Bacteriophage and non-pathogenic Vibrio to control diseases in shrimp aquaculture

The study aimed to address the recurring outbreaks of microbial diseases in shrimp aquaculture in Bangladesh the study focused on the utilization of bacteriophages and non-pathogenic Vibrio. The bacteriophages were isolated from sewage water sample collected from shrimp farm, hatchery, and the JUST campus. The bacteriophages were tested for their ability to infect different Vibrio strains in order to assess their bacteriolytic activity. Non-pathogenic Vibrio strains were obtained from suspected diseased isolates collected from the south-western region of Bangladesh through PCR amplification. In laboratory tests, the bacteriophages successfully infected 91 % of the tested Vibrio strains (19 out of 21 strains). In the experimental unit, shrimp treated with phage prophylaxis and phage treatment demonstrated notable protection against AHPND and was able to survive a deadly bacterial challenge. A total of 35 suspected diseased isolates were tested, and PCR amplification revealed 6 non-pathogenic Vibrio strains. In field trials, cultured bacteriophages were applied at a concentration of 1.5×106 PFU/ml, while non-pathogenic Vibrio was applied at 5×105 CFU/ml. The trials showed increased protection against infections and no severe deaths during the adaptive research phase. The cultured shrimp were analyzed morphologically and showed a muscle gut ratio greater than 4:1. No abnormal deformities were observed in their appendages or overall body, suggesting their overall health and well-being. The bacteriological tests conducted on the shrimp samples (application of bacteriophages and non-pathogenic Vibrio) revealed that 18 % of them were infected with bacteria, primarily Vibrio cholerae, Vibrio parahaemolyticus, and other bacterial species. Despite this, the infections did not lead to a disease outbreak; PCR amplification showed negative results for AHPND, White Spot Syndrome Virus (WSSV), and Enterocytozoon hepatopenaei (EHP). The results highlight the potential of using bacteriophages and non-pathogenic Vibrio as a sustainable solution for preventing and controlling microbial diseases in shrimp aquaculture. Furthermore, this study will contribute valuable insights into the development of alternative strategies to combat antibiotic resistance and promote the growth of the shrimp industry in Bangladesh