14 research outputs found
Manipulation of Phytic acid and Raffinosaccharides content in bean seed: isolation of mutants and molecular analysis of myo-inositol-3-phosphate synthase.
Isolation and Characterisation of an lpa (low phytic 1 acid) Mutant in Common Bean (Phaseolus vulgaris L.)
Phytic acid is considered as one of the major antinutritional compounds in cereal and legume seeds. The development of lpa (low phytic acid) grains, resulting in increased
mineral cation availability, is considered a major goal in the improvement of the nutritional quality of seed crops, especially those largely consumed in developing countries. From a mutagenised population of common bean we isolated a homozygous lpa mutant line (lpa-280-10) showing, compared to wild type, a 90% reduction of phytic acid, a 25% reduction of raffinosaccharides and a much higher amount of free or weakly
bound iron cations in the seed. Genetic analysis showed that the lpa character is due to a recessive mutation that segregates in a monogenic, Mendelian fashion. Germination tests, performed using varying ageing or stress conditions, clearly showed that the bean line lpa-280-10 has a better germination response than the wild type. These data, together with those obtained from two years of agronomic trials showing that the mutant seed yield is close to that of its parents, and other evidence, indicate that the new lpa-280-10 mutation might be the first devoid of visible macroscopic negative effects in plants, pods and seeds