3 research outputs found
Staphylococcal Peptidoglycan Co-Localizes with Nod2 and TLR2 and Activates Innate Immune Response via Both Receptors in Primary Murine Keratinocytes
- Author
- Publication venue
- Public Library of Science
- Publication date
- 07/10/2010
- Field of study
In mammalian host cells staphylococcal peptidoglycan (PGN) is recognized by Nod2. Whether PGN is also recognized by TLR2 is disputed. Here we carried out PGN co-localization and stimulation studies with TLR2 and Nod2 in wild type and mutant host cells. To exclude contamination with lipoproteins, polymeric staphylococcal PGN (PGNpol) was isolated from Staphylococcus aureus Δlgt (lacking lipidated prelipoproteins). PGNpol was biotinylated (PGN-Bio) for fluorescence monitoring with specific antibodies. Keratinocytes from murine oral epithelium (MK) readily internalized PGN-Bio in an endocytosis-like process. In wt MK, PGNpol induced intracellular accumulation of Nod2 and TLR2 and co-localized with Nod2 and TLR2, but not with TLR4. In TLR2-deficient MK Nod2 and in Nod2-deficient MK TLR2 was induced, indicating that PGNpol recognition by Nod2 is independent of TLR2 and vice versa. In both mutants IL-6 and IL-1B release was decreased by approximately 50% compared to wt MK, suggesting that the immune responses induced by Nod2 and TLR2 are comparable and that the two receptors act additively in MK. In TLR2-tranfected HEK293 cells PGNpol induced NFkB-promoter fused luciferase expression. To support the data, co-localization and signaling studies were carried out with SHL-PGN, a lipase protein covalently tethered to PGN-fragments of varying sizes at its C-terminus. SHL-PGN also co-localized with Nod2 or TLR2 and induced their accumulation, while SHL without PGN did not. The results show that staphylococcal PGN not only co-localizes with Nod2 but also with TLR2. PGN is able to stimulate the immune system via both receptors
Annuaire 2011-2012
- Author
- Abou-Zaki Hala
- Absi Pascale
- Abé Yoshio
- Abélès Marc
- Agier Michel
- Alaoui Narjys El
- Albert Jean-Pierre
- Albert-Llorca Marlène
- Alexandre-Bidon Danièle
- Alice Sarcinelli Sophie
- Alphandéry Pierre
- Amselle Jean-Loup
- Andezian Sossie
- Andreau Jean
- Anne Goldberg Sylvie
- Anstett Élisabeth
- Antoine Agnès
- Arar Tareq
- Archambault de Beaune Sophie
- Ariel de Vidas Anath
- Armatte Michel
- Arnoux Mathieu
- Arrizabalaga Marie-Pierre
- Arrondel Luc
- Artières Philippe
- Asséo Henriette
- Astafieva Elena
- Atlan Henri
- Atlani-Duault Laëtitia
- Aucante Yohann
- Audoin-Rouzeau Stéphane
- Aymard Maurice
- Aymes Marc
- Azria Régine
- Baciocchi Stéphane
- Backouche Isabelle
- Baldin Damien
- Balladur Catherine
- Barbe Noël
- Barbut Marc
- Barraud Cécile
- Barreyre Nicolas
- Barry John-Christopher
- Barry Laurent
- Barthélémy Michel
- Baruch Marc Olivier
- Baschet Jérôme
- Bataille Philippe
- Bataillon Gilles
- Battagliola Françoise
- Baumgarten Jean
- Baussant Michèle
- Beaune Sophie Archambault de
- Beauvallet Olivier
- Bellier Irène
- Belmont Nicole
- Benoist Jocelyn
- Bensa Alban
- Berelowitch Wladimir
- Berestycki Henri
- Bergeaud-Blackler Florence
- Berlivet Luc
- Bernard-Maugiron Nathalie
- Berra Aurélien
- Bert Jean-François
- Berthezène Clarisse
- Bertrand Michel
- Bessin Marc
- Beuvier Franck
- Bidet Alexandra
- Bigg Charlotte
- Bilbao Alejandro
- Blom Amélie
- Blum Alain
- Blundo Giorgio
- Blévis Laure
- Boi Luciano
- Boissinot Philippe
- Boleda Mario
- Bonin Pierre
- Bonnain-Dulon Rolande
- Bonneuil Noël
- Bonnin Michel
- Bontemps Véronique
- Bordes-Benayoun Chantal
- Bory Anne
- Bory Stefano
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- Bourdelais Patrice
- Bourdieu Jérôme
- Bourdonneau Éric
- Boureau Alain
- Bouretz Pierre
- Bourguignon François
- Bourmaud Philippe
- Boussouf Habib Idrissi
- Boutet Manuel
- Boutier Jean
- Boutry Philippe
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- Bouvier Yves
- Bozarslan Hamit
- Brac de la Perrière Bénédicte
- Bras Hervé Le
- Bras Jean-Philippe
- Breton Stéphane
- Bretschneider Falk
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- Choron-Baix Catherine
- Chouquer Gérard
- Ciambelli Patricia
- Cifoletti Giovanna C.
