used for the detection of antibodies against Chlamydia trachomatis, HSV 2 and Treponema pallidum. Results obtained showed that 26/163 (17 LSIL and 9 HSIL) of women had abnormal cytology, 75.5% (123/163) had HSV 2 infection, 19% (31/163) infected by Chlamydia trachomatis and 4.3% (7/163) infected by Treponema pallidum. Among the LSIL-positive women 35.3% (6/17) and 94.1% (16/17) were infected with Chlamydia trachomatis and HSV 2 respectively. Among those with HSIL cytology, 22.2% (2/9), 66.7% (6/9) and 11.1% (1/9) respectively had Chlamydia trachomatis, HSV 2 and Treponema pallidum. High parity and pregnancy rate was observed among women with positive cytology. Our finding shown high rate of cervical abnormalities among women infected with HSV 2; and among those with a higher number of parities and pregnancies. These results suggest that further investigations should be made in Cameroon to access real burden of these risk factors in the progression and persistence of cervical lesion.Key words:risk factors, cervical cancer, HSV 2, Chlamydia trachomatis, sexually transmitted infections.doi: 10.4314/ajcem.v12i3.

Effects of copper on larvae of the marbled crab Pachygrapsus marmoratus (Decapoda, Grapsidae): toxicity test and biochemical marker responses

The importance of trace elements in ecotoxicological investigations is a well-known issue when monitoring polluted areas such as commercial harbors. Copper represents one of the most common metal contaminants, often detected in these areas as it is widely employed in various fields and has many sources of inflow in the marine environment. Pachygrapsus marmoratus is a widespread intertidal crab species that has been extensively studied in ecology, ethology and population genetics. Ecotoxicological studies have also been performed, exclusively on the adult stage. In the present study we investigated the mortality and biochemical (oxidative stress and neurotoxicity) responses of P. marmoratus larvae exposure to environmental relevant concentration of copper. Results showed dose-dependent responses in terms of larval mortality, with a calculated LC50 value of 0.5 mg/L of Cu2+. The LC50 concentration was used as the starting point for subsequent biochemical response evaluation. Results also demonstrated dose-dependent activation of antioxidant systems assuming a compensatory antioxidant activity to prevent higher cellular damage when larvae were exposed to the highest concentrations of copper. Moreover, a significant enhancement of neurotransmitter activities was observed, assuming a possible direct interaction of copper with the enzymes or an increase of free copper ion aliquot into the cells.publishe

Pirfenidone in idiopathic pulmonary fibrosis: real-life experience in the referral centre of Siena

Background: Idiopathic pulmonary fibrosis (IPF) is the most common idiopathic interstitial pneumonia and has a median survival after diagnosis of 2–5 years. Pirfenidone is the first approved antifibrotic drug for the treatment of IPF. Here we report the functional progress, side effects and survival data of a population of patients with IPF, diagnosed at our centre and treated with pirfenidone. Methods: We enrolled 91 patients with IPF (71 males) treated with pirfenidone. Clinical, survival and functional details were collected retrospectively at start of therapy and after 12, 24, 36 and 48 months of treatment. Lung function tests at least 12 months before starting therapy were available for 40 patients and were entered in the database, as well as side effects. Results: During the observation period (922 ± 529 days), 27 patients died, 5 patients underwent lung transplant and 10 patients interrupted therapy due to adverse events or IPF progression. The median survival was 1606 days. There was a significant reduction in disease progression rate, as measured by trend of forced vital capacity, after 1 year of therapy with respect to before treatment (p = 0.0085). Forced vital capacity reduction rate was progressively higher in the subsequent years of treatment. Treatment-related side effects were reported in 25 patients and were predominantly mild. Overall, four patients discontinued therapy due to severe photosensitivity. Conclusions: Our findings confirm the efficacy of pirfenidone in reducing functional progression of IPF and its excellent safety profile in a real-life setting. This study, designed on a long-term follow up, contributes to the growing evidence on safety, tolerability and efficacy of pirfenidone in IPF. The reviews of this paper are available via the supplemental material section

Collagen Type IV Alpha 5 Chain in Bronchiolitis Obliterans Syndrome After Lung Transplant: The First Evidence

