A Disruptive Educational Project in Secondary Education. The Case of IES Cartagines.

The project presented here arises from the new educational needs that emanate from the changes taking place in the social, cultural, political, and economic spheres. The boundaries of formal education, as it has been understood until now, are being breached by new forms of learning, communication, and relationships, which are claiming their place in educational institutions. This is particularly relevant at the level of secondary education where the boundaries of conventional cultural and social systems are constantly being overwhelmed by these new realities (Fernández Enguita, 2016). This makes it necessary to rethink the systems of teaching and working in the educational system. Students at this educational level have a profile and resources closely linked to the knowmadic society (Cobo, 2016, Movarec, 2008), in which there is less and less sense in a teaching model based mainly on the unidirectional transmission of knowledge (Downes, 2017). This, in interaction with families, teachers and other educational agents, generates a world of conflicts of various kinds: curricular, social, attitudinal and expectations, etc.Ministerio de Economía y competitividad. RT2018-097144-b- 100 Uma20-FEDERIA-121 Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech

Regulation and symbiotic significance of nodulation outer proteins secretion in Sinorhizobium fredii HH103

In this work we show that the Sinorhizobium fredii HH103 ttsI gene is essential for the expression of the tts genes and secretion of nodulation outer proteins (Nops). Moreover, we demonstrate for the first time, to our knowledge, that the nod box preceding ttsI is necessary for Nops secretion. TtsI is responsible for the transcriptional activation of nopX, nopA, rhcJ and rhcQ. We confirm that the S. fredii HH103 ttsI gene is activated by NodD1 and repressed by NolR. In contrast, NodD2 is not involved in the regulation of ttsI expression. Despite the dependence of expression of both ttsI and nodA on NodD1 and flavonoids, clear differences in the capacity of some flavonoids to activate these genes were found. The expression of the ttsI and nodA genes was also sensitive to differences in the pH of the media. Secretion of Nops in the ttsI mutant could not be complemented with a DNA fragment containing the ttsI gene and its nod box, but it was restored when a plasmid harbouring the ttsI, rhcC2 and y4xK genes was transferred to the mutant strain. The symbiotic effect of Nops secretion was host-dependent but independent of the type of nodule formed by the host legume. Nops are beneficial in the symbiosis with Glycine max and Glycyrrhiza uralensis, and detrimental in the case of the tropical legume Erythrina variegata

Investigación sobre la formulación y elaboración de néctar con cardamomo

Para la elaboración de un néctar de naranja con cardamomo se partió del hecho que se quería otorgar un uso al cardamomo nacional, proponiendo una alternativa en su procesamiento. Para ello se combinó con naranja, debido a la afinidad de dicha semilla con los cítricos, para producir un néctar de naranja con cardamomo. Se inició con tres procesos de estandarización los cuales fueron el cardamomo, la cantidad de azúcar en el néctar y la estandarización del tipo de naranja a utilizar. Esto para cumplir con las regulaciones establecidas para un néctar de naranja, tanto de pH como de °Brix, según el RTCA “Alimentos y bebidas procesados. Néctares de frutas. Especificaciones”, así como para que el néctar elaborado cumpliera con las propiedades organolépticas que el consumidor busca, las cuales fueron medidas por medio de la evaluación sensorial realizada a 75 personas. Para realizar el proceso de estandarización de la cantidad de cardamomo se realizaron infusiones a tres concentraciones de cardamomo a 25%, 33% y 50%. El fin para determinar cuál de las infusiones guardaba mejor el aroma a cardamomo y además cual no dejaba un regusto indeseado o un regusto muy fuerte a cardamomo, además de determinar cuál de las tres tenía mejor armonía con el jugo de la naranja al producir el néctar. Posteriormente, ya realizada la estandarización de la cantidad de cardamomo, correspondiente al 33%, se realizó el proceso de estandarización de la cantidad de sacarosa. Para realizar esto, al igual que con el cardamomo, se utilizaron tres concentraciones diferentes correspondientes al 10%, 20% y 25% de sacarosa en solución. El fin de estandarizar en nivel o concentración de sacarosa fue obtener el nivel de °Brix requerido para el néctar de naranja con cardamomo el cual según el RTCA 67.04.48:08 el cual es de 11.2°Brix. Por último, al igual que con el nivel de cardamomo y sacarosa se realizó la estandarización del tipo de naranja a utilizar, la cual debía favorecer el cumplimiento con parámetros de °Brix y pH según el RTCA. La naranja utilizada luego de realizadas las pruebas fue la naranja de tipo Valenciana ya que presentó condiciones de pH y °Brix aceptables para cumplir con el RTCA correspondiente, a diferencia de otras pruebas que se realizaron con naranjas lisas salustianas. Ya con la formulación establecida y el néctar elaborado cumpliendo parámetros de °Brix y pH según RTCA se procedió a realizar una evaluación sensorial para ver el grado de aceptación del producto para el consumidor. El costo total para la elaboración del néctar de naranja con cardamomo fue de Q15.99 con un rendimiento promedio de las naranjas de 51%

A pyrF auxotrophic mutant of Sinorhizobium fredii HH103 impaired in its symbiotic interaction with soybean and other legumes