- Claire Élizabeth
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- Cloarec Jacques
- Clémentin-Ojha Catherine
- Coccia Emanuele
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- Cohen Yves
- Colleyn Jean-Paul
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- Demiaux Victor
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- Descola Philippe
- Descombes Vincent
- Desrosiers Sophie
- Devictor Agnès
- Dewerpe Alain
- De Almeida Mendes Antonio
- de Biase Alessia
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- Dhombres Jean
- Didi-Huberman Georges
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- Ducreux Marie-Élizabeth
- Ducrot Oswald
- Dudoignon Stéphane A.
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- Dumas Hélène
- Dumoulin David
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- Gaudillière Jean-Max
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- Gaulin Jean-Louis
- Gayet-Viaud Carole
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- Ingold Alice
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- Iteanu André
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- Jouhaud Christian
- Joukovskaïa Anna
- Joulian Frédéric
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- Judet de La Combe Pierre
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- Locher Fabien
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- Lyon-Caen Antoine
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- Mahé Alain
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- Noiriel Gérard
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- Obringer Frédéric
- Obrist Flans-Ulrich
- Odorico Paolo
- Ohayon Isabelle
- Olender Maurice
- Oliel-Grausz Évelyne
- Olivesi Vannina
- Olivier Laurent
- Olivier Baruch Marc
- Olivier de Sardan Jean-Pierre
- Orléan André
- Ortar Nathalie
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- Topalov Christian
- Topçu Sezin
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- Truc Gérôme
- Tsiomis Yannis
- Ullern Isabelle
- Urfalino Philippe
- Vancheri Luc
- Van De Velde Cécile
- Vapnarsky Valentina
- Varrel Aurélie
- Vasak Anouchka
- Vassas Claudine
- Vatchagaev Maïrbek
- Veinstein Gilles
- Veit Patrice
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- Venel Nancy
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- Verdier Thierry
- Vermeir Koen
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- Viala Alain
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- Vignemont Frédérique de
- Ville Isabelle
- Vincensini Jean-Jacques
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- Voix Raphaël
- Vratskidou Eleonora
- Wahnich Sophie
- Wang Simeng
- Weil François
- Werner Michael
- Wiesbein Julien
- Wieviorka Michel
- Will Pierre-Étienne
- Wismann Heinz
- Wittersheim Éric
- Worms Frédéric
- Xiao-Planes Xiaohong
- Yacine Tassadit
- Yatabe Kazuhiko
- Zakharova Larissa
- Zalewski Frédéric
- Zancarini-Fournel Michelle
- Zander Ulrike
- Zarcone Thierry
- Zhuravskaya Ekaterina
- Zimmermann Bénédicte
- Zimmermann Francis
- Zurbach Julien
- Zuñiga Jean-Paul
- Zytnicki Colette
- Élie Marc
- Özkoray Hayri Göksin
- Županov Ines
- Publication venue
- Annuaire de l'EHESS
- Publication date
- 23/09/2015
- Field of study