Bioturbation by Hexagenia larvae (Ephemeroptera: Ephemeridae) and its implications for sediment suspension in Lake Erie.

Burrowing activities of aquatic benthic organisms can influence sediment suspension and nutrient cycling. The burrowing mayfly Hexagenia is a dominant benthic organism in western Lake Erie, and Hexagenia bioturbation can potentially influence water quality and other benthic organisms. Using laboratory experiments, I determined bioturbation-induced sediment suspension rates of fine Lake Erie sediment caused by Hexagenia larvae of varying body lengths (13--28 mm) at densities of 70--1,111 larvae/m2 and water temperatures ranging from 10--25°C. Bioturbation rates (sediment suspension, g/m2/h) were estimated by nonlinear regression from measurements of suspended sediment concentrations in jars, taken twice-daily for 14 d. Sediment settling rates were also estimated from twice-daily measurements of suspended sediment concentrations collected for an additional 18 d in the surface water from the bioturbation experiments. (Abstract shortened by UMI.) Source: Masters Abstracts International, Volume: 43-01, page: 0145. Adviser: Jan J. H. Ciborowski. Thesis (M.Sc.)--University of Windsor (Canada), 2004

A mantle plume origin for the Palaeoproterozoic Circum-Superior Large Igneous Province

The Circum-Superior Large Igneous Province (LIP) consists predominantly of ultramafic-mafic lavas and sills with minor felsic components, distributed as various segments along the margins of the Superior Province craton. Ultramafic-mafic dykes and carbonatite complexes of the LIP also intrude the more central parts of the craton. Most of this magmatism occurred ∼1880 Ma. Previously a wide range of models have been proposed for the different segments of the CSLIP with the upper mantle as the source of magmatism. New major and trace element and Nd-Hf isotopic data reveal that the segments of the CSLIP can be treated as a single entity formed in a single tectonomagmatic environment. In contrast to most previous studies that have proposed a variety of geodynamic settings, the CSLIP is interpreted to have formed from a single mantle plume. Such an origin is consistent with the high MgO and Ni contents of the magmatic rocks, trace element signatures that similar to oceanic-plateaus and ocean island basalts and εNd-εHf isotopic signatures which are each more negative than those of the estimated depleted upper mantle at ∼1880 Ma. Further support for a mantle plume origin comes from calculated high degrees of partial melting, mantle potential temperatures significantly greater than estimated ambient Proterozoic mantle and the presence of a radiating dyke swarm. The location of most of the magmatic rocks along the Superior Province margins probably represents the deflection of plume material by the thick cratonic keel towards regions of thinner lithosphere at the craton margins. The primary magmas, generated by melting of the heterogeneous plume head, fractionated in magma chambers within the crust, and assimilated varying amounts of crustal material in the process

Local and exotic sources of sarsen debitage at Stonehenge revealed by geochemical provenancing

The application of novel geochemical provenancing techniques has changed our understanding of the construction of Stonehenge, by identifying West Woods on the Marlborough Downs as the likely source area for the majority of the extant sarsen megaliths at the monument. In this study, we apply the same techniques to saccharoid sarsen fragments from three excavations within and outwith the main Sarsen Circle to expand our understanding of the provenance of sarsen debitage present at the monument. Through pXRF analysis, we demonstrate that the surface geochemistry of 1,028 excavated sarsen fragments is significantly affected by subsurface weathering following burial in a way that cannot be overcome by simple cleaning. However, we show that this effect is surficial and does not have a volumetrically significant impact, thus permitting the subsequent use of whole-rock analytical methods. Comparison of ICP-AES and ICP-MS trace element data from 54 representative sarsen fragments with equivalent data from Stone 58 at Stonehenge demonstrates that none are debitage produced during the dressing of this megalith or its 49 chemical equivalents at the monument. Further inspection of the ICP-MS data reveals that 22 of these fragments fall into three distinct geochemical ‘families’. None of these families overlap with the geochemical signature of Stone 58 and its chemical equivalents, implying that sarsen imported from at least a further three locations (in addition to West Woods) is present at Stonehenge. Comparison of immobile trace element signatures from the 54 excavated sarsen fragments against equivalent data for 20 sarsen outcrop areas across southern Britain shows that 15 of the fragments can be linked to specific localities. Eleven of these were likely sourced from Monkton Down, Totterdown Wood and West Woods on the Marlborough Downs (25–33 km north of Stonehenge). Three fragments likely came from Bramdean, Hampshire (51 km southeast of Stonehenge), and one from Stoney Wish, East Sussex (123 km to the southeast). Technological analysis and refitting shows that one of the fragments sourced from Monkton Down was part of a 25.7 cm × 17.9 cm flake removed from the outer surface of a large sarsen boulder, most probably during on-site dressing. This adds a second likely source area for the sarsen megaliths at Stonehenge in addition to West Woods. At this stage, we can only speculate on why sarsen from such diverse sources is present at Stonehenge. We do not know whether the fragments analysed by ICP-MS were removed from (i) the outer surface of Stones 26 or 160 (which are chemically distinct to the other extant sarsen megaliths), (ii) one of the c.28 sarsen megaliths and lintels from the c.60 erected during Stage 2 of the construction of Stonehenge that may now be missing from the monument, or (iii) one of the dismantled and destroyed sarsen megaliths associated with Stage 1 of the monument. With the exception of the fragment sourced from Monkton Down, it is also possible that the analysed fragments were (iv) pieces of saccharoid sarsen hammerstones or their pre-forms, or (v) small blocks brought on-site for ceremonial or non-ceremonial purposes