Transposon Tn5-Mob mutagenesis allowed the selection of a Sinorhizobium fredii HH103 mutant derivative (SVQ 292) that requires the presence of uracil to grow in minimal media. The mutated gene, pyrF, codes for an orotidine-5 ́- monophosphate decarboxylase (EC 4.1.1.23). Mutant SVQ 292 and its parental prototrophic mutant HH103 showed similar Nod-factor and lipopolysaccharide profiles. The symbiotic properties of mutant SVQ 292 were severely impaired with all legumes tested. Mutant SVQ 292 formed small ineffective nodules on Cajanus cajan and abnormal nodules (pseudonodules) unable to fix nitrogen on Glycine max (soybean), Macroptitlium atropurpureum, Indigofera tinctoria, and Desmodium cana-dense. It also did not induce any macroscopic response in Macrotyloma axillare roots. The symbiotic capacity of SVQ 292 with soybean was not enhanced by the addition of uracil to the plant nutritive solution

Retrieval of germinal zone neural stem cells from the cerebrospinal fluid of premature infants with intraventricular hemorrhage

Intraventricular hemorrhage is a common cause of morbidity and mortality in premature infants. The rupture of the germinal zone into the ventricles entails loss of neural stem cells and disturbs the normal cytoarchitecture of the region, compromising late neurogliogenesis. Here we demonstrate that neural stem cells can be easily and robustly isolated from the hemorrhagic cerebrospinal fluid obtained during therapeutic neuroendoscopic lavage in preterm infants with severe intraventricular hemorrhage. Our analyses demonstrate that these neural stem cells, although similar to human fetal cell lines, display distinctive hallmarks related to their regional and developmental origin in the germinal zone of the ventral forebrain, the ganglionic eminences that give rise to interneurons and oligodendrocytes. These cells can be expanded, cryopreserved, and differentiated in vitro and in vivo in the brain of nude mice and show no sign of tumoral transformation 6 months after transplantation. This novel class of neural stem cells poses no ethical concerns, as the fluid is usually discarded, and could be useful for the development of an autologous therapy for preterm infants, aiming to restore late neurogliogenesis and attenuate neurocognitive deficits. Furthermore, these cells represent a valuable tool for the study of the final stages of human brain development and germinal zone biology

Polarimetric imaging for the detection of synthetic models of SARS-CoV-2: A proof of concept

Objective: To conduct a proof-of-concept study of the detection of two synthetic models of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) using polarimetric imaging. Approach: Two SARS-CoV-2 models were prepared as engineered lentiviruses pseudotyped with the G protein of the vesicular stomatitis virus, and with the characteristic Spike protein of SARS-CoV-2. Samples were prepared in two biofluids (saline solution and artificial saliva), in four concentrations, and deposited as 5-µL droplets on a supporting plate. The angles of maximal degree of linear polarization (DLP) of light diffusely scattered from dry residues were determined using Mueller polarimetry from87 samples at 405 nm and 514 nm. A polarimetric camera was used for imaging several samples under 380–420 nm illumination at angles similar to those of maximal DLP. Per-pixel image analysis included quantification and combination of polarization feature descriptors in 475 samples. Main results: The angles (from sample surface) of maximal DLP were 3° for 405 nm and 6° for 514 nm. Similar viral particles that differed only in the characteristic spike protein of the SARS-CoV-2, their corresponding negative controls, fluids, and the sample holder were discerned at 10-degree and 15-degree configurations. Significance: Polarimetric imaging in the visible spectrum may help improve fast, non-contact detection and identification of viral particles, and/or other microbes such as tuberculosis, in multiple dry fluid samples simultaneously, particularly when combined with other imaging modalities. Further analysis including realistic concentrations of real SARS-CoV-2 viral particles in relevant human fluids is required. Polarimetric imaging under visible light may contribute to a fast, cost-effective screening of SARS-CoV-2 and other pathogens when combined with other imaging modalities.12 página

Hyperspectral image processing for the identification and quantification of lentiviral particles in fluid samples

Optical spectroscopic techniques have been commonly used to detect the presence of biofilm-forming pathogens (bacteria and fungi) in the agro-food industry. Recently, near-infrared (NIR) spectroscopy revealed that it is also possible to detect the presence of viruses in animal and vegetal tissues. Here we report a platform based on visible and NIR (VNIR) hyperspectral imaging for non-contact, reagent free detection and quantification of laboratory-engineered viral particles in fluid samples (liquid droplets and dry residue) using both partial least square-discriminant analysis and artificial feed-forward neural networks. The detection was successfully achieved in preparations of phosphate buffered solution and artificial saliva, with an equivalent pixel volume of 4 nL and lowest concentration of 800 TU.mu L-1. This method constitutes an innovative approach that could be potentially used at point of care for rapid mass screening of viral infectious diseases and monitoring of the SARS-CoV- 2 pandemic.This research was funded by grants number COV20-00080 and COV20-00173 of the 2020 Emergency Call for Research Projects about the SARS-CoV-2 virus and the COVID-19 disease of the Institute of Health 'Carlos III', Spanish Ministry of Science and Innovation, and by grant number EQC2019-006240-P of the 2019 Call for Acquisition of Scientific Equipment, FEDER Program, Spanish Ministry of Science and Innovation. This work has been supported by the European Commission through the JRC HUMAINT project. ABR was supported by grant number RTI2018-094465-J funded by the Spanish National Agency of Research. The authors would like to gratefully acknowledge the assistance of the members of the EOD-CBRN Group of the Spanish National Police, whose identities cannot be disclosed, and who are represented here by JMNG. Authors thank continuous support from their institutions