23 research outputs found
Maintenance of respiratory control in mitochondria after rate zonal centrifugation
The respiratory control of rat liver mitochondria is lost when they are subjected to rate zonal centrifugation in a sucrose gradient (8.0% to 46.6%, w/w) at values for ω 2 t necessary for resolution. High sucrose concentration and high ω 2 t are both responsible. Respiratory control can be maintained in iso-osmotic Ficoll + 8.3% sucrose media, and after zonal centrifugation in such media at values of ω 2 t sufficient for resolution.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/44800/1/10863_2005_Article_BF01539061.pd