PHÂN TÍCH TRÌNH TỰ GIEN PHES CHO VIỆC XÁC ĐỊNH LOÀI VI KHUẨN LACTIC SINH BACTERIOCIN

PHES GENE SEQUENCE ANALYSIS FOR THE IDENTIFICATION OF A LACTIC ACID BACTERIUM PRODUCING  BACTERIOCIN FROM NEM CHUA The aim of this study was to identify species level of producing bacteriocin strain isolated from Ha Noi nem chua (DV4.10) by pheS gene sequencing analysis. Genomic DNA DV4.10 was extracted. Then pheS gene was run the first PCR with PheS-21-F(5′-CAYCCNGCHCGYGAYATGC-3′) and PheS-22-R (5′-CCWARVCCRAARGCAAARCC-3′). PCR product was checked by by electrophoresis on a 1% (wt/vol) agarose gel at 75 V for 45 min with marker (SmartLadder, Eurogentec, Searing, Belgium). PCR  product of DV4.10 collected a bold band at  450 bp position similar to size of pheS gene [6]. This PCR product continued to run the second PCR with seperated primer, purified, sequenced and comparated to sequencing of known sequence database in EMBL or BCCM/LMG collection of Gent university, Belgium. The result showed that  DV4.10 is Lactococcus lactis subsp. lactis species. Thus, the pheS gene sequence provides a useful tool. It have a higher discriminatory power for reliable identification of species level.

CÁC HỢP CHẤT PHENYLPROPANOID GLUCOSIDE TỪ CÂY BẠCH ĐỒNG NỮ CLERODENDRUM PHILIPINUM SCHAUER

SUMMARYFrom the methanolic extract of the roots of Clerodendrum philipinum four phenylpropanoid glucosides were isolated by various chromatography methods. Their structures were identified as clerodenoside A (1), martynoside (2), acteoside (3), and isoacteoside (4) by means of spectroscopic methods including 1D-, 2D-NMR, and MS in comparison with previous literature data. Compounds 2-4 were found for the first time from the Crolerodendrum species.Keywords: Clerodendrum philipinum, phenylpropanoid glucosideSUMMARYFrom the methanolic extract of the roots of Clerodendrum philipinum four phenylpropanoid glucosides were isolated by various chromatography methods. Their structures were identified as clerodenoside A (1), martynoside (2), acteoside (3), and isoacteoside (4) by means of spectroscopic methods including 1D-, 2D-NMR, and MS in comparison with previous literature data. Compounds 2-4 were found for the first time from the Crolerodendrum species.Keywords: Clerodendrum philipinum, phenylpropanoid glucosid

NGHIÊN CỨU CHẾ TẠO VÀ THỬ HOẠT TÍNH KHÁNG KHUẨN CỦA DUNG DỊCH NANO BẠC SỬ DỤNG CHITOSAN LÀM CHẤT KHỬ/CHẤT ỔN ĐỊNH

SUMMARY STUDY ON SYNTHESIS AND ANTIBACTERIAL ACTIVITY OF SILVER NANOPARTICLE CONTAINING SOLUTION USING CHITOSAN AS REDUCING/ STABILIZING REAGENT Silver nanoparticles (AgNPs) were synthesized using chitosan as reducing and stabilizing agent via a green and facile method. First, AgNO3 was mixed with chitosan solution, then, the mixture was heated up to 85 – 95oC for a cerain period of time (2 - 48 h), in which AgNPs were  formed and homogeniously distributed/penetrated inside chitosan chains. AgNPs were characterized by XRD, IR, TEM and UV-vis methods. The obtained results confirmed that AgNPs, whose the  particle size of about 7 - 12 nm, were coated by chitosan layer, therefore are stable for at least 4 months without any significant agglomeration. Antibacterial test of AgNPs were investigated, confirming their enhanced antibacterial activities thanks to synergic properties of both AgNPs and chitosan. Keywords. Silver nanoparticles, chitosan, antibacterial activit

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