Perceptie van tussentaal in het gesproken Nederlands in Vlaanderen: een experimentele benadering van saillantie

In this paper, the results of a salience experiment in Flanders are reported. 80 informants were subjected to a qualitative interview in which they were asked to evaluate seven audio recordings, spoken in several regional versions of tussentaal (literally ‘in-between-language’) or in Standard Dutch. The informants had to judge which language variety was spoken in the recordings and they had to motivate on which features they based their judgment of the language used. This paper aims to show that research on salience has almost exclusively focused on whether a linguistic item is salient or not and that not enough attention has been paid to interpersonal variation in salience

De ideologische positie van Algemeen Nederlands in Vlaanderen: de standaardiseringsideologie onder druk?

The ideological position of Standard Dutch in Flanders: the standard language ideology under pressure? In this paper, the results of a perception study in Flanders are reported. 80 informants were subjected to a qualitative interview, to gain insight in the way Flemish language users judge on the situational appropriacy of several Dutch language varieties. The reported beliefs of the informants were compared to language ideological frameworks and they show an evolution in the standard language ideology (Milroy & Milroy, 1985), an ideology in which the standard language is considered to be the ideal and to be the only appropriate variety for formal and public situations. The informants still consider the standard language to be important and to be superior, but the variety according to them is not necessary for formal and public situations. Instead Tussentaal, lit. ‘in-between language’, has gained a position in the situational spectre

Standaardnederlands, de sleutel tot integratie in Vlaanderen? Over de uitdagingen van niet-standaardtaal voor NT2-leerders in Vlaanderen

Flanders strongly encourages newcomers to take language courses to learn the official language, on the assumption that certified competence in Dutch will enhance integration. However, in Dutch L2-education the focus lies on Standard Dutch, and this contrasts to everyday spoken encounters in Flanders, where Standard Dutch is rarely used. Despite the perceived importance of learning the official language of the host society, the attested contrast between policy and language reality has up to now not been given much attention. In this publication we go into the results of a linguistic-ethnographic study conducted with language learners living in East-Flanders. We show that non-standard Dutch used by L1-speakers of Dutch sometimes causes comprehension difficulties in their interactions with the language learners. Based on interactional and interview data, we reflect on the question if L2-education should pay more attention to non-standard Dutch in class

A generic polymer-protein ligation strategy for vaccine delivery

Although the field of cancer immunotherapy is intensively investigated, there is still a need for generic strategies that allow easy, mild and efficient formulation of vaccine antigens. Here we report on a generic polymer protein ligation strategy to formulate protein antigens into reversible polymeric conjugates for enhanced uptake by dendritic cells and presentation to CD8 T-cells. A N-hydroxypropylmethacrylamide (HPMA)-based copolymer was synthesized via RAFT polymerization followed by introduction of pyridyldisulfide moieties. To enhance ligation efficiency to ovalbumin, which is used as a model protein antigen, protected thiols were introduced onto lysine residues and deprotected in situ in the presence of the polymer. The ligation efficiency was compared for both the thiol-modified versus unmodified ovalbumin, and the reversibility was confirmed. Furthermore, the obtained nanoconjugates were tested in vitro for their interaction and association with dendritic cells, showing enhanced cellular uptake and antigen cross-presentation to CD8 T-cells

Evaluation of feline renal perfusion with contrast-enhanced ultrasonography and scintigraphy

Contrast-enhanced ultrasound (CEUS) is an emerging technique to evaluate tissue perfusion. Promising results have been obtained in the evaluation of renal perfusion in health and disease, both in human and veterinary medicine. Renal scintigraphy using Tc-99m-Mercaptoacetyltriglycine (MAG(3)) is another non-invasive technique that can be used to evaluate renal perfusion. However, no data are available on the ability of CEUS or Tc-99m-MAG(3) scintigraphy to detect small changes in renal perfusion in cats. Therefore, both techniques were applied in a normal feline population to evaluate detection possibilities of perfusion changes by angiotensin II (AT II). Contrast-enhanced ultrasound using a bolus injection of commercially available contrast agent and renal scintigraphy using Tc-99m-MAG(3) were performed in 11 healthy cats after infusion of 0,9% NaCl (control) and AT II. Angiotensin II induced changes were noticed on several CEUS parameters. Mean peak enhancement, wash-in perfusion index and wash-out rate for the entire kidney decreased significantly after AT II infusion. Moreover, a tendency towards a lower wash-in area-under-the curve was present. Renal scintigraphy could not detect perfusion changes induced by AT II. This study shows that CEUS is able to detect changes in feline renal perfusion induced by AT II infusion