USE OF FERMENTED CASSAVA, PALM KERNEL CAKE AND DRIED BREWERS«¤?? GRAINS TO PRODUCE MAIZE- FREE LOW-COST DIETS FOR LAYING HENS

An experiment was carried out to determine the performance of laying hens fed maize-free diets based on combinations of fermented cassava tuber meal (FCMT), palm kernel cake (PKC) and dried brewers«¤?? grains (DBG) as source of energy. Diet I (control) contained maize as the main source of energy while diets 2, 3, 4, 5, and 6 contained FCTM, PKC and DBG at percentages of 30, 20, 20; 35, 15, 20; 35, 20, 15; 25, 20, 25 and 25, 25 and 20, respectively, completely eliminating maize. Each diet was fed to a group of 24 laying hens for 12 weeks, using completely randomized design (CRD). The layers on the maize-free diets tended to consume more feed than those on the control diet. The group on diet 3 (35%: 15%: 20%) recorded significantly (P<0.05) higher hen-day egg production (69.50%) than those on the other diets. There were no significant differences (P>0.05) in egg weights. The group on the control diet and that on diet 3 recorded significantly (P<0.05) superior feed conversion ratios. Haugh unit of the eggs from diet 4 was significantly (P<0.05) higher than the others. Diets 3 recorded the lowest feed cost of egg production with the value of N178.67 per kg eggs as against N210.25 per kg eggs from the control. The results of the trial have shown that fermented cassava tuber meal, palm kernel cake and dried brewers«¤?? grains can be used to produce maize-fee low-cost diets for laying hens if properly combined

USE OF FERMENTED CASSAVA, PALM KERNEL CAKE AND DRIED BREWERS’ GRAINS TO PRODUCE MAIZEFREE LOW-COST DIETS FOR LAYING HENS

An experiment was carried out to determine the performance of laying hens fed maize-free diets based on combinations of fermented cassava tuber meal (FCMT), palm kernel cake (PKC) and dried brewers’ grains (DBG) as source of energy. Diet I (control) contained maize as the main source ofenergy while diets 2, 3, 4, 5, and 6 contained FCTM, PKC and DBG at percentages of 30, 20, 20; 35, 15, 20; 35, 20, 15; 25, 20, 25 and 25, 25 and 20, respectively, completely eliminating maize. Each diet was fed to a group of 24 laying hens for 12 weeks, using completely randomized design (CRD). Thelayers on the maize-free diets tended to consume more feed than those on the control diet. The group on diet 3 (35%: 15%: 20%) recorded significantly (P<0.05) higher hen-day egg production (69.50%) than those on the other diets. There were no significant differences (P>0.05) in egg weights. Thegroup on the control diet and that on diet 3 recorded significantly (P<0.05) superior feed conversion ratios. Haugh unit of the eggs from diet 4 was significantly (P<0.05) higher than the others. Diets 3 recorded the lowest feed cost of egg production with the value of N178.67 per kg eggs as against N210.25 per kg eggs from the control. The results of the trial have shown that fermented cassava tuber meal, palm kernel cake and dried brewers’ grains can be used to produce maize-fee low-costdiets for laying hens if properly combined

Effect of Dietary Pumpkin Stem Waste on Haematological Indices, Meat and Serum Lipid Profiles of Rabbits

This study evaluated the effect of different supplementary dietary levels of pumpkin stem waste (PSW) on the haematological indices, meat and serum lipid profile of rabbits. Three experimental feed regime:D1 (control), basal feed+ 0% PSW; D2,basal feed + 5% PSW and D3, basal feed + 10% PSW were randomly assigned to the three treatment groups in a 3x2 factorial arrangement in a completely randomized design experiment. The PSW percentage were offered on rabbit weight basis. Thirty six (18 males and 18 females) crossbred (California x New Zealand white) weaned rabbits aged 6 and 7 weeks were divided into three treatment groups of 12 rabbits each with 4 rabbits per replicate (2 females and 2 males) and randomly allotted to the three dietary treatments. At the end of 12 weeks feeding trial( prior to slaughtering), blood samples were collected from 6 rabbits (3 males and 3 females) per treatment totalling 18 rabbits and used to determine the serum lipid profile and haematological blood indices. Similarly, breast meat sample was used to determined lipid profile. Results obtained showed that while red blood cell (RBC),haemoglobin (Hb), packed cell volume (PCV),Mean cell haemoglobin (MCH) and mean cell haemoglobin concentration MCHC increased (P<0.05) with increasing dietary PSW. White blood cell (WBC) and MCHC however showed no significant difference (P>0.05) among the treatments groups. Lipid profile of both serum and meat indicated that HDL increased significantly (P<0.05) with increase in PSW while total cholesterol (TC), low density lipoprotein (LDL), triglyceride (TG) and very low density lipoprotein (VLDL) decreased significantly (P<0.05). Diet by sex interaction showed that male rabbits recorded significantly different (P<0.05) values from their female counterparts in all the blood and meat lipid parameters assessed. These results suggest that pumpkin stem waste is hypolipidaemic therefore could lower rabbits’ meat cholesterol and improve blood constituents

