Preparation of “Open/closed” pores of PLGA-microsphere for controlled release of protein drug

Poly(D,L-lactic-co-glycolic acid)  has been extensively used as a controlled release carrier for drug delivery due to its good biocompatibility, biodegradability, and mechanical strength. In this study, porous PLGA microspheres were fabricated by an emulsion-solvent evaporation technique using poly ethylene glycol (PEG) as an extractable porogen and loaded with  protein (lysozyme) by suspending them in protein solution. For controlled release of protein, porous microspheres containing lysozyme were treated with water-miscible solvents in aqueous phase for production of pore-closed microspheres. The surface morphology of microspheres were investigated using scanning electron microscopy (SEM) for confirmation of its porous microstructure structure. Protein property after release was observed by enzymatic activity assay. The pore-closing process resulted in nonporous microspheres which exhibited sustained release patterns over an extended period

Utilization of spent brewer’s yeast Saccharomyces cerevisiae for the production of yeast enzymatic hydrolysate

Spent brewer’s yeast (Saccharomyces cerevisiae) is a rich source of protein, vitamins and widely used as a raw material for production of food supplements. The autolysis and enzymatic treatment of spent brewer’s yeast using Pancreatin (2.5%) and Flavourzyme (2.5%) were performed at 45 °C and 50 °C, respectively. The autolysis and hydrolysis processes were evaluated by determining a soluble solids, soluble protein concentration and α-amino nitrogen content in a reaction mixture. The yield of pancreatic digest and α-amino nitrogen content was high in comparison with autolysis and Flavourzyme treatment. The total solids recovery in dry Yeast hydrolysate was about 50%, a protein and α-amino nitrogen content was 55.9 and 4.8%, respectively. These results show the possibility of utilizing the spent brewer’s yeast as hydrolysate using hydrolytic enzymes and use it as a food supplement after biological experiments.DOI: http://dx.doi.org/10.5564/mjc.v12i0.179 Mongolian Journal of Chemistry Vol.12 2011: 88-9

A field study evaluating the humoral immune response in Mongolian sheep vaccinated against sheeppox virus

Sheeppox is a transboundary disease of sheep caused by infection with the capripoxvirus sheeppox virus (SPPV). Sheeppox is found in Africa, the Middle East and Asia and is characterised by fever, multifocal cutaneous raised lesions, and death, with substantial negative impact on affected flocks. Vaccination with live attenuated capripoxvirus (CPPV) strains is an effective and widely used means of controlling sheeppox outbreaks, however there are few reports of post-vaccination field surveillance studies of sheeppox. This study used a commercially available ELISA and a fluorescence-based neutralisation assay (FVNT) to examine quantitative and temporal features of the humoral response of sheep vaccinated with a live attenuated CPPV strain in Mongolia. 400 samples were tested using the ELISA, and a subset of 45 also tested with the FVNT. There was substantial agreement between the FVNT and ELISA tests. Antibodies to CPPV were detected between 40 and 262 days post vaccination. There was no significant difference between serological status (positive / negative) and sex or age, however an inverse correlation was found between the length of time since vaccination and serological status. Animals between 90 and 180 days post-vaccination were more likely to be positive than animals greater than 180 days post vaccination. This data provides temporal parameters to consider when planning sheeppox post-vaccination monitoring programmes. In summary, our results show a commercial CPPV ELISA kit is a robust and reliable assay for use in resource-restricted low and low-middle income countries for post CPPV vaccination surveillance on a regional or national level.The attached .xls file contains all raw data used in the associated publication, " A comparative serological field study evaluating the humoral immune response in Mongolian sheep vaccinated against sheeppox virus." The dataset identifies all individual sheep by a unique identification number (ID_num). Each unique ID_num is associated with relevant metadata: Province name, Sum name, herder name coded (Herded_Id), animal species, age of the animal, in years, when sampled (Age), sex of the animal "F" or "M" (Sex), date when the animal was sampled (Date_sample_collected), date when the animal was vaccinated for sheeppox according to the vaccination records (Date_vaccinated_raw data), and for samples in which the exact date of vaccination was not available and a range of potential time was given, the midpoint date within this range (Date_vaccinated_midpoint), animals for which vaccination date was not available receive an "NA" value; time from vaccination to sampled in days (Time_from_vaccinated_midpoint); %S/P values for the ELISA test conducted in the Mongolia lab (Ag_ELISA_SCVL_OD_SP) and %S/P values for the ELISA test conducted in the UK lab (Ag_ELISA_TPI_OD_SP), results from the ELISA test classified as binary variable "Positive" or "Negative" (Ag_ELISA_SCVL_bin and Ag_ELISA_TPI_bin); titres from the fluorescence-based neutralisation assay (FVNT Titre) and results from the FVNT test classified as binary variable ("Positive" or "Negative"), all animals that were not tested by FVNT receive an "NA" value in these columns. Funding provided by: Biotechnology and Biological Sciences Research CouncilCrossref Funder Registry ID: http://dx.doi.org/10.13039/501100000268Award Number: BBS/E/I/00007031Funding provided by: Biotechnology and Biological Sciences Research CouncilCrossref Funder Registry ID: http://dx.doi.org/10.13039/501100000268Award Number: BB/E/I/00007036Funding provided by: Biotechnology and Biological Sciences Research CouncilCrossref Funder Registry ID: http://dx.doi.org/10.13039/501100000268Award Number: BB/E/I/00007037Funding provided by: Biotechnology and Biological Sciences Research CouncilCrossref Funder Registry ID: http://dx.doi.org/10.13039/501100000268Award Number: BBS/E/I/00007039Funding provided by: Biotechnology and Biological Sciences Research CouncilCrossref Funder Registry ID: http://dx.doi.org/10.13039/501100000268Award Number: BB/J004324/1Funding provided by: Biotechnology and Biological Sciences Research CouncilCrossref Funder Registry ID: http://dx.doi.org/10.13039/501100000268Award Number: BBS/E/D/20002173Funding provided by: Horizon 2020Crossref Funder Registry ID: http://dx.doi.org/10.13039/501100007601Award Number: 773701Funding provided by: Food and Agriculture Organization of the United Nations*Crossref Funder Registry ID: Award Number: TCP/MON/3603Funding provided by: IdVet*Crossref Funder Registry ID: Award Number: Funding provided by: Food and Agriculture Organization of the United NationsCrossref Funder Registry ID: Award Number: TCP/MON/3603Funding provided by: IdVetCrossref Funder Registry ID:Blood samples from sheep and associated data were collected as part of the post-vaccination surveillance programme for sheeppox implemented by the Mongolian General Authority for Veterinary Services (GAVS) in 2016