ASSESSMENT OF IMPORTANT TECHNOLOGICAL PARAMETERS OF NEW MOROCCAN DOMESTICATED TETRAPLOID OAT LINES OF AVENA MAGNA

Results of studying the oat collection of the N. I. Vavilov All-Russian Institute of Plant Genetic Resources are presented. Field and Nine tetraploid oat lines of Avena magna Murph. et Terr. were assessed for their technological performance. Physicochemical analyses were performed, including moisture, ash, proteins, fibre fractions, lipids, carbohydrates, and minerals. Statistical analysis revealed noteworthy differences in the chemical composition between the cultivars

Variation of some physical and chemical quality traits of Moroccan domesticated tetraploid oat lines of <i>Avena murphyi</i> Ladiz.

A potential in improving nutrition and health is the consumption of high balanced whole grains. A breeding program was launched by the National Institute for Agricultural Research (INRA-Morocco), aiming to develop new domesticated tetraploid oat lines of Avena murphyi Ladiz., with high nutritional benefits. A sequence-based diversity study was conducted on ten tetraploid oat lines of A. murphyi to shed light not only on the importance of domesticating wild oat species for crop improvement but also to highlight the nutritional traits of those oat lines. In this study, we assessed the lines for some grain nutritional traits, such as groat contents of proteins and lipids as well as ash, fiber fractions, carbohydrates, and minerals.The obtained results showed a wide range of chemical contents among lines. The results revealed a high significant difference (P &lt; 0.001) in the groat contents of proteins (11.46–15.12%), fat (4.14–10.14%), carbohydrates (48.68–57.38%), and ash (2.71–5.18%). Analysis of total fiber fractions (NDF, ADF, ADL and CF), showed the presence of significant differences between the assessed lines. The lines A. murphyi 8 and 9, recorded the highest groat protein contents of 15.12% and 13.66%, with an interesting macroelement profile, mainly magnesium and phosphorus, and iron and manganese as minor mineral profile.Due to their biochemical composition, Moroccan domesticated tetraploid oat lines of A. murphyi offer many opportunities to improve oat cultivation in Morocco and serve as an excellent raw material for foodstuffs formulation

A rapid and accurate 3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide colorimetric assay for quantification of bacteriocins with nisin as an example*

The objective of this study is to propose a more accurate and faster MTT [3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide] colorimetric assay (MCA) for quantitative measurement of polypeptide bacteriocins in solutions with nisin as an example. After an initial incubation of nisin and indicator bacterium Micrococcus luteus NCIB 8166 in tubes, MTT was added for another incubation period. After that, nisin was quantified by estimating the number of viable bacteria based on measuring the amount of purple formazan produced by cleavage of yellow tetrazolium salt MTT. Then MCA was compared to a standard agar diffusion assay (ADA). The results suggested a high correlation coefficient (r 2=0.975±0.004) between optical density (OD) and the inhibitory effect of nisin on a bacterial strain Micrococcus luteus NCIB 8166 at a range of 0.125~32 IU/ml. The MCA described in this study was very quick. Quantification of nisin took only 7~8 h and the detection limit was at the level of 0.125 IU/ml when compared to 12 IU/ml and 24~28 h for ADA. The MCA provides an accurate and rapid method for quantification of nisin in solutions and is expected to be used for quantification of other antimicrobial substances