Educational Activities to Help Transferring Knowledge in Nuclear: The Seminars of Spanish Young Generation in Nuclear (Jóvenes Nucleares)

From its creation, Spanish Young Generation in Nuclear (Jóvenes Nucleares, JJNN), a non-profit organization that depends on the Spanish Nuclear Society (SNE), has as an important scope to help transferring the knowledge between those generations in the way that it can be possible

From Secondary School To University: Attracting Young Students Towards A Career In Nuclear

From its creation, Spanish Young Generation in Nuclear (Jóvenes Nucleares, JJNN), a non-profit organization that depends on the Spanish Nuclear Society (SNE), has as an important scope to help spread knowledge about nuclear energy, not only pointing out its advantages and its role in our society, but also trying to correct some of the ideas that are due to the biased information and to the lack of knowledge. To try to have success in that goal, some high school lectures were taught and it has been organized regularly a Basic Course on Nuclear Science and Technolog

The enzymes LSD1 and Set1A cooperate with the viral protein HBx to establish an active hepatitis B viral chromatin state

Indexación: Web of ScienceWith about 350 million people chronically infected around the world hepatitis B is a major health problem. Template for progeny HBV synthesis is the viral genome, organized as a minichromosome (cccDNA) inside the hepatocyte nucleus. How viral cccDNA gene expression is regulated by its chromatin structure; more importantly, how the modulation of this structure impacts on viral gene expression remains elusive. Here, we found that the enzyme SetDB1 contributes to setting up a repressed cccDNA chromatin state. This repressive state is activated by the histone lysine demethylase-1 (LSD1). Consistently, inhibiting or reducing LSD1 levels led to repression of viral gene expression. This correlates with the transcriptionally repressive mark H3K9 methylation and reduction on the activating marks H3 acetylation and H3K4 methylation on viral promoters. Investigating the importance of viral proteins we found that LSD1 recruitment to viral promoters was dependent on the viral transactivator protein HBx. Moreover, the histone methyltransferase Set1A and HBx are simultaneously bound to the core promoter, and Set1A expression correlates with cccDNA H3K4 methylation. Our results shed light on the mechanisms of HBV regulation mediated by the cccDNA chromatin structure, offering new therapeutic targets to develop drugs for the treatment of chronically infected HBV patients.http://www.nature.com/articles/srep2590

Estimation of non-linear site response in a deep Alpine valley

We simulate non-linear behaviour of soils during strong ground motion in the Rhône valley in southern Switzerland. Previous studies of the site response using weak ground motion, ambient noise and linear 3-D FD simulations suggest that the 2-D structure of the basin will lead to amplification factors of up to 12 in the frequency band between 0.5 and 10 Hz. To estimate the importance of non-linear soil behaviour during strong ground motion in the Rhône valley we simulate the response of a superficial soft layer with a fully non-linear 1-D finite difference code. The non-linear wave propagator is based on an effective stress constitutive soil model capable of predicting pore pressure evolution due to shear. We determine the required dilatancy parameters from laboratory analysis of soil samples using cyclic triaxial tests. In order to include the effect of the strong 2-D structure in our non-linear analysis synthetic seismograms are convolved with the transfer function of the basin and then propagated through a 1-D non-linear layer. We find that reduced amplification due to soil non-linearity can be expected at rock accelerations above 0.5 ms−2, and that de-amplification occurs at ground motion levels of approximately 2 ms−2. Nevertheless, the spectral accelerations simulated for the valley centre are still exceeding the design spectra at about 0.5 Hz for magnitudes above 6.0, which reflects the strong amplification of ground motion by the deep 2-D resonance of the basin. For frequencies above 1 Hz the design spectra are generally in agreement with the strongest simulated accelerations. We evaluate the occurrence of soil failure using the 5 per cent strain criterion as a function of hypocentral distance and magnitude. Results confirm observations of liquefaction reported after the 1855 Mw 6.4 earthquake of Visp, and they suggest that soil liquefaction may occur at distances beyond those predicted by empirical relations in the valley. Near the basin edge, however, the simulated liquefaction occurrence agrees with the empirical relations. These results suggest that the response of the whole structure needs to be simulated in order to estimate the non-linear seismic response of complex basins like the Rhône valle

Stress compensation by gap monolayers for stacked InAs/GaAs quantum dots solar cells

