Sustainable Development of Mountain Hotels through the Implementation of International Management Standards: The Romanian Case

This paper aims to identify the current level of implementation of the principles of sustainable development among hotels in the mountain area of Romania, how these standards influence the occupancy rate with the help of communication strategies, and to what extent the implementation of international management standards leads to an increase in the profitability rate. The research was carried out on the basis of questionnaires addressed to the hotels in the mountain areas of Romania, with information being collected from a number of 77 hotels, rated between 2 and 5 stars. The results obtained from the research highlighted the existence of positive correlations, both between the use of a communication strategy on the sustainable development objectives and the occupancy rate, as well as between the implementation of international management standards and the increase of the profitability rate

α-Chitosan and β-Oligochitosan Mixtures-Based Formula for In Vitro Assessment of Melanocyte Cells Response

Chitosan is a natural polymer with numerous biomedical applications. The cellular activity of chitosan has been studied in various types of cancer, including melanoma, and indicates that these molecules can open new perspectives on antiproliferative action and anticancer therapy. This study analyzes how different chitosan conformations, such as α-chitosan (CH) or β-oligochitosan (CO), with various degrees of deacetylation (DDA) and molar mass (MM), both in different concentrations and in CH–CO mixtures, influence the cellular processes of SK-MEL-28 melanocytes, to estimate the reactivity of these cells to the applied treatments. The in vitro evaluation was carried out, aiming at the cellular metabolism (MTT assay), cellular morphology, and chitinase-like glycoprotein YKL-40 expression. The in vitro effect of the CH–CO mixture application on melanocytes is obvious at low concentrations of α-chitosan/β-oligochitosan (1:2 ratio), with the cell’s response supporting the hypothesis that β-oligo-chitosan amplifies the effect. This oligochitosan mixture, favored by the β conformation and its small size, penetrates faster into the cells, being more reactive when interacting with some cellular components. Morphological effects expressed by the loss of cell adhesion and the depletion of YKL-40 synthesis are significant responses of melanocytes. β-oligochitosan (1.5 kDa) induces an extension of cytophysiological effects and limits the cell viability compared to α-chitosan (400–900 kDa). Statistical analysis using multivariate techniques showed differences between the CH samples and CH–CO mixtures