Enhancing sustainable potato production—a case study in northern china

Potato is the fourth most important staple crop in China. To meet the increased demand and environmental objectives, potato production should be enhanced by sustainable practices that aim to maximize yield and resource use efficiencies and minimize environmental impacts. Most experiments so far have taken place on experimental stations, while on-farm experimentation is needed to evaluate, demonstrate and scale more sustainable practices. On-farm field experiments were conducted in two years (2017, 2018) in northern China to investigate and demonstrate the effects of different nitrogen (N) fertiliser and irrigation input levels on potato yield, quality, resource use efficiency and environmental impacts. The 2017 experimental results on one farmer’s field showed that under irrigated conditions, adding N fertiliser (from 0 to 267 kg ha−1) did not increase yield due to the high soil N supply, rather it reduced tuber quality. The 2018 experimental results, in which four additional farmers were involved, indicated that, under irrigated conditions, reducing N fertiliser from the current rates (189–252 kg ha−1) to lower levels (109–181 kg ha−1) did not affect yield nor quality; while further reducing N fertiliser inputs (to 9–117 kg ha−1) resulted in a yield reduction (18% on average) in some fields. In both years, irrigation improved tuber yield and quality compared to that under rainfed conditions. The nitrogen use efficiency was improved and N surplus was reduced by applying irrigation and reducing N fertiliser input. Farmers expressed they were willing to reduce N fertiliser input by 10–20%, and indicated that a widespread adaptation of drip irrigation is hindered by the high costs and labor requirements. Site-specific recommendations on optimum N fertiliser and irrigation management must be provided, which should preferably be based on regular quantitative monitoring of soil N supply and soil moisture content

Construction and Genetic Transformation of Potato Virus and Viroid Multi-resistant amiRNA Binary Expression Vectors

peer reviewedPotato viruses and viroid disease had seriously affected the production of potato in China. In order to cultivate virus and viroids antiviral new materials of potato. In this study, we utilized the strategy of artificial miRNA (amiRNA) with the silencing suppressor P25 protein and HC-pro protein which encoded by potato viral pathogens potato virus X (PVX) and potato virus Y (PVY) as the target, and we utilized 5'RACE technique to forecast the potential shear hot spots of P25 gene and HC-Pro gene. Then we used overlapping PCR technology, and also using Arabidopsis thaliana miR159a precursor as skeleton to design specific primers, so we could construct amiRNA expression vector which was respectively targeting P25 and HC-Pro genes. We constructed the amiRNA expression vector targeting the endogenous gene Virp1 of potato at the same time. And we could obtain amiRNAs three by seriesing three amiRNA expression vector which were construction completed. And then we transferred into the Agrobacterium strain EHA105 respectively, we used Agrobacterium mediated transformation of potato varieties Kexin13. Detected by PCR, the results showed that the target gene was successfully transferred into potato

Heterologous Expression of CLIBASIA_03915/CLIBASIA_04250 by Tobacco Mosaic Virus Resulted in Phloem Necrosis in the Senescent Leaves of Nicotiana benthamiana

Huanglongbing (HLB), also known as citrus greening, is the most notorious citrus disease worldwide. Candidatus Liberibacter asiaticus (CaLas) is a phloem-restricted bacterium associated with HLB. Because there is no mutant library available, the pathogenesis of CaLas is obscure. In this study, we employed tobacco mosaic virus (TMV) to express two mature secretion proteins CLIBASIA_03915 (m03915) and CLIBASIA_04250 (m04250) in Nicotiana benthamiana (N. benthamiana). Phloem necrosis was observed in the senescent leaves of N. benthamiana that expressed the two low molecular weight proteins, while no phloem necrosis was observed in the plants that expressed the control, green fluorescent protein (GFP). Additionally, no phloem necrosis was observed in the senescent leaves of N. benthamiana that expressed the null mutation of m03915 and frameshifting m04250. The subcellular localizations of m03915 and m04250 were determined by fusion with GFP using confocal microscopy. The subcellular localization of m03915 was found to be as free GFP without a nuclear localization sequence (NLS). However, m04250 did have an NLS. Yeast two-hybrid (Y2H) was carried out to probe the citrus proteins interacting with m03915 and m04250. Six citrus proteins were found to interact with m03915. The identified proteins were involved in the metabolism of compounds, transcription, response to abiotic stress, ubiquitin-mediated protein degradation, etc. The prey of m04250 was involved in the processing of specific pre-mRNAs. Identification of new virulence factors of CaLas will give insight into the pathogenesis of CaLas, and therefore, it will eventually help develop the HLB-resistant citrus