Iron(III)-Salophene: An Organometallic Compound with Selective Cytotoxic and Anti-Proliferative Properties in Platinum-Resistant Ovarian Cancer Cells

Background: In this pioneer study to the biological activity of organometallic compound Iron(III)-salophene (Fe-SP) the specific effects of Fe-SP on viability, morphology, proliferation, and cell-cycle progression on platinum-resistant ovariancancer cell lines were investigated. Methodology/Principal Findings: Fe-SP displayed selective cytotoxicity against SKOV-3 and OVCAR-3 (ovarian epithelial adenocarcinoma) cell lines at concentrations between 100 nM and 1 μM, while the viability of HeLa cells (epithelial cervix adenocarcinoma) or primary lung or skin fibroblasts was not affected. SKOV-3 cells in contrast to fibroblasts after treatment with Fe-SP revealed apparent hallmarks of apoptosis including densely stained nuclear granular bodies within fragmented nuclei, highly condensed chromatin and chromatin fragmentation. Fe-SP treatment led to the activation of markers of the extrinsic (Caspase-8) and intrinsic (Caspase-9) pathway of apoptosis as well as of executioner Caspase-3 while PARP-1 was deactivated. Fe-SP exerted effects as an anti-proliferative agent with an IC50 value of 300 nM and caused delayed progression of cells through S-phase phase of the cell cycle resulting in a complete S-phase arrest. When intra-peritoneally applied to rats Fe-SP did not show any systemic toxicity at concentrations that in preliminary trials were determined to be chemotherapeutic relevant doses in a rat ovarian cancer cell model. Conclusion/Significance: The present report suggests that Fe-SP is a potent growth-suppressing agent in vitro for cell lines derived from ovarian cancer and a potential therapeutic drug to treat such tumors in viv

Happiness, Excellence, and Optimal Human Functioning Revisited: Examining the Peer-Reviewed Literature Linked to Positive Psychology

Since the original call by Seligman and Csikszentmihalyi (2000) for a new science of happiness, excellence, and optimal human functioning, there has been an explosion of activity in, acclaim for, and criticism of positive psychology. The purpose of this study was to identify and examine the peer-reviewed literature linked to the positive psychology movement. An extensive systematic review identified 1336 articles published between 1999 and 2013. More than 750 of these articles included empirical tests of positive psychology theories, principles, and interventions. The results show a fairly consistent increase in the rate of publication, and that the number of empirical studies has grown steadily over the time period. The findings demonstrate that positive psychology is a growing and vibrant sub-area within the broader discipline of psychology, committed to using the same rigorous scientific methods as other sub-areas, in pursuit of understanding well-being, excellence, and optimal human functioning