MUSICOTERAPIA: DA PRESERVAÇÃO À RECUPERAÇÃO DA SAÚDE

A musicoterapia e a utilizacao da musica em um contexto clinico, educacional e social para prevencao e apoio a problemas de saude mental, promovendo qualidade de vida e bem estar. O emprego de sons e melodias tem o intuito de aliviar algum sintoma e tratar doenças, podendo ser empregada em adultos, crianças e idosos. Portanto, o objetivo deste projeto é proporcionar, aos internos da Santa Casa de Misericordia e do Asilo Sao Vicente de Paulo de Jacarezinho/Paraná, entretenimento, distração, socialização e melhora do bem- estar, prevenindo e aliviando sintomas relacionados as doencas, contribuindo para uma melhor qualidade de vida. São realizadas sessões de duas horas de musicoterapia com instrumento musical e canto, uma vez por semana, em cada local, além de reuniões semanais para preparação das sessões. Acredita-se que o projeto de musicoterapia tem alcançado os seus objetivos, principalmente no que tange a melhora do cognitivo e qualidade de vida dos internos e desenvolvimento de um espírito humanista nos universitários. Esse fato pode ser observado pelos depoimentos de responsáveis pelas instituições, internos e acompanhantes, além da observação da efetiva participa

Mecanismos Disciplinadores da Gestão de Custos Interorganizacionais e Economia dos Custos de Transação: um ensaio teórico

This article aimed to verify whether the disciplining mechanisms present in the conceptual structure of the Interorganizational Cost Management (ICM) are related to the governance mechanisms established through the Transaction Cost Economics (TCE), as tools to safeguard contractual relationships. The research was developed through a theoretical essay, based on bibliographic research. Its main contribution to the literature is the identification of relationships between consolidated TCE mechanisms, which aims to verify the way the transactions occur and the recent ICM mechanisms, aimed at optimizing the return of the value chain beyond the limits of the company through cost management. In conclusion, it was verified that, despite the recent literature on ICM, the disciplining mechanisms used by this Strategic Cost Management artifact are in line with the governance mechanisms used by the TCE, in relation and synergy with the different mechanisms analyzed.El presente artículo buscó verificar si los mecanismos disciplinarios, presentes en la estructura conceptual de la Gestión de Costos Interorganizacionales (GCI), están relacionados a los mecanismos de gobernanza previstos por la Economía de los Costos de Transacción (ECT), en la condición de instrumentos para salvaguardar relaciones contractuales. El trabajo fue desarrollado por medio de un ensayo teórico, con base en investigación bibliográfica, teniendo como contribución principal para la literatura, la identificación de la existencia de relacionamientos entre mecanismos ya consolidados de la ECT, la cual busca verificar la forma como ocurren las transacciones, y los mecanismos recientes de la GCI, que busca, por medio de la gestión de costos, la optimización del retorno total de la cadena de valor más allá de los límites de la propia empresa. Como conclusión del trabajo, se constató que, aun siendo reciente la literatura acerca de la GCI, los mecanismos disciplinarios utilizados por este artefacto de la Gestión Estratégica de Costos convergen hacia los mecanismos de gobernanza utilizados por la ECT, al presentar relación y sinergia en diferentes mecanismos analizados.O presente artigo buscou verificar se os mecanismos disciplinadores presentes na estrutura conceitual da Gestão de Custos Interorganizacionais (GCI) estão relacionados aos mecanismos de governança previstos pela Economia dos Custos de Transação (ECT), enquanto instrumentos para salvaguardar relações contratuais. O trabalho foi desenvolvido por meio de um ensaio teórico, com base em pesquisa bibliográfica, tendo como contribuição principal para a literatura, a identificação da existência de relacionamentos entre mecanismos já consolidados da ECT, a qual busca verificar a forma como ocorrem as transações, e os mecanismos recentes da GCI, que busca por meio do gerenciamento de custos, a otimização do retorno total da cadeia de valor além dos limites da própria empresa. Como conclusão do trabalho constatou-se que, mesmo sendo recente a literatura acerca da GCI, os mecanismos disciplinadores utilizados por este artefato da Gestão Estratégica de Custos vão ao encontro dos mecanismos de governança utilizados pela ECT, ao apresentarem relação e sinergia em diversos dos mecanismos analisados

Origins of the Xylella fastidiosa Prophage-Like Regions and Their Impact in Genome Differentiation

Xylella fastidiosa is a Gram negative plant pathogen causing many economically important diseases, and analyses of completely sequenced X. fastidiosa genome strains allowed the identification of many prophage-like elements and possibly phage remnants, accounting for up to 15% of the genome composition. To better evaluate the recent evolution of the X. fastidiosa chromosome backbone among distinct pathovars, the number and location of prophage-like regions on two finished genomes (9a5c and Temecula1), and in two candidate molecules (Ann1 and Dixon) were assessed. Based on comparative best bidirectional hit analyses, the majority (51%) of the predicted genes in the X. fastidiosa prophage-like regions are related to structural phage genes belonging to the Siphoviridae family. Electron micrograph reveals the existence of putative viral particles with similar morphology to lambda phages in the bacterial cell in planta. Moreover, analysis of microarray data indicates that 9a5c strain cultivated under stress conditions presents enhanced expression of phage anti-repressor genes, suggesting switches from lysogenic to lytic cycle of phages under stress-induced situations. Furthermore, virulence-associated proteins and toxins are found within these prophage-like elements, thus suggesting an important role in host adaptation. Finally, clustering analyses of phage integrase genes based on multiple alignment patterns reveal they group in five lineages, all possessing a tyrosine recombinase catalytic domain, and phylogenetically close to other integrases found in phages that are genetic mosaics and able to perform generalized and specialized transduction. Integration sites and tRNA association is also evidenced. In summary, we present comparative and experimental evidence supporting the association and contribution of phage activity on the differentiation of Xylella genomes

The Genome of Anopheles darlingi, the main neotropical Malaria vector

Anopheles darlingi is the principal neotropical malaria vector, responsible for more than a million cases of malaria per year on the American continent. Anopheles darlingi diverged from the African and Asian malaria vectors ∼100 million years ago (mya) and successfully adapted to the New World environment. Here we present an annotated reference A. darlingi genome, sequenced from a wild population of males and females collected in the Brazilian Amazon. A total of 10 481 predicted protein-coding genes were annotated, 72% of which have their closest counterpart in Anopheles gambiae and 21% have highest similarity with other mosquito species. In spite of a long period of divergent evolution, conserved gene synteny was observed between A. darlingi and A. gambiae. More than 10 million single nucleotide polymorphisms and short indels with potential use as genetic markers were identified. Transposable elements correspond to 2.3% of the A. darlingi genome. Genes associated with hematophagy, immunity and insecticide resistance, directly involved in vector–human and vector–parasite interactions, were identified and discussed. This study represents the first effort to sequence the genome of a neotropical malaria vector, and opens a new window through which we can contemplate the evolutionary history of anopheline mosquitoes. It also provides valuable information that may lead to novel strategies to reduce malaria transmission on the South American continent. The A. darlingi genome is accessible a

Balanço energético e pegada de carbono nos sistemas de produção integrada e convencional de feijão-comum irrigado

The objective of this work was to evaluate the contribution of an integrated farming system (PI) of common bean, in comparison with convectional farming, as to energy use, energy efficiency, and carbon footprint, considered from input manufacturing to grain harvest in the farms, excluding transport. Four farmswere selected in Cristalina, state of Goiás, Brazil, during the third harvest season of common bean, in 2009. The PI system reduced the average energy use by 3.1%, in comparison with the conventional system. Values ranged from 22,759.9 to 25,518.4 Mg ha-1. Mineral N fertilization with 6,584.0 MJ ha-1, agricultural operations with 5,309.4 MJ ha-1, and irrigation with 4,961.4 MJ ha-1 had the largest participation in energy use. Energy efficiency in PI (2.16) was higher than in the conventional system (2.01). There was no significant difference between the two systems as to carbon footprint per hectare, but carbon footprint per kilogram of yielded bean in PI (0.301 kg CO2-eq kg-1) was significantly lower than in conventional system (0.325 kg CO2-eq kg-1). The improvement of N fertilizer use efficiency by N biological fixation and the rationalization of machinery are the production system components that contributes the most for the reduction of carbon footprint.O objetivo deste trabalho foi avaliar a contribuição do sistema de produção integrada de feijão‑comum (PI), em comparação ao sistema de produção convencional, quanto ao uso de energia, à eficiência energética e à pegada de carbono, contabilizados desde a fabricação de insumos até a colheita de grãos nas fazendas, tendo-se excluído o transporte. Foram selecionadas quatro fazendas em Cristalina, GO, na terceira época de produção de feijão, em 2009. O sistema PI reduziu o uso médio de energia em 3,1%, em comparação ao convencional. Os resultados variaram de 22.759,9 a 25.518,4 MJ ha-1. A adubação nitrogenada com 6.584,0 MJ ha-1, as operações mecanizadas com 5.309,4 MJ ha-1 e a irrigação com 4.961,4 MJ ha-1 tiveram a maior participação quanto ao uso de energia. A eficiência energética em PI (2,16) foi superior à observada no sistema convencional (2,01). Não houve diferença significativa entre os dois sistemas quanto à pegada de carbono por hectare, mas a pegada de carbono por quilograma de feijão produzido no PI (0,301 kg CO2-eq kg-1) foi significativamente menor do que no convencional (0,325 kg CO2-eq kg-1). A melhora da eficiência da adubação nitrogenada, por meio do uso de fixação biológica de N, e a racionalização da mecanização são os componentes dos sistemas de produção que mais contribuem para a diminuição da pegada de carbono

De novo transcriptome analysis of Hevea brasiliensis tissues by RNA-seq and screening for molecular markers

Abstract\ud \ud Background\ud The rubber tree, Hevea brasiliensis, is a species native to the Brazilian Amazon region and it supplies almost all the world’s natural rubber, a strategic raw material for a variety of products. One of the major challenges for developing rubber tree plantations is adapting the plant to biotic and abiotic stress. Transcriptome analysis is one of the main approaches for identifying the complete set of active genes in a cell or tissue for a specific developmental stage or physiological condition.\ud \ud \ud Results\ud Here, we report on the sequencing, assembling, annotation and screening for molecular markers from a pool of H. brasiliensis tissues. A total of 17,166 contigs were successfully annotated. Then, 2,191 Single Nucleotide Variation (SNV) and 1.397 Simple Sequence Repeat (SSR) loci were discriminated from the sequences. From 306 putative, mainly non-synonymous SNVs located in CDS sequences, 191 were checked for their ability to characterize 23 Hevea genotypes by an allele-specific amplification technology. For 172 (90%), the nucleotide variation at the predicted genomic location was confirmed, thus validating the different steps from sequencing to the in silico detection of the SNVs.\ud \ud \ud Conclusions\ud This is the first study of the H. brasiliensis transcriptome, covering a wide range of tissues and organs, leading to the production of the first developed SNP markers. This process could be amplified to a larger set of in silico detected SNVs in expressed genes in order to increase the marker density in available and future genetic maps. The results obtained in this study will contribute to the H. brasiliensis genetic breeding program focused on improving of disease resistance and latex yield.CNPqPROSULCAPESFUNDHER

Predicting the Proteins of Angomonas deanei, Strigomonas culicis and Their Respective Endosymbionts Reveals New Aspects of the Trypanosomatidae Family

Endosymbiont-bearing trypanosomatids have been considered excellent models for the study of cell evolution because the host protozoan co-evolves with an intracellular bacterium in a mutualistic relationship. Such protozoa inhabit a single invertebrate host during their entire life cycle and exhibit special characteristics that group them in a particular phylogenetic cluster of the Trypanosomatidae family, thus classified as monoxenics. in an effort to better understand such symbiotic association, we used DNA pyrosequencing and a reference-guided assembly to generate reads that predicted 16,960 and 12,162 open reading frames (ORFs) in two symbiont-bearing trypanosomatids, Angomonas deanei (previously named as Crithidia deanei) and Strigomonas culicis (first known as Blastocrithidia culicis), respectively. Identification of each ORF was based primarily on TriTrypDB using tblastn, and each ORF was confirmed by employing getorf from EMBOSS and Newbler 2.6 when necessary. the monoxenic organisms revealed conserved housekeeping functions when compared to other trypanosomatids, especially compared with Leishmania major. However, major differences were found in ORFs corresponding to the cytoskeleton, the kinetoplast, and the paraflagellar structure. the monoxenic organisms also contain a large number of genes for cytosolic calpain-like and surface gp63 metalloproteases and a reduced number of compartmentalized cysteine proteases in comparison to other TriTryp organisms, reflecting adaptations to the presence of the symbiont. the assembled bacterial endosymbiont sequences exhibit a high A+T content with a total of 787 and 769 ORFs for the Angomonas deanei and Strigomonas culicis endosymbionts, respectively, and indicate that these organisms hold a common ancestor related to the Alcaligenaceae family. Importantly, both symbionts contain enzymes that complement essential host cell biosynthetic pathways, such as those for amino acid, lipid and purine/pyrimidine metabolism. These findings increase our understanding of the intricate symbiotic relationship between the bacterium and the trypanosomatid host and provide clues to better understand eukaryotic cell evolution.Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)ERC AdG SISYPHEUniv Fed Rio de Janeiro, Inst Biofis Carlos Chagas Filho, Lab Ultraestrutura Celular Hertha Meyer, BR-21941 Rio de Janeiro, BrazilUniv Fed Rio de Janeiro, Inst Biofis Carlos Chagas Filho, Lab Metab Macromol Firmino Torres de Castro, BR-21941 Rio de Janeiro, BrazilLab Bioinformat, Lab Nacl Computacao Cient, Rio de Janeiro, BrazilINRIA Grenoble Rhone Alpes, BAMBOO Team, Villeurbanne, FranceUniv Lyon 1, CNRS, UMR5558, Lab Biometrie & Biol Evolut, F-69622 Villeurbanne, FranceUniv Estadual Campinas, Inst Biol, Dept Genet Evolucao & Bioagentes, São Paulo, BrazilUniv São Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Ciencias Farmaceut, São Paulo, BrazilLab Nacl Ciencia & Tecnol Bioetano, São Paulo, BrazilUniv Fed Minas Gerais, Inst Ciencias Biol, Dept Bioquim & Imunol, Belo Horizonte, MG, BrazilUniv Fed Goias, Inst Ciencias Biol, Mol Biol Lab, Goiania, Go, BrazilFundacao Oswaldo Cruz, Inst Carlos Chagas, Lab Biol Mol Tripanossomatideos, Curitiba, Parana, BrazilFundacao Oswaldo Cruz, Inst Carlos Chagas, Lab Genom Func, Curitiba, Parana, BrazilUniv Estadual Campinas, Ctr Pluridisciplinar Pesquisas Quim Biol & Agr, São Paulo, BrazilUniv Fed Minas Gerais, Inst Ciencias Biol, Dept Parasitol, Belo Horizonte, MG, BrazilUniv Fed Santa Catarina, Dept Microbiol Imunol & Parasitol, Ctr Ciencias Biol, Lab Protozool & Bioinformat, Florianopolis, SC, BrazilUniv Fed Vicosa, Dept Bioquim & Biol Mol, Ctr Ciencias Biol & Saude, Vicosa, MG, BrazilInst Butantan, Lab Especial Ciclo Celular, São Paulo, BrazilUniv São Paulo, Dept Biol, Fac Filosofia Ciencias & Letras Ribeirao Preto, São Paulo, BrazilUniversidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol Imunol & Parasitol, São Paulo, BrazilUniversidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol Imunol & Parasitol, São Paulo, BrazilWeb of Scienc