Circulating MicroRNAs as Potential Biomarkers of Exercise Response

Systematic physical activity increases physical fitness and exercise capacity that lead to the improvement of health status and athletic performance. Considerable effort is devoted to identifying new biomarkers capable of evaluating exercise performance capacity and progress in training, early detection of overtraining, and monitoring health-related adaptation changes. Recent advances in OMICS technologies have opened new opportunities in the detection of genetic, epigenetic and transcriptomic biomarkers. Very promising are mainly small non-coding microRNAs (miRNAs). miRNAs post-transcriptionally regulate gene expression by binding to mRNA and causing its degradation or inhibiting translation. A growing body of evidence suggests that miRNAs affect many processes and play a crucial role not only in cell differentiation, proliferation and apoptosis, but also affect extracellular matrix composition and maintaining processes of homeostasis. A number of studies have shown changes in distribution profiles of circulating miRNAs (c-miRNAs) associated with various diseases and disorders as well as in samples taken under physiological conditions such as pregnancy or physical exercise. This overview aims to summarize the current knowledge related to the response of blood c-miRNAs profiles to different modes of exercise and to highlight their potential application as a novel class of biomarkers of physical performance capacity and training adaptation

Vertical jump development in elite adolescent volleyball players: Effects of sex and age

Background: Vertical jump is one of the most prevalent activities performed in volleyball. During adolescence, boys and girls undergo substantial changes in physiological and anthropometric characteristics that influence vertical jump performance. Objective: The purpose of this study was to examine and quantify sex and age-related differences in vertical jump performance assessed as vertical jumps height (VJH) and ground contact time (Tc) during repeated vertical jump tests within the Czech and Slovak population of elite adolescent volleyball players. Further aims were to compare the obtained results to non-athletic adolescent national normative data and investigate the relationship between basic anthropometric measures and VJH. Methods: The cross-sectional study was conducted on 203 females and 180 males from 13 to 19 years old. Statistical analysis of VJH differences between sex and age categories was performed using two-way ANOVA with Tukey's post hoc test. The relationship between basic anthropometric parameters and VJH were examined by Pearson's correlation analysis. Results: ANOVA showed statistically significant differences in VJH between males and females (p .05). Volleyball players have significantly higher VJH than non-athletes across all age categories and sex differences in volleyball players were smaller than the sex differences among non-athletic adolescents. A correlation analysis revealed only weak sex specific correlations of VJH with body height, body mass and BMI. Conclusions: The presented study showed significant sex differences in VJH between elite male and female volleyball players from the age of 15. VJH increases continually with increasing age in males. In females the effect of age on VJH was insignificant

Circulating MicroRNAs as Potential Biomarkers of Exercise Response

Systematic physical activity increases physical fitness and exercise capacity that lead to the improvement of health status and athletic performance. Considerable effort is devoted to identifying new biomarkers capable of evaluating exercise performance capacity and progress in training, early detection of overtraining, and monitoring health-related adaptation changes. Recent advances in OMICS technologies have opened new opportunities in the detection of genetic, epigenetic and transcriptomic biomarkers. Very promising are mainly small non-coding microRNAs (miRNAs). miRNAs post-transcriptionally regulate gene expression by binding to mRNA and causing its degradation or inhibiting translation. A growing body of evidence suggests that miRNAs affect many processes and play a crucial role not only in cell differentiation, proliferation and apoptosis, but also affect extracellular matrix composition and maintaining processes of homeostasis. A number of studies have shown changes in distribution profiles of circulating miRNAs (c-miRNAs) associated with various diseases and disorders as well as in samples taken under physiological conditions such as pregnancy or physical exercise. This overview aims to summarize the current knowledge related to the response of blood c-miRNAs profiles to different modes of exercise and to highlight their potential application as a novel class of biomarkers of physical performance capacity and training adaptation

Self-Assembly Properties of Cationic Gemini Surfactants with Biodegradable Groups in the Spacer

: Self-assembly properties of cationic gemini surfactants with biodegradable amide or ester groups in the spacer were investigated utilising time-resolved fluorescence quenching, dynamic light scattering and zeta potential measurements. A correlation between aggregation parameters such as micelle aggregation number, micelle size and zeta potential with the structure of gemini molecules was made. For gemini molecules with medium spacer lengths, micelle aggregation number does not change much with the surfactant concentration. When the spacer is extended, a stronger aggregation tendency is observed for gemini surfactant molecules with two ester groups in the spacer and the aggregation number increases. The assumption of stronger aggregation of ester-based gemini molecules at larger spacer number values is also documented by measurements of the size and zeta potential of ester-based micelles. The explanation of the difference in aggregation ability of amide-based and ester-based gemini molecules is related to the structural features of gemini molecules, notably to the larger flexibility and denser arrangement of ester-based gemini molecules in a micelle. To support this assumption, optimised 3D models of the studied gemini molecules were constructed. Correspondingly, the calculations show smaller size and interfacial area for ester-based gemini conformers