Molecular and morphological description of four new Ethmostigmus Pocock, 1898 (Chilopoda, Scolopendromorpha, Scolopendridae) species from China, with a key to Chinese species

Integrative of morphology and DNA data was using to define China Ethmostigmus centipedes. Two mitochondrial DNA loci (COI, 16S rRNA) and nuclear DNA locus (28S rRNA) were used to determine the phylogenetic position of any potential species using maximum likelihood and Bayesian inference approaches. Five species of China Ethmostigmus are new recognized: Ethmostigmus pygomenasoides Lewis, 1992 stat. nov., and four new species, viz. E. flavescens sp. nov., E. austroyunnanensis sp. nov., Ethmostigmus biocolor sp. nov. and E. motuoensis sp. nov. These species are described and keyed, and their distributions mapped

Molecular and morphological description of four new Ethmostigmus Pocock, 1898 (Chilopoda, Scolopendromorpha, Scolopendridae) species from China, with a key to Chinese species

Integrative of morphology and DNA data was using to define China Ethmostigmus centipedes. Two mitochondrial DNA loci (COI, 16S rRNA) and nuclear DNA locus (28S rRNA) were used to determine the phylogenetic position of any potential species using maximum likelihood and Bayesian inference approaches. Five species of China Ethmostigmus are new recognized: Ethmostigmus pygomenasoides Lewis, 1992 stat. nov., and four new species, viz. E. flavescens sp. nov., E. austroyunnanensis sp. nov., Ethmostigmus biocolor sp. nov. and E. motuoensis sp. nov. These species are described and keyed, and their distributions mapped

Antimicrobial peptide nanoparticles coated with macrophage cell membrane for targeted antimicrobial therapy of sepsis

Sepsis is a serious life-threatening disease. Bacterial infection is one of the main causes of sepsis. Although etiopathogenesis is gradually understood, the treatment is still scarce. Mortality caused by sepsis is still challenge. Herein, macrophage membrane coated antimicrobial peptide nanoparticles (M−AMPNP) was explored based on the specific binding of bacterial recognition receptors on macrophage membrane to bacteria. M−AMPNP was used to treat sepsis caused by bacterial infection. In vivo results showed that M−AMPNP was effectively delivered and retained at the site of infection by intravenous injection, which reduced the level of inflammatory factors and ultimately gave infected mice a significant survival advantage. M−AMPNP provides a potential strategy for targeted therapy of sepsis

Molecular detection of Xanthomonas oryzae pv. oryzae, Xanthomonas oryzae pv. oryzicola, and Burkholderia glumae in infected rice seeds and leaves

The polymerase chain reaction (PCR) is particularly useful for plant pathogen detection. In the present study, multiplex PCR and SYBR Green real-time PCR were developed to facilitate the simultaneous detection of three important rice pathogens, Xanthomonas oryzae pv. oryzae, X. oryzae pv. oryzicola, and Burkholderia glumae. The unique PCR primer sets were designed from portions of a putative glycosyltransferase gene of X. oryzae pv. oryzae, an AvrRxo gene of X. oryzae pv. oryzicola, and an internal transcribed spacer (ITS) sequence of B. glumae. Using a multiplex PCR assay, X. oryzae pv. oryzae, X. oryzae pv. oryzicola, and B. glumae were detected in one PCR reaction that contained the newly developed primer set mix. Using SYBR Green real-time PCR assays, X. oryzae pv. oryzae, X. oryzae pv. oryzicola, and B. glumae were detected at 1, 1, and 10 fg μL− 1, respectively. These newly designed molecular assays are sensitive and could be reliable tools for pathogen detection and disease forecasting

Study on the Preparation and Corrosion Resistance Properties of Superhydrophobic Coatings on Galvanized Steel

The method of atmospheric corrosion protection of metals has always been a research hot spot at home and abroad. In this paper, superhydrophobic coating is prepared on the surface of galvanized steel by chemical etching using 1H,1H,2H,2H-perfluorodecyltriethoxysilane (PFDTES), graphene oxide (GO), anhydrous ethanol and water-based varnish as the main raw materials. The constitution of the superhydrophobic coating surface and the corrosion resistance of the steel are studied by electrochemical testing, X-ray diffraction (XRD), scanning electron microscopy (SEM), X-ray energy dispersive spectroscopy (EDS) and atomic force microscopy (AFM). Meanwhile, COMSOL software is used for the simulation of experiment. The results show that the surface of the superhydrophobic coating is composed of micro–nano sized papillary bulges, which play an important role in the improvement of metal corrosion resistance. The superhydrophobic coating effectively improves the alternating current impedance of the electrode and reduces the corrosion rate of the substrate. In addition, the results calculated by COMSOL software consist of the experimental results

Idarubicin and IR780 co-loaded PEG-b-PTMC nanoparticle for non-Hodgkin’s lymphoma therapy by photothermal/photodynamic strategy

A certain proportion of non-Hodgkin’s lymphoma (NHL) patients will relapse after standard chemotherapy with an anthracycline-based regimen. Therefore, identifying novel therapeutic methods are a vital topic for improving therapeutic effects of NHL. IR780, a near-infrared dye, can be used as a photosensitizer both for photothermal therapy (PTT) and photodynamic therapy (PDT). In this study, we designed an effective nanoparticle system for carrying IR780 and Idarubicin, thus hoping to combine PTT/PDT and chemotherapy to treat NHL. This nanosystem consisted of PEG-b-PTMC nanoparticles. IR780 and Idarubicin (IDA) were loaded separately into PEG-b-PTMC nanoparticles, which had regular sphere shapes and relatively small sizes. Upon near-infrared laser irradiation at 808 nm, PEG-b-PTMC nanoparticle (IR780 + IDA NPs) showed strong PTT/PDT efficacy with high-efficiency endocytosis and increasing reactive oxygen species (ROS) generation. By the evaluation of the photothermal conversion, we can confirm that IR780 + IDA NPs has been successfully delivered and exhibited significant synergistic effects on inhibiting cell proliferation in Raji and U937 cells. In vivo experiments have also demonstrated that IR780 + IDA NPs has a significant inhibitory effect on lymphoma and has a good biological safety. In summary, we put forward a therapeutic strategy for NHL treatment by combining PTT/PDT and chemotherapy