A nano-enabled cancer-specific ITCH RNAi chemotherapy booster for pancreatic cancer

UNLABELLED: Gemcitabine is currently the standard therapy for pancreatic cancer. However, growing concerns over gemcitabine resistance mean that new combinatory therapies are required to prevent loss of efficacy with prolonged treatment. Here, we suggest that this could be achieved through co-administration of RNA interference agents targeting the ubiquitin ligase ITCH. Stable anti-ITCH siRNA and shRNA dendriplexes with a desirable safety profile were prepared using generation 3 poly(propylenimine) dendrimers (DAB-Am16). The complexes were efficiently taken up by human pancreatic cancer cells and produced a 40-60% decrease in ITCH RNA and protein expression in vitro (si/shRNA) and in a xenograft model of pancreatic cancer (shRNA). When co-administered with gemcitabine (100 mg/kg/week) at a subtherapeutic dose, treatment with ITCH-shRNA (3x 50 mg/week) was able to fully suppress tumour growth for 17 days, suggesting that downregulation of ITCH mediated by DAB-Am16/shRNA sensitizes pancreatic cancer to gemcitabine in an efficient and specific manner. FROM THE CLINICAL EDITOR: Gemcitabine delivery to pancreatic cancer often results in the common problem of drug resistance. This team overcame the problem through co-administration of siRNA and shRNA dendriplexes targeting the ubiquitin ligase ITCH

Clustering superparamagnetic iron oxide nanoparticles produces organ-targeted high-contrast magnetic resonance images

AIM: Superparamagnetic iron oxide nanoparticles (SPIONs) have been used as magnetic resonance imaging (MRI) contrast agents; however, a number of T2-weighted imaging SPIONs have been withdrawn due to their poor clinical contrast performance. Our aim was to significantly improve SPION T2-weighted MRI contrast by clustering SPIONs within novel chitosan amphiphiles. METHODS: Clustering SPIONs was achieved by encapsulation of hydrophobic-coated SPIONs with an amphiphilic chitosan polymer (GCPQ). RESULTS: Clustering increases the spin-spin (r2) to spin-lattice (r1) relaxation ratio (r2/r1) from 3.0 to 79.1, resulting in superior contrast. Intravenously administered clustered SPIONs accumulated only in the liver and spleen; with the reduction in T2 relaxation confined, in the liver, to the extravascular space, giving clear MRI images of the liver vasculature

Oral particle uptake and organ targeting drives the activity of amphotericin B nanoparticles

There are very few drug delivery systems that target key organs via the oral route, as oral delivery advances normally address gastrointestinal drug dissolution, permeation, and stability. Here we introduce a nanomedicine in which nanoparticles, while also protecting the drug from gastric degradation, are taken up by the gastrointestinal epithelia and transported to the lung, liver, and spleen, thus selectively enhancing drug bioavailability in these target organs and diminishing kidney exposure (relevant to nephrotoxic drugs). Our work demonstrates, for the first time, that oral particle uptake and translocation to specific organs may be used to achieve a beneficial therapeutic response. We have illustrated this using amphotericin B, a nephrotoxic drug encapsulated within <i>N</i>-palmitoyl-<i>N</i>-methyl-<i>N</i>,<i>N</i>-dimethyl-<i>N</i>,<i>N</i>,<i>N</i>-trimethyl-6-<i>O</i>-glycol chitosan (GCPQ) nanoparticles, and have evidenced our approach in three separate disease states (visceral leishmaniasis, candidiasis, and aspergillosis) using industry standard models of the disease in small animals. The oral bioavailability of AmB-GCPQ nanoparticles is 24%. In all disease models, AmB-GCPQ nanoparticles show comparable efficacy to parenteral liposomal AmB (AmBisome). Our work thus paves the way for others to use nanoparticles to achieve a specific targeted delivery of drug to key organs via the oral route. This is especially important for drugs with a narrow therapeutic index

A cost-effectiveness analysis of provider and community interventions to improve the treatment of uncomplicated malaria in Nigeria: study protocol for a randomized controlled trial.