In this work we report the stacking of 10 and 50 InAs quantum dots layers using 2 monolayers of GaP for stress compensation and a stack period of 18 nm on GaAs (001) substrates. Very good structural and optical quality is found in both samples. Vertical alignment of the dots is observed by transmission electron microscopy suggesting the existence of residual stress around them. Photocurrent measurements show light absorption up to 1.2 μm in the nanostructures together with a reduction in the blue response of the device. As a result of the phosphorus incorporation in the barriers, a very high thermal activation energy (431 meV) has also been obtained for the quantum dot emission

Phenotyping biotic-abiotic interactions affecting rice grain yield to discover tolerant genotypes

High temperatures are known to exacerbate rice panicle blight caused by Burkholderia glumae. Our long term goal is to develop rice with increased tolerance to combined heat and disease stresses. Our first steps have been to develop a robust experimental system to test how heat stress tolerance interacts with B. glumae infection. A set of rice genotypes with contrasting tolerance to heat was inoculated with B. glumae at anthesis stage. Genotype response to bacterial infection was measured by quantifying the proportion of empty spikelets as well as the total number of grains obtained from inoculated panicles. Preliminary results identified promising rice genotypes that can tolerate heat stress and the infection by B. glumae separately. These rice genotypes will be valuable for studying the molecular mechanisms responsible for tolerance and enable development of new rice varieties that withstand combined stresses under field conditions

Gene expression analyses determine two different subpopulations in KIT-negative GIST-like (KNGL) patients

Introduction: There are limited findings available on KIT-negative GIST-like (KNGL) population. Also, KIT expression may be post-transcriptionally regulated by miRNA221 and miRNA222. Hence, the aim of this study is to characterize KNGL population, by differential gene expression, and to analyze miRNA221/222 expression and their prognostic value in KNGL patients. Methods: KIT, PDGFRA, DOG1, IGF1R, MIR221 and MIR222 expression levels were determined by qRT-PCR. We also analyzed KIT and PDGFRA mutations, DOG1 expression, by immunohistochemistry, along with clinical and pathological data. Disease-free survival (DFS) and overall survival (OS) differences were calculated using Log-rank test. Results: Hierarchical cluster analyses from gene expression data identified two groups: group I had KIT, DOG1 and PDGFRA overexpression and IGF1R underexpression and group II had overexpression of IGF1R and low expression of KIT, DOG1 and PDGFRA. Group II had a significant worse OS (p = 0.013) in all the series, and showed a tendency for worse OS (p = 0.11), when analyzed only the localized cases. MiRNA222 expression was significantly lower in a control subset of KIT-positive GIST (p < 0.001). OS was significantly worse in KNGL cases with higher expression of MIR221 (p = 0.028) or MIR222 (p = 0.014). Conclusions: We identified two distinct KNGL subsets, with a different prognostic value. Increased levels of miRNA221/222, which are associated with worse OS, could explain the absence of KIT protein expression of most KNGL tumors

Evaluación y rehabilitación neuropsicológica en oncología pediátrica.

Objetivo. El presente trabajo tiene como objetivo el estudio de los efectos neuropsicológicos a largo plazo del cáncer infantil y sus tratamientos así como plantear propuestas para la rehabilitación cognitiva. Método. El método ha consistido en una revisión sistemática de los resultados en la literatura científica acerca de los efectos de la quimioterapia y la radioterapia sobre el SNC y en las funciones cognitivas y conductuales detectados a través de estudios de neuroimagen como calcificaciones y cambios en la sustancia blanca. Presentamos, además, el protocolo de evaluación neuropsicológica ejemplificado con un diagnóstico frecuente que presenta deterioro generalizado. Proponemos un programa de rehabilitación neuropsicológica basado en el Attention Process Training (APT) de Mateer y Sohlberg. Resultado. La radioterapia craneal y el metotrexato producen alteraciones generalizadas que se reflejan en déficit neuropsicológicos: CI, atención, memoria, habilidades académicas y motoras. Conclusiones. El avance en los tratamientos ha supuesto la disminución del número e intensidad de las secuelas. No obstante, sigue siendo frecuente que en los tumores del SNC y en las leucemias aparezcan alteraciones cognitivas y conductuales. La evaluación neuropsicológica continua y los modelos de sustancia blanca nos ayudan a entender estos efectos a largo plazo. Asimismo, esta evaluación es fundamental para diagnosticar los déficit evitando falsas atribuciones y expectativas inadecuadas. Destacamos la necesidad de una unidad de seguimiento y del trabajo interdisciplinario para promover la calidad de vida de los niños con cáncer