13 research outputs found
Enhancement of Yield, Phytochemical Content and Biological Activity of a Leafy Vegetable (Beta vulgaris L. var. cycla) by Using Organic Amendments as an Alternative to Chemical Fertilizer
This study evaluates the effect of a chemical fertilizer (ammonium nitrate), a compost (vermicompost from cattle manure) and two biochars (from vine prunings and wood chips, respectively), applied to the soil alone or in mixture, on the yield, phytochemical content and biological activity of Beta vulgaris L. var. cycla (Swiss chard). The respective treatments, each replicated four times, were arranged according to a completely randomized block design. Results showed that vermicompost, both alone and in mixture with vine pruning biochar, significantly increased yield parameters (plant height and leaf area) and yield over the untreated soil and the biochars alone, similar to ammonium nitrate. Moreover, vermicompost, both alone and in mixture, respectively, with the two biochars, determined lower total N and NO3â contents than ammonium nitrate, both alone and in mixture, respectively, with the two biochars. In particular, NO3â content was within the safe thresholds fixed for leafy vegetables by the European Commission to prevent any adverse implication on human health from dietary NO3â exposure. The biochars alone resulted in very low yield and leaf total N content, likely due to a limited release of N for plant uptake, also evidenced by the undetectable NO3â leaf content, similarly shown by plants grown in untreated soil. Vermicompost, alone or in mixture, respectively, with the two biochars, increased the content of specialized metabolites, with a positive effect on antioxidant activity. The organic amendments, particularly compost, could be an alternative to chemical fertilizers to reach a trade-off between yield, nutritional and health qualities in Swiss chard, meeting the needs of farmers and consumers as well as the targets for sustainable food production
Productivity and quality of different tomato cultivars under intercropping system with maize and dry farming conditions in Southern Italy
This research, carried out in a typical Mediterranean environment of Southern Italy, reports some quantitative and qualitative traits of three local tomato cultivars (âArsicoloâ, âCrovareseâ, and âSan Marzanoâ) plus a commercial one (âDatterinoâ) cultivated without irrigation and intercropped with maize. For each cultivar, in addition to the determination of fruit production, tomato paste and âconservaâ paste were prepared using a traditional technique of the Tanagro Valley (province of Salerno, Southern Italy). Results highlighted that âArsicoloâ showed the best fruit yield (32.2 metric tons ha-1)
and tomato paste production (92.9% paste return by tomato fruits weight) due to its ancient peculiar adaptability to cultivation in dry conditions, while âDatterinoâ, âCrovareseâ, and âSan Marzanoâ had 19, 16.7, 10.5 tons ha-1 of fruit yield and 85.7, 83.9, 76.2% of tomato paste return, respectively. Conversely, for the qualitative traits, such as total solids, soluble solids, titratable acidity, and ascorbic acid, âCrovareseâ showed the best results (9.1%, 6.6 °Brix, 0.93%, and 39.7 mg per 100 g of fresh weight, respectively). The two components extracted by the principal components analysis (PCA) explained 84.9% of the total variance in the morphological, quantitative and qualitative traits and the dendrogram
obtained by hierarchical cluster analysis allowed to divide the cultivars into three groups. Our findings highlighted that local tomato cultivars, intercropped with maize, can be cultivated adopting only few sustainable field operations and with no irrigation
The promising ability of Humulus lupulus L. Iso-Îą-acids vs. diabetes, inflammation, and metabolic syndrome: A systematic review
For centuries, natural medicines have represented the only option for treating human diseases and, nowadays, plant phytochemicals are considered as promising compounds to treat or prevent chronic conditions. Among them, hop flowers (Humulus lupulus L.), typically used in brewing industries to give the typical aroma and flavor to beer, have attracted particular attention for their health promoting properties. Several in vivo/vitro studies and human interventional trials have demonstrated the beneficial effects of these molecules on weight gain, lipid metabolism, glucose homeostasis, insulin sensitivities, and inflammation by acting on different targets. All these activities suggest a possible role of bitter hop acid in preventing metabolic syndrome and its related diseases. A systematic quest on PubMed and Scopus databases was performed to identify pre-clinical and clinical studies focusing on this topic. This systematic review summarizes the results obtained by different cell lines, animal models, and human interventional trials to propose iso-Îą-acids as medical nutrition therapy to treat or prevent metabolic syndrome and its related disorders as diabetes, dislipidemia inflammation, etc