BACKGROUND: There is mounting evidence of poor adherence by health service personnel to clinical guidelines for malaria following a symptomatic diagnosis. In response to this, the World Health Organization (WHO) recommends that in all settings clinical suspicion of malaria should be confirmed by parasitological diagnosis using microscopy or Rapid Diagnostic Test (RDT). The Government of Nigeria plans to introduce RDTs in public health facilities over the coming year. In this context, we will evaluate the effectiveness and cost-effectiveness of two interventions designed to support the roll-out of RDTs and improve the rational use of ACTs. It is feared that without supporting interventions, non-adherence will remain a serious impediment to implementing malaria treatment guidelines. METHODS/DESIGN: A three-arm stratified cluster randomized trial is used to compare the effectiveness and cost-effectiveness of: (1) provider malaria training intervention versus expected standard practice in malaria diagnosis and treatment; (2) provider malaria training intervention plus school-based intervention versus expected standard practice; and (3) the combined provider plus school-based intervention versus provider intervention alone. RDTs will be introduced in all arms of the trial. The primary outcome is the proportion of patients attending facilities that report a fever or suspected malaria and receive treatment according to malaria guidelines. This will be measured by surveying patients (or caregivers) as they exit primary health centers, pharmacies, and patent medicine dealers. Cost-effectiveness will be presented in terms of the primary outcome and a range of secondary outcomes, including changes in provider and community knowledge. Costs will be estimated from both a societal and provider perspective using standard economic evaluation methodologies. TRIAL REGISTRATION: Clinicaltrials.gov NCT01350752

Examining equity in access to long-lasting insecticide nets and artemisinin-based combination therapy in Anambra state, Nigeria

<p>Abstract</p> <p>Background</p> <p>In order to achieve universal health coverage, the government of Anambra State, southeast Nigeria has distributed free Long-lasting Insecticide treated Nets (LLINs) to the general population and delivered free Artemisinin-based Combination Therapy (ACT) to pregnant women and children less than 5 years. However, the levels of coverage with LLINS and ACTs is not clear, especially coverage of different socio-economic status (SES) population groups. This study was carried out to determine the level of coverage and access to LLINs and ACTs amongst different SES groups.</p> <p>Methods</p> <p>A questionnaire was used to collect data from randomly selected households in 19 local government areas of the State. Selected households had a pregnant woman and/or a child less than 5 years. The lot quality assurance sampling (LQAS) methodology was used in sampling. The questionnaire explored the availability and utilization of LLINs and ACTs from 2394 households. An asset-based SES index was used to examine the level of access of LLINS and ACTs to different SES quintiles.</p> <p>Results</p> <p>It was found that 80.5 % of the households had an LLIN and 64.4 % of the households stated that they actually used the nets the previous night. The findings showed that 42.3 % of pregnant women who had fever within the past month received ACTs, while 37.5 % of children ≪5 years old who had malaria in the past month had received ACTs. There was equity in ownership of nets for the range 1–5 nets per household. No significant SES difference was found in use of ACTs for treatment of malaria in children under five years old and in pregnant women.</p> <p>Conclusions</p> <p>The free distribution of LLINs and ACTs increased household coverage of both malaria control interventions and bridged the equity gap in access to them among the most vulnerable groups.</p

Influence of FTO rs9939609 and Mediterranean diet on body composition and weight loss: a randomized clinical trial

Background The Mediterranean diet (MeD) plays a key role in the prevention of obesity. Among the genes involved in obesity, the Fat mass and obesity-associated gene (FTO) is one of the most known, but its interaction with MeD remained uncertain so far. Methods We carried out a study on a sample of 188 Italian subjects, analyzing their FTO rs9939609 alleles, and the difference in body composition between the baseline and a 4-weeks nutritional intervention. The sample was divided into two groups: the control group of 49 subjects, and the MeD group of 139 subjects. Results We found significant relations between MeD and both variation of total body fat (ΔTBFat) (p = 0.00) and gynoid body fat (p = 0.04). ∆TBFat (kg) demonstrated to have a significant relation with the interaction diet-gene (p = 0.04), whereas FTO was associated with the variation of total body water (p = 0.02). Conclusions MeD demonstrated to be a good nutritional treatment to reduce the body fat mass, whereas data about FTO remain uncertain. Confirming or rejecting the hypothesis of FTO and its influence on body tissues during nutritional treatments is fundamental to decide whether its effect has to be taken into consideration during both development of dietetic plans and patients monitoring. Trial Registration ClinicalTrials.gov Id: NCT01890070. Registered 01 July 2013, https://clinicaltrials.gov/ct2/show/NCT0189007