270 research outputs found

    Designing for Schadenfreude (or, how to express well-being and see if you're boring people)

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    This position paper presents two studies of content not normally expressed in status updatesā€”well-being and status feedbackā€”and considers how they may be processed, valued and used for potential quality-of-life benefits in terms of personal and social reflection and awareness. Do I Tweet Good? (poor grammar intentional) is a site investigating more nuanced forms of status feedback than current microblogging sites allow, towards understanding self-identity, reflection, and online perception. Healthii is a tool for sharing physical and emotional well-being via status updates, investigating concepts of self-reflection and social awareness. Together, these projects consider furthering the value of microblogging on two fronts: 1) refining the online personal/social networking experience, and 2) using the status update for enhancing the personal/social experience in the offline world, and considering how to leverage that online/offline split. We offer results from two different methods of study and target groupsā€”one co-workers in an academic setting, the other followers on Twitterā€”to consider how microblogging can become more than just a communication medium if it facilitates these types of reflective practice

    Presynaptic Localization of Sodium/Calcium Exchangers in Neuromuscular Preparations

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    Calcium ions play a critical role in neurotransmitter release. The cytosolic Ca 2+ concentration ([Ca2+]cyt) at nerve terminals must therefore be carefully controlled. Several different mechanisms, including a plasmalemmal Na/Ca exchanger, are involved in regulating [Ca2+]cyta We employed immunofluorescence microscopy with polyclonal antiserum raised against dog cardiac sarcolemmal Na/Ca exchanger to determine the distribution of the exchanger in vertebrate neuromuscular preparations. Our data indicate that the Na/Ca exchanger is concentrated at the neuromuscular junctions of the rat diaphragm. The exchanger is also present in the nonjunctional sarcolemma, but at a much lower concentration than in the junctional regions. Denervation markedly lowers the concentration of the exchanger in the junctional regions; this implies that the Na/Ca exchanger is concentrated in the presynaptic nerve terminals. In Xenopus laevis nerve and muscle cell cocultures, high concentrations of the exchanger are observed along the neurites as well as at the nerve terminals. The high concentrations of Na/Ca exchanger at presynaptic nerve terminals in vertebrate neuromuscular preparations suggest that the exchanger may participate in the Ca-dependent regulation of neurotransmitter release. The Na/Ca exchanger is also abundant in developing neurites and growth cones, where it may also be important for Ca2+ homeostasis
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