Global Innovation Policy Index

Ranks fifty-five nations' strategies to boost innovation capacity: policies on trade, scientific research, information and communications technologies, tax, intellectual property, domestic competition, government procurement, and high-skill immigration

Diamagnetic repulsion of particles for multilaminar flow assays

© The Royal Society of Chemistry. We demonstrate diamagnetic repulsion forces for performing continuous multilaminar flow assays on particles based on their intrinsic properties and with a simple setup. The platform could be applied to sandwich assays on polystyrene particles, and to cell-based assays via their suspension in biologically benign magnetic media

Marine Benthic Habitat Mapping of Muir Inlet, Glacier Bay National Park and Preserve, Alaska With an Evaluation of the Coastal and Marine Ecological Classification Standard III

Seafloor geology and potential benthic habitats were mapped in Muir Inlet, Glacier Bay National Park and Preserve, Alaska, using multibeam sonar, ground-truth information, and geological interpretations. Muir Inlet is a recently deglaciated fjord that is under the influence of glacial and paraglacial marine processes. High glacially derived sediment and meltwater fluxes, slope instabilities, and variable bathymetry result in a highly dynamic estuarine environment and benthic ecosystem. We characterize the fjord seafloor and potential benthic habitats using the Coastal and Marine Ecological Classification Standard (CMECS) recently developed by the National Oceanic and Atmospheric Administration (NOAA) and NatureServe. Substrates within Muir Inlet are dominated by mud, derived from the high glacial debris flux. Water-column characteristics are derived from a combination of conductivity temperature depth (CTD) measurements and circulation-model results. We also present modern glaciomarine sediment accumulation data from quantitative differential bathymetry. These data show Muir Inlet is divided into two contrasting environments: a dynamic upper fjord and a relatively static lower fjord. The accompanying maps represent the first publicly available high-resolution bathymetric surveys of Muir Inlet. The results of these analyses serve as a test of the CMECS and as a baseline for continued mapping and correlations among seafloor substrate, benthic habitats, and glaciomarine processes

Comprehension as social and intellectual practice: Rebuilding curriculum in low socioeconomic and cultural minority schools

This article reframes the concept of comprehension as a social and intellectual practice. It reviews current approaches to reading instruction for linguistically and culturally diverse and low socioeconomic students, noting an emphasis on comprehension as autonomous skills. The Four Resources model (Freebody & Luke, 1990) is used to make the case for the integration of comprehension instruction with an emphasis on student cultural and community knowledge, and substantive intellectual and sociocultural content in elementary school curricula. Illustrations are drawn from research underway on the teaching of literacy in primary schools in low SES communities

A Broad Spectrum Racemase in \u3cem\u3ePseudomonas putida\u3c/em\u3e KT2440 Plays a Key Role in Amino Acid Catabolism

The broad-spectrum amino acid racemase (Alr) of Pseudomonas putida KT2440 preferentially interconverts the L- and D-stereoisomers of Lys and Arg. Despite conservation of broad-spectrum racemases among bacteria, little is known regarding their physiological role. Here we explore potential functional roles for Alr in P. putida KT2440. We demonstrate through cellular fractionation that Alr enzymatic activity is found in the periplasm, consistent with its putative periplasm targeting sequence. Specific activity of Alr is highest during exponential growth, and this activity corresponds with an increased accumulation of D-Lys in the growth medium. An alr gene knockout strain (Δalr) was generated and used to assess potential roles for the alr gene in peptidoglycan structure, producing soluble signaling compounds, and amino acid metabolism. The stationary phase peptidoglycan structure did not differ between wild-type and Δalr strains, indicating that products resulting from Alr activity are not incorporated into peptidoglycan under these conditions. RNA-seq was used to assess differences in the transcriptome between the wild-type and Δalr strains. Genes undergoing differential expression were limited to those involved in amino acid metabolism. The Δalr strain exhibited a limited capacity for catabolism of L-Lys and L-Arg as the sole source of carbon and nitrogen. This is consistent with a predicted role for Alr in catabolism of L-Lys by virtue of its ability to convert L-Lys to D-Lys, which is further catabolized through the L-pipecolate pathway. The metabolic profiles here also implicate Alr in catabolism of L-Arg, although the pathway by which D-Arg is further catabolized is not clear at this time. Overall, data presented here describe the primary role of Alr as important for basic amino acid metabolism

Amino Acid Racemase Enzyme Assays

Amino acid racemases are enzymes that invert the α-carbon stereochemistry of amino acids (AAs), interconverting amino acids between their L- and D-enantiomers in a reversible reaction. In bacteria, they are known to have catabolic physiological functions but are also involved in the synthesis of many D-AAs, including D-glutamate and D-alanine, which are necessary components of the peptidoglycan layer of the bacterial cell wall. As such, amino acid racemases represent significant targets for the development of bactericidal compounds. Amino acid racemases are also regarded by the biotechnological industry as important catalysts for the production of economically relevant D-AAs. Here, we provide a detailed protocol using high performance liquid chromatography (HPLC) and 1-fluoro-2,4-dinitrophenyl-5-L-alanine amide (FDAA, also Marfey’s reagent) for the characterization of novel amino acid racemases. The protocol described here was designed to obtain accurate kinetic parameters (kcat, KM values). Enzyme concentrations and reaction times were optimized so as to minimize the reverse reaction, which can confound results when measuring racemase reactions

Tank Systems on Shrimp Farms Are Effective for Extension Demonstrations in Aquaculture

Numerous approaches can be implemented to carry out on-farm Extension demonstrations in aquaculture. The design and approach used are often governed by the problem or question that needs to be addressed as well as budgetary constraints within Extension programs. West Alabama is home to a unique inland marine shrimp industry that uses a low-salinity artesian groundwater source to raise shrimp. Demonstration and technology transfer have been carried out on commercial shrimp farms for nearly 20 years using low-budget on-levee tank systems operated by Extension personnel using this unique water source. On-farm tank demonstrations can be an effective tool for Extension professionals working in aquaculture, especially where unique circumstances exist