First order convergence of matroids

The model theory based notion of the first order convergence unifies the notions of the left-convergence for dense structures and the Benjamini-Schramm convergence for sparse structures. It is known that every first order convergent sequence of graphs with bounded tree-depth can be represented by an analytic limit object called a limit modeling. We establish the matroid counterpart of this result: every first order convergent sequence of matroids with bounded branch-depth representable over a fixed finite field has a limit modeling, i.e., there exists an infinite matroid with the elements forming a probability space that has asymptotically the same first order properties. We show that neither of the bounded branch-depth assumption nor the representability assumption can be removed.Comment: Accepted to the European Journal of Combinatoric

Plant cathepsin B, a versatile protease

Plant proteases are essential enzymes that play key roles during crucial phases of plant life. Some proteases are mainly involved in general protein turnover and recycle amino acids for protein synthesis. Other proteases are involved in cell signalling, cleave specific substrates and are key players during important genetically controlled molecular processes. Cathepsin B is a cysteine protease that can do both because of its exopeptidase and endopeptidase activities. Animal cathepsin B has been investigated for many years, and much is known about its mode of action and substrate preferences, but much remains to be discovered about this potent protease in plants. Cathepsin B is involved in plant development, germination, senescence, microspore embryogenesis, pathogen defence and responses to abiotic stress, including programmed cell death. This review discusses the structural features, the activity of the enzyme and the differences between the plant and animal forms. We discuss its maturation and subcellular localisation and provide a detailed overview of the involvement of cathepsin B in important plant life processes. A greater understanding of the cell signalling processes involving cathepsin B is needed for applied discoveries in plant biotechnology

Drosophila melanogaster cellular repressor of E1A-stimulated genes is a lysosomal protein essential for fly development

AbstractMammalian cellular repressor of E1A-stimulated genes is a lysosomal glycoprotein implicated in cellular growth and differentiation. The genome of the fruit fly Drosophila melanogaster encodes a putative orthologue (dCREG), suggesting evolutionarily conserved physiological functions of this protein. In D. melanogaster S2 cells, dCREG was found to localize in lysosomes. Further studies revealed that intracellular dCREG is subject of proteolytic maturation. Processing and turnover could be substantially reduced by RNAi-mediated silencing of cathepsin L. In contrast to mammalian cells, lysosomal delivery of dCREG does not depend on its carbohydrate moiety. Furthermore, depletion of the putative D. melanogaster lysosomal sorting receptor lysosomal enzyme receptor protein did not compromise cellular retention of dCREG. We also investigated the developmental consequences of dCREG ablation in whole D. melanogaster flies. Ubiquitous depletion of dCREG proved lethal at the late pupal stage once a knock-down efficiency of >95% was achieved. These results demonstrate that dCREG is essential for proper completion of fly development

Drosophila melanogaster cellular repressor of E1A-stimulated genes is a lysosomal protein essential for fly development

AbstractMammalian cellular repressor of E1A-stimulated genes is a lysosomal glycoprotein implicated in cellular growth and differentiation. The genome of the fruit fly Drosophila melanogaster encodes a putative orthologue (dCREG), suggesting evolutionarily conserved physiological functions of this protein. In D. melanogaster S2 cells, dCREG was found to localize in lysosomes. Further studies revealed that intracellular dCREG is subject of proteolytic maturation. Processing and turnover could be substantially reduced by RNAi-mediated silencing of cathepsin L. In contrast to mammalian cells, lysosomal delivery of dCREG does not depend on its carbohydrate moiety. Furthermore, depletion of the putative D. melanogaster lysosomal sorting receptor lysosomal enzyme receptor protein did not compromise cellular retention of dCREG. We also investigated the developmental consequences of dCREG ablation in whole D. melanogaster flies. Ubiquitous depletion of dCREG proved lethal at the late pupal stage once a knock-down efficiency of >95% was achieved. These results demonstrate that dCREG is essential for proper completion of fly development

Superior SARS-CoV-2 RBD antigen designs for highly specific, quantitative serotests

Quantitative high-quality SARS-CoV-2 serotests that are easy-to-implement have been gaining great importance as means to characterize and monitor the magnitude of infection- or vaccine-induced immunity over time and are of particular interest for academic laboratories doing COVID-19 research or small diagnostic laboratories with basic equipment Please click Download on the upper right corner to see the full abstract