Proteomic Analysis of HIV-Infected Macrophages

Mononuclear phagocytes (monocytes, macrophages, and microglia) play an important role in innate immunity against pathogens including HIV. These cells are also important viral reservoirs in the central nervous system and secrete inflammatory mediators and toxins that affect the tissue environment and function of surrounding cells. In the era of antiretroviral therapy, there are fewer of these inflammatory mediators. Proteomic approaches including surface enhancement laser desorption ionization, one- and two-dimensional difference in gel electrophoresis, and liquid chromatography tandem mass spectrometry have been used to uncover the proteins produced by in vitro HIV-infected monocytes, macrophages, and microglia. These approaches have advanced the understanding of novel mechanisms for HIV replication and neuronal damage. They have also been used in tissue macrophages that restrict HIV replication to understand the mechanisms of restriction for future therapies. In this review, we summarize the proteomic studies on HIV-infected mononuclear phagocytes and discuss other recent proteomic approaches that are starting to be applied to this field. As proteomic instruments and methods evolve to become more sensitive and quantitative, future studies are likely to identify more proteins that can be targeted for diagnosis or therapy and to uncover novel disease mechanisms

Environmental Indicators for the Coastal Region of the U.S. Great Lakes

The goal of this research collaboration was to develop indicators that both estimate environmental condition and suggest plausible causes of ecosystem degradation in the coastal region of the U.S. Great Lakes. The collaboration consisted of 8 broad components, each of which generated different types of environmental responses and characteristics of the coastal region. These indicators included biotic communities of amphibians, birds, diatoms, fish, macroinvertebrates, and wetland plants as well as indicators of polycyclic aromatic hydrocarbon (PAH) photo-induced toxicity and landscape characterization. These components are summarized below and discussed in more detailed in 5 separate reports (Section II). Stress gradients within the U.S. Great Lakes coastal region were defined from 207 variables (e.g., agriculture, atmospheric deposition, land use/land cover, human populations, point source pollution, and shoreline modification) from 19 different data sources that were publicly available for the coastal region. Biotic communities along these gradients were sampled with a stratified, random design among representative ecosystems within the coastal zone. To achieve the sampling across this massive area, the coastal region was subdivided into 2 major ecological provinces and further subdivided into 762 segment sheds. Stress gradients were defined for the major categories of human-induced disturbance in the coastal region and an overall stress index was calculated which represented a combination of all the stress gradients. Investigators of this collaboration have had extensive interactions with the Great Lakes community. For instance, the Lake Erie Lakewide Area Management Plan (LAMP) has adopted many of the stressor measures as integral indicators of the condition of watersheds tributary to Lake Erie. Furthermore, the conceptual approach and applications for development of a generalized stressor gradient have been incorporated into a document defining the tiered aquatic life criteria for defining biological integrity of the nation’s waters. A total of 14 indicators of the U.S. Great Lakes coastal region are presented for potential application. Each indicator is summarized with respect to its use, methodology, spatial context, and diagnosis capability. In general, the results indicate that stress related to agricultural activity and human population density/development had the largest impacts on the biotic community indicators. In contrast, the photoinduced PAH indicator was primarily related to industrial activity in the U.S. Great Lakes, and over half of the sites sampled were potentially at risk of PAH toxicity to larval fish. One of the indicators developed for land use/land change was developed from Landsat imagery for the entire U.S. Great Lakes basin and for the period from 1992 to 2001. This indicator quantified the extensive conversions of both agricultural and forest land to residential area that has occurred during a short 9 year period. Considerable variation in the responses were manifest at different spatial scales and many at surprisingly large scales. Significant advances were made with respect to development of methods for identifying and testing environmental indicators. In addition, many indicators and concepts developed from this project are being incorporated into management plans and U.S. 8 EPA methods documents. Further details, downloadable documents, and updates on these indicators can be found at the GLEI website - http://glei.nrri.umn.edu