2 research outputs found
ΠΠΎΠ²ΡΡΠ΅Π½Π½ΡΠ΅ ΡΠ΅ΠΌΠΏΠ΅ΡΠ°ΡΡΡΡ Π²ΡΠ·ΡΠ²Π°ΡΡ ΠΎΠ±ΡΠ°Π·ΠΎΠ²Π°Π½ΠΈΠ΅ ΠΠ€Π ΠΈ Π½Π°ΡΡΡΠ΅Π½ΠΈΡ Π΄ΡΡ Π°Π½ΠΈΡ Π² ΠΊΠ»Π΅ΡΠΊΠ°Ρ ΡΡΡΠΏΠ΅Π½Π·ΠΈΠΎΠ½Π½ΠΎΠΉ ΠΊΡΠ»ΡΡΡΡΡ Saccharum officinarum
High temperatures are important abiotic stressors affecting plant growth, development and productivity. One of the consequences of unfavourable temperature effects on plants is an increase in reactive oxygen species (ROS) generation. However, what role ROS will play in the further fate of the cell under temperature stress depends on many external and internal factors. Therefore, the aim of this study was to identify the relationship between ROS content and mitochondrial function in the cells of a Saccharum officinarum suspension culture under high temperatures. The work was carried out using fluorescence microscopy and the polarographic analysis method. We found the most significant increase in ROS content in S. officinarum cells during temperature treatments (that did not cause immediate cell death in culture) was at 45 and 50 Β°C. The ROS content was largely determined by mitochondrial activity, as evidenced by a decrease in the electrochemical potential on the inner mitochondrial membrane (ΞΞ¨m), and a simultaneous decrease of ROS levels in cells under the carbonyl cyanide m-chlorophenyl hydrazine (CCCP) treatment. The decrease in the respiratory activity of cells under high temperatures was determined by the decrease of the cytochrome pathway (CP) contribution. It should be noted that the reduction in respiration rate at a temperature of 50 Β°C preceded the death of cells in the culture, and was not a consequence of itΠΡΡΠΎΠΊΠΈΠ΅ ΡΠ΅ΠΌΠΏΠ΅ΡΠ°ΡΡΡΡ ΡΠ²Π»ΡΡΡΡΡ Π²Π°ΠΆΠ½ΡΠΌΠΈ Π°Π±ΠΈΠΎΡΠΈΡΠ΅ΡΠΊΠΈΠΌΠΈ ΡΡΡΠ΅ΡΡΠΎΡΠ°ΠΌΠΈ, Π²Π»ΠΈΡΡΡΠΈΠΌΠΈ
Π½Π° ΡΠΎΡΡ, ΡΠ°Π·Π²ΠΈΡΠΈΠ΅ ΠΈ ΠΏΡΠΎΠ΄ΡΠΊΡΠΈΠ²Π½ΠΎΡΡΡ ΡΠ°ΡΡΠ΅Π½ΠΈΠΉ. Π£Π²Π΅Π»ΠΈΡΠ΅Π½ΠΈΠ΅ ΠΎΠ±ΡΠ°Π·ΠΎΠ²Π°Π½ΠΈΡ Π°ΠΊΡΠΈΠ²Π½ΡΡ