Introduction: Bronchiolitis obliterans syndrome (BOS) is the most common form of CLAD and is characterized by airflow limitation and an obstructive spirometry pattern without parenchymal opacities. The protein signature of BOS lesions concerns extracellular matrix organization and aberrant basement membrane composition. In this pilot study, we investigated the presence of COL4A5 in the serum of patients with BOS. Methods: 41 patients who had undergone LTX were enrolled. Of these, 27 developed BOS and 14 (control group) were considered stable at the time of serum sampling. Of BOS patients, serum samples were analysed at the time of BOS diagnosis and before the clinical diagnosis (pre-BOS). COL4A5 levels were detected through the ELISA kit. Results: Serum concentrations of COL4A5 were higher in pre-BOS than in stable patients (40.5 ± 13.9 and 24.8 ± 11.4, respectively, p = 0.048). This protein is not influenced by comorbidities, such as acute rejection or infections, or by therapies. Survival analysis also reveals that a higher level of COL4A5 was also associated with less probability of survival. Our data showed a correlation between concentrations of COL4A5 and FEV1 at the time of diagnosis of BOS. Conclusion: Serum concentrations of COL4A5 can be considered a good prognostic marker due to their association with survival and correlation with functional parameters

The Structure and Properties of Solar Active Regions and Quiet-Sun Areas Observed in Soft X-Rays with Yohkoh/SXT and in the Extreme-Ultraviolet with SERTS

We observed two solar active regions (NOAA regions 7563 and 7565), quiet-Sun areas, and a coronal hole region simultaneously with Goddard Space Flight Center's Solar EUV Rocket Telescope and Spectrograph (SERTS) and with the Yohkoh Soft X-ray Telescope (SXT) on 1993 August 17. SERTS provided spatially resolved active region and quiet-Sun slit spectra in the 280 to 420 A wavelength range, and images in the lines of He II λ303.8, Mg IX λ368.1, Fe XV λ284.1, and Fe XVI λλ335.4 and 360.8 SXT provided images through multiple broadband filters in both the full-frame imaging mode and the partial-frame imaging mode. The SERTS images in Fe XV (log Tmax = 6.33, where Tmax is the temperature which maximizes the fractional ion abundance in the available ionization equilibrium calculations, i.e., the formation temperature) and Fe XVI (log Tmax = 6.43) exhibit remarkable morphological similarity to the SXT images. Whereas the Fe XV and XVI images outline the loop structures seen with SXT, the cooler He II (log Tmax = 4.67) and Mg IX (log Tmax = 5.98) images outline loop footpoints. In addition, the Mg IX emission outlines other structures not necessarily associated with the hot loops; these may be cool (T 1 × 106 K) loops. From the spatially resolved slit spectra, we obtained emission-line profiles for lines of He II λ303.8, Mg IX λ368.1, Fe XIII λ348.2, Si XI λ303.3, Fe XIV λ334.2, Fe XV λ284.1, and Fe XVI λ335.4 for each spatial position. Based upon the spatial variations of the line intensities, active region 7563 systematically narrows when viewed with successively hotter lines, and appears narrowest in the broadband soft X-ray emission. The active region width (full width at half-maximum intensity) diminishes linearly with log Tmax; the linear fit yields an extrapolated effective log Tmax of 6.51 ± 0.01 for the X-ray emission. The most intense, central core straddles the magnetic neutral line. Active region and quiet-Sun one-dimensional temperature scans were derived from intensity ratios of spatially resolved SERTS slit spectral lines, and from coregistered SXT filter ratios. The highest plasma temperatures were measured in the most intense, central core of region 7563. The temperatures derived from Fe XVI λ335.4/Fe XV λ284.1 and Fe XVI λ335.4/Fe XIV λ334.2 vary significantly (based upon the measurement uncertainties) but not greatly (factors of less than 1.5) across the slit. The average log T values derived from the above two ratios for region 7563 are 6.39 ± 0.04 and 6.32 ± 0.02, respectively. Somewhat larger systematic variations were obtained from all available SXT filter ratios. The average active region log T values derived from the SXT AlMgMn/thin Al, thick Al/thin Al, and thick Al/AlMgMn filter ratios are 6.33 ± 0.03, 6.45 ± 0.02, and 6.49 ± 0.03, respectively. Active region and quiet-Sun one-dimensional density scans were derived from intensity ratios of spatially resolved SERTS slit spectral lines of Fe XIII and Fe XIV. The derived densities show neither systematic nor significant variations along the slit in either the active region or the quiet-Sun, despite the fact that the intensities themselves vary substantially. This indicates that the product of the volume filling factor and the path length (fΔl) must be greater by factors of 3-5 in the active region core than in the outskirts. Furthermore, the derived active region densities are ~2 times the quiet-Sun densities. This density difference is adequate to explain the factor of ~4 intensity difference in Fe XII and Fe XIII between the active and quiet areas, but it is not adequate to explain the factor of ~8 intensity difference in Fe XIV between the active and quiet areas. We attribute the latter to a greater fΔl in the active regions. Statistically significant Doppler shifts are not detected in region 7563 or in the quiet-Sun with any of the EUV lines