TOTAL PHENOL, FLAVONOID AND ANTIOXIDANT PROPERTIES OF AURICULARIA AURICULA-JUDAE

Objective: From ancient times, Auricularia auricula has been used as food and also as herbal medicine in China. The hot water extract of Auricularia auricula fruiting bodies were evaluated for free radical scavenging potency, total phenol and flavonoid content.Methods: The antioxidant property was investigated using different in vitro assays such as, ABTS (2,2'-azino-bis[3-ethylbenzothiazoline-6-sulphonicacid]), DMPD (Dimethyl-4-phenylenediamine) radical scavenging assay, reducing power, phenanthroline assay, lipid peroxidation and erythrocyte hemolysis inhibition assays and also subjected to analyse total phenol, flavonoid levels and total antioxidant capacity by phospho- molybdenum assay.Results: The levels of total phenol and flavonoid were found to be 8.94 mg CE/g, 3.49 mg RE/g respectively. The EC50 values are<10 mg/ml in all the assays which indicate its strong scavenging activities. The antioxidant activity was found to be concentration-dependent. A significant difference (P<0.05) was observed between the concentrations tested.Conclusion: These results suggest that Auricularia auricula mushrooms are good scavengers of ABTS, DMPD radicals, reducer of ferric ions, and inhibitor of lipid peroxidation and erythrocyte hemolysis. Thus, it could potentially be used as a source of bioactive compounds.Â

BIOACTIVE CONSTITUENTS AND ANTIOXIDANT EFFICACY OF AURICULARIA POLYTRICHA

Objective: Auricularia polytricha is a most popular edible and medicinal fungus in China and other Asian countries because of its high nutritionalvalue and good taste. The objective of this study was to evaluate the antioxidant potency, total phenol, and flavonoid content in the hot water extractof A. polytricha fruiting bodies.Methods: The antioxidant property was investigated using various in vitro chemical and biological models such as 2, 2'-azino-bis [3-ethylbenzothiazoline6-sulfonicacid],dimethyl-4-phenylenediamineradicalscavengingassay,cupric reducingantioxidantcapacityassay,phosphomolybdenumreducingantioxidantpowerassay,β-carotenebleaching and lipid peroxidationinhibition assays.Results: A. polytricha exhibited antioxidant activity in a dose-dependent manner with lower ECvalues in all scavenging assays. A considerableamount of secondary metabolites such as phenol and flavonoids were observed in the extract.50 Conclusion: The results demonstrated that A. polytricha has a potential significance for seeking new natural antioxidant agents against oxidativestress.Keywords: β-carotene bleaching, Free radicals scavenging, Lipid peroxidation, Total phenols, Total flavonoids, Wood ear mushrooms

ANTIOXIDANT ACTIVITY AND BIOACTIVE CONSTITUENTS IN THE FRUITING BODIES OF HERICUM ERINACEUS PERS, AN EDIBLE MUSHROOM

Objectives: Hericium is a genus of mushrooms in the Hericiaceae family. Hericium erinaceus has been used for the treatment of various diseases for over 2000 y in China. The study investigated the bioactive components and antioxidant properties of the hot water extract from the fruiting body of H. erinaceus.Methods: The hot water extract of H. erinaceus was estimated for antioxidant potency using different analysis, such as 1,1-diphenyl-2-picryl-hydrazyl (DPPH) radical scavenging assay, ferric reducing antioxidant power (FRAP) assay, cupric ions reducing antioxidant capacity (CUPRAC) assay, metal ion chelation, erythrocyte hemolysis inhibition assay and compared with the reference standards. Total phenolic, flavonoid contents, and total antioxidant capacity were also determined using spectrophotometric methods.Results: H. erinaceus showed concentration dependent activity in all the assays with following EC50 values. DPPH (EC50= 2.13 mg/ml), FRAP (EC50= 7.57 mg/ml), CUPRAC (EC50= 0.97 mg/ml), ferrous ion chelation (EC50= 2.31 mg/ml) and erythrocytes hemolysis inhibition (EC50= 4.88 mg/ml). The total phenolic and flavonoid content in H. erinaceus was found to be 8.77 mg GAE/g and 5.62 mg RE/g respectively.Conclusion: It was concluded that the hot water extract of the H. erinaceus exhibit potent free radical scavenging activity and can be used as natural antioxidants owing to their significant antioxidant activity.Keywords: Antioxidant, Scavenging activity, Free radicals, Reducing power, Hericium erinaceus pers, Total flavonoids, Total phenolic