New Insight on the Bioactivity of Solanum aethiopicum Linn. Growing in Basilicata Region (Italy): Phytochemical Characterization, Liposomal Incorporation, and Antioxidant Effects
Food extractâs biological effect and its improvement using nanotechnologies is one of the challenges of the last and the future decades; for this reason, the antioxidant effect of scarlet eggplant extract liposomal incorporation was investigated. Scarlet eggplant (Solanum aethiopicum L.) is a member of the Solanaceae family, and it is one of the most consumed vegetables in tropical Africa and south of Italy. This study investigated the antioxidant activity and the phytochemical composition of S. aethiopicum grown in the Basilicata Region for the first time. The whole fruit, peel, and pulp were subjected to ethanolic exhaustive maceration extraction, and all extracts were investigated. The HPLC-DAD analysis revealed the presence of ten phenolic compounds, including hydroxycinnamic acids, flavanones, flavanols, and four carotenoids (one xanthophyll and three carotenes). The peel extract was the most promising, active, and the richest in specialized metabolites; hence, it was tested on HepG2 cell lines and incorporated into liposomes. The nanoincorporation enhanced the peel extractâs antioxidant activity, resulting in a reduction of the concentration used. Furthermore, the extract improved the expression of endogenous antioxidants, such as ABCG2, CAT, and NQO1, presumably through the Nrf2 pathway
Capsicum annuum L. cv Senise: from food to liposomes with high-value antioxidant potential
Different factors increase the production of free radicals in human body altering the homeostasis (Flora, S. 2007). The increase in free radicals resulting in the oxidative stress, is involved in the etiology of several major human diseases such as diabetes, cardiovascular diseases, cancer, and neuronal disorders. Specialized metabolites extracted from plant species are able to neutralize free radicals by carrying out numerous biological activities (Zhang, H. et al. 2016). Among all, Capsicum annuum L. cultivar Senise, belonging to the Solanaceae family, is a precious source of health promoting compounds. In fact, few studies have investigated the biological activity and chemical composition of this pepper cultivar (Loizzo, M.R. et al. 2013; Speranza, G. et al. 2019). The âred gold of the Basilicata regionâ is a sweet pepper traditionally sun-dried and eaten fried (âcruschi peppersâ) or powdered and used as a spice in the Basilicata region (Italy). As far as we are aware, this is the first study on the biological activity of C. annuum cv Senise in cells or incorporated in a vesicular carrier system. The purpose of this study was the evaluation of the antioxidant activity on HepG2 cell line used as cell model as well as the investigation of the phytochemical profile of C. annuum. Furthermore, the molecular signalling pathways involved in the antioxidant activity of the extract were assessed (Sinisgalli, C. et al. 2020)
Enhancement of Yield, Phytochemical Content and Biological Activity of a Leafy Vegetable (Beta vulgaris L. var. cycla) by Using Organic Amendments as an Alternative to Chemical Fertilizer
This study evaluates the effect of a chemical fertilizer (ammonium nitrate), a compost (vermicompost from cattle manure) and two biochars (from vine prunings and wood chips, respectively), applied to the soil alone or in mixture, on the yield, phytochemical content and biological activity of Beta vulgaris L. var. cycla (Swiss chard). The respective treatments, each replicated four times, were arranged according to a completely randomized block design. Results showed that vermicompost, both alone and in mixture with vine pruning biochar, significantly increased yield parameters (plant height and leaf area) and yield over the untreated soil and the biochars alone, similar to ammonium nitrate. Moreover, vermicompost, both alone and in mixture, respectively, with the two biochars, determined lower total N and NO3− contents than ammonium nitrate, both alone and in mixture, respectively, with the two biochars. In particular, NO3− content was within the safe thresholds fixed for leafy vegetables by the European Commission to prevent any adverse implication on human health from dietary NO3− exposure. The biochars alone resulted in very low yield and leaf total N content, likely due to a limited release of N for plant uptake, also evidenced by the undetectable NO3− leaf content, similarly shown by plants grown in untreated soil. Vermicompost, alone or in mixture, respectively, with the two biochars, increased the content of specialized metabolites, with a positive effect on antioxidant activity. The organic amendments, particularly compost, could be an alternative to chemical fertilizers to reach a trade-off between yield, nutritional and health qualities in Swiss chard, meeting the needs of farmers and consumers as well as the targets for sustainable food production
The Production of Îł-Aminobutyric Acid from Free and Immobilized Cells of Levilactobacillus brevis Cultivated in Anaerobic and Aerobic Conditions
Îł-aminobutyric acid (GABA) has several beneficial effects on human health. GABA may
be produced via chemical synthesis or through microbial metabolism, and Levilactobacillus brevis
is recognized as a GABA-producing species. In this study, 11 Lvb. brevis strains were screened for
GABA production, and the best producers were selected to verify the effect of aerobic (AE) and
respiratory (RS) cultivations on growth parameters, biomass, and GABA accumulation. Lvb. brevis
LB12 was then used to evaluate the combined effect of the incubation atmosphere (anaerobiosis
vs. aerobiosis), cell protection (free vs. immobilized cells), and cell recycling (fresh vs. starved
cells) on GABA production. Glutamate (GLU) consumption and GABA accumulation were detected
by Thin-layer Chromatography (TLC) and RP-HPLC analyses. The ability to produce GABA was
widespread among the strains. AE and RS growth improved biomass production, but oxygen
availability impaired GLU to GABA conversion, and the anaerobically growing cells had the highest
GABA productivity. Immobilized strains had lower efficiency in both GLU uptake and conversion
compared to free cells, probably due to the poor diffusion in alginate beads. The use of resting cells
allowed further GABA production without the cultivation step, but cell activity was exhausted after
three cycles of reutilization. Lvb. brevis LB12 is an excellent GABA producer, and AE cultivation can
be exploited to improve the final cell density; however, the conditions for boosting GLU to GABA
conversion and cell regeneration need to be further investigated
The healing bitterness of Gentiana lutea L., phytochemistry and biological activities: A systematic review
Over many years, natural products have been a source of healing agents and have exhibited beneficial uses for treating human diseases. The Gentiana genus is the biggest genus in the Gentianaceae, with over 400 species distributed mainly in alpine zones of temperate countries around the world. Plants in the Gentiana genus have historically been used to treat a wide range of diseases. Still, only in the last years has particular attention been paid to the biological activities of Gentiana lutea Linn., also known as yellow Gentian or bitterwort. Several in vitro/vivo investigations and human interventional trials have demonstrated the promising activity of G. lutea extracts against oxidative stress, microbial infections, inflammation, obesity, atherosclerosis, etc.. A systematic approach was performed using Pubmed and Scopus databases to update G. lutea chemistry and activity. Specifically, this systematic review synthesized the major specialized bitter metabolites and the biological activity data obtained from different cell lines, animal models, and human interventional trials. This review aims to the exaltation of G. lutea as a source of bioactive compounds that can prevent and treat several human illnesses
An Insight into <i>Citrus medica</i> Linn.: A Systematic Review on Phytochemical Profile and Biological Activities
Plant species are a reservoir of natural compounds that can potentially be used to treat different diseases. Citrus medica Linn. belonging to the Rutaceae family, has been used for centuries in medicine for its antioxidant, anti-inflammatory, antimicrobial, antiviral, and antihyperglycemic properties. These activities are ascribable not only to the presence of health-promoting macronutrients and micronutrients, such as carbohydrates, minerals, amino acids, and vitamins, but also to specialized metabolites, such as flavonoids (apigenin, hesperetin, hesperidin, naringin, naringenin, rutin, quercetin, and diosmin), coumarins (citropten, scoparone, and bergapten), terpenes (limonene, Îł-terpinene, limonin, and nomilin), and phenolic acids (p-coumaric acid, trans-ferulic acid, and chlorogenic acid). In recent years, particular attention has been focused on the antioxidant, anti-inflammatory, antimicrobial activity, antidiabetic, anticancer, and neuroprotective activity of C. medica. However, although many studies have reported this speciesâ chemical and biological properties, the literature has never been analyzed via a systematic approach. For this reason, using PubMed and Scopus as databases, we performed a systematic review of C. medicaâs chemical composition and biological properties to inspire new research approaches and increase its curative application
β3-Adrenoceptor as a new player in the sympathetic regulation of the renal acidâbase homeostasis
Efferent sympathetic nerve fibers regulate several renal functions activating norepinephrine receptors on tubular epithelial cells. Of the beta-adrenoceptors (β-ARs), we previously demonstrated the renal expression of β3-AR in the thick ascending limb (TAL), the distal convoluted tubule (DCT), and the collecting duct (CD), where it participates in salt and water reabsorption. Here, for the first time, we reported β3-AR expression in the CD intercalated cells (ICCs), where it regulates acidâbase homeostasis. Co-localization of β3