ΡΠΎΡΠΌ ΠΊΠΈΡΠ»ΠΎΡΠΎΠ΄Π°
(ΠΠ€Π) β ΠΎΠ΄Π½ΠΎ ΠΈΠ· ΠΏΠΎΡΠ»Π΅Π΄ΡΡΠ²ΠΈΠΉ ΠΈΡ
Π½Π΅Π³Π°ΡΠΈΠ²Π½ΠΎΠ³ΠΎ Π²Π»ΠΈΡΠ½ΠΈΡ. ΠΠ΄Π½Π°ΠΊΠΎ ΡΠΎ, ΠΊΠ°ΠΊΡΡ ΡΠΎΠ»Ρ ΡΡΠ³ΡΠ°ΡΡ ΠΠ€Π
Π² Π΄Π°Π»ΡΠ½Π΅ΠΉΡΠ΅ΠΉ ΡΡΠ΄ΡΠ±Π΅ ΠΊΠ»Π΅ΡΠΊΠΈ Π² ΡΡΠ»ΠΎΠ²ΠΈΡΡ
ΡΠ΅ΠΌΠΏΠ΅ΡΠ°ΡΡΡΠ½ΠΎΠ³ΠΎ ΡΡΡΠ΅ΡΡΠ°, Π·Π°Π²ΠΈΡΠΈΡ ΠΎΡ ΠΌΠ½ΠΎΠΆΠ΅ΡΡΠ²Π° Π²Π½ΡΡΡΠ΅Π½Π½ΠΈΡ
ΠΈ Π²Π½Π΅ΡΠ½ΠΈΡ
ΡΠ°ΠΊΡΠΎΡΠΎΠ². Π’Π°ΠΊΠΈΠΌ ΠΎΠ±ΡΠ°Π·ΠΎΠΌ, ΡΠ΅Π»ΡΡ Π΄Π°Π½Π½ΠΎΠΉ ΡΠ°Π±ΠΎΡΡ ΡΡΠ°Π»ΠΎ Π²ΡΡΠ²Π»Π΅Π½ΠΈΠ΅ Π²Π·Π°ΠΈΠΌΠΎΡΠ²ΡΠ·ΠΈ ΠΌΠ΅ΠΆΠ΄Ρ
ΡΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΠ΅ΠΌ ΠΠ€Π ΠΈ ΡΡΠ½ΠΊΡΠΈΠΎΠ½ΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ ΠΌΠΈΡΠΎΡ
ΠΎΠ½Π΄ΡΠΈΠΉ Π² ΠΊΠ»Π΅ΡΠΊΠ°Ρ
ΡΡΡΠΏΠ΅Π½Π·ΠΈΠΎΠ½Π½ΠΎΠΉ ΠΊΡΠ»ΡΡΡΡΡ
Saccharum officinarum ΠΏΡΠΈ Π΄Π΅ΠΉΡΡΠ²ΠΈΠΈ ΠΏΠΎΠ²ΡΡΠ΅Π½Π½ΡΡ
ΡΠ΅ΠΌΠΏΠ΅ΡΠ°ΡΡΡ. ΠΠ°Π½Π½ΠΎΠ΅ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠ΅ ΠΏΡΠΎΠ²ΠΎΠ΄ΠΈΠ»ΠΎΡΡ
Ρ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ ΡΠ»ΡΠΎΡΠ΅ΡΡΠ΅Π½ΡΠ½ΠΎΠΉ ΠΌΠΈΠΊΡΠΎΡΠΊΠΎΠΏΠΈΠΈ ΠΈ ΠΏΠΎΠ»ΡΡΠΎΠ³ΡΠ°ΡΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ Π°Π½Π°Π»ΠΈΠ·Π°. ΠΡΠ»ΠΎ ΠΎΠ±Π½Π°ΡΡΠΆΠ΅Π½ΠΎ,
ΡΡΠΎ ΡΠ΅ΠΌΠΏΠ΅ΡΠ°ΡΡΡΡ 45 ΠΈ 50 Β°C Π²ΡΠ·ΡΠ²Π°ΡΡ Π·Π½Π°ΡΠΈΡΠ΅Π»ΡΠ½ΠΎΠ΅ ΡΠ²Π΅Π»ΠΈΡΠ΅Π½ΠΈΠ΅ ΡΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΡ ΠΠ€Π Π² ΠΊΠ»Π΅ΡΠΊΠ°Ρ
S.
officinarum, ΡΡΠΎ, ΡΠ΅ΠΌ Π½Π΅ ΠΌΠ΅Π½Π΅Π΅, Π½Π΅ ΠΏΡΠΈΠ²ΠΎΠ΄ΠΈΡ ΠΊ Π½Π΅ΠΌΠ΅Π΄Π»Π΅Π½Π½ΠΎΠΉ Π³ΠΈΠ±Π΅Π»ΠΈ ΠΊΠ»Π΅ΡΠΎΠΊ Π² ΠΊΡΠ»ΡΡΡΡΠ΅. Π‘ΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΠ΅
ΠΠ€Π Π²ΠΎ ΠΌΠ½ΠΎΠ³ΠΎΠΌ ΠΎΠΏΡΠ΅Π΄Π΅Π»ΡΠ»ΠΎΡΡ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΡΡ ΠΌΠΈΡΠΎΡ
ΠΎΠ½Π΄ΡΠΈΠΉ, ΠΎ ΡΠ΅ΠΌ ΡΠ²ΠΈΠ΄Π΅ΡΠ΅Π»ΡΡΡΠ²ΡΠ΅Ρ ΡΠ½ΠΈΠΆΠ΅Π½ΠΈΠ΅
ΡΠ»Π΅ΠΊΡΡΠΎΡ
ΠΈΠΌΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΠΏΠΎΡΠ΅Π½ΡΠΈΠ°Π»Π° Π½Π° Π²Π½ΡΡΡΠ΅Π½Π½Π΅ΠΉ ΠΌΠΈΡΠΎΡ
ΠΎΠ½Π΄ΡΠΈΠ°Π»ΡΠ½ΠΎΠΉ ΠΌΠ΅ΠΌΠ±ΡΠ°Π½Π΅ (ΞΞ¨m) ΠΈ ΠΎΠ΄Π½ΠΎΠ²ΡΠ΅ΠΌΠ΅Π½Π½ΠΎΠ΅
ΡΠ½ΠΈΠΆΠ΅Π½ΠΈΠ΅ ΡΡΠΎΠ²Π½Ρ ΠΠ€Π Π² ΠΊΠ»Π΅ΡΠΊΠ°Ρ
ΠΏΡΠΈ ΠΎΠ±ΡΠ°Π±ΠΎΡΠΊΠ΅ ΠΊΠ°ΡΠ±ΠΎΠ½ΠΈΠ»ΡΠΈΠ°Π½ΠΈΠ΄-ΠΌ-
ΡΠ΅Π½ΠΈΠ»Π³ΠΈΠ΄ΡΠ°Π·ΠΎΠ½ΠΎΠΌ (Π‘Π‘Π‘Π ).
Π£ΠΌΠ΅Π½ΡΡΠ΅Π½ΠΈΠ΅ Π΄ΡΡ
Π°ΡΠ΅Π»ΡΠ½ΠΎΠΉ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ Π² ΠΊΠ»Π΅ΡΠΊΠ°Ρ
ΠΏΡΠΈ Π²ΡΡΠΎΠΊΠΎΡΠ΅ΠΌΠΏΠ΅ΡΠ°ΡΡΡΠ½ΠΎΠΌ Π²ΠΎΠ·Π΄Π΅ΠΉΡΡΠ²ΠΈΠΈ Π±ΡΠ»ΠΎ
ΠΎΠ±ΡΡΠ»ΠΎΠ²Π»Π΅Π½ΠΎ ΡΠ½ΠΈΠΆΠ΅Π½ΠΈΠ΅ΠΌ Π²ΠΊΠ»Π°Π΄Π° ΡΠΈΡΠΎΡ
ΡΠΎΠΌΠ½ΠΎΠ³ΠΎ ΠΏΡΡΠΈ (Π¦Π). Π‘Π»Π΅Π΄ΡΠ΅Ρ ΠΎΡΠΌΠ΅ΡΠΈΡΡ, ΡΡΠΎ ΡΠ½ΠΈΠΆΠ΅Π½ΠΈΠ΅
ΡΠΊΠΎΡΠΎΡΡΠΈ Π΄ΡΡ
Π°Π½ΠΈΡ ΠΏΡΠΈ ΡΠ΅ΠΌΠΏΠ΅ΡΠ°ΡΡΡΠ΅ 50 Β°C ΠΏΡΠ΅Π΄ΡΠ΅ΡΡΠ²ΠΎΠ²Π°Π»ΠΎ Π³ΠΈΠ±Π΅Π»ΠΈ ΠΊΠ»Π΅ΡΠΎΠΊ Π² ΠΊΡΠ»ΡΡΡΡΠ΅, Π° Π½Π΅ Π±ΡΠ»ΠΎ
Π΅Π΅ ΡΠ»Π΅Π΄ΡΡΠ²ΠΈΠ΅
Change of AOX1a Expression, Encoding Mitochondrial Alternative Oxidase, Influence on the Frost-Resistance of Arabidopsis Plants
The resistance of Arabidopsis thaliana (L.) Heynh (Columbia ecotype) plants: Col-0 line (wild type), AS-12 line (plants transformed with the construct carrying the AOX1a gene under control of the CAMV 35S promoter in the antisense orientation) and line XX-2 (plants transformed with the AOX1a gene construct in the sense orientation) (Umbach et al., 2005), to action of subzero temperature has been studied. It is shown that change of the AOX1a expression is accompanied by change of the AOX contribution in respiration and increase of the base frost-resistance of Arabidopsis plants. In the leaves of plants with overexpression of ΠΠΠ₯1Π° was reduced activity of total superoxide dismutase (SOD), but was increased activity of guaiacol peroxidase and was less content of hydrogen peroxide. It was found that cold hardening during 7 days at 5Β°C increases the resistance of plants to the subsequent action of subzero temperature regardless of ΠΠΠ₯1Π° expression degree. The hardening lead to activation of respiration, increase of the contribution of AOX in the respiration, a significant increase of the water-soluble carbohydrates content and increase of the activity SOD and total guaiacol peroxidases in leaves of all lines the plants. In hardened plants of Arabidopsis wild type and AOX1a transformants were detected differences in the contents of individual types of reactive oxygen species and the activity of antioxidant enzymes. The trend to decrease of hydrogen peroxide content in lines with altered expression of AOX1a was observed, but content of superoxide anion radical (SAR) was significantly lower in the AS-12 line compared with the Col-0 and XX-2 plants after hardening. The low content of SAR in leaves of AS-12 line was partly caused by increase of activity total SOD. Thus, we have identified differences in the basic frost-resistance of Arabidopsis plants with altered AOX1a expression, but significant differences in frost-resistance of hardened plants of wild-type and lines with altered AOX1a expression was not found. It was concluded that the frost-resistance of plants depends on the activity of AOX, but the decrease of its activity can be compensated by the activation of other protective systems including antioxidant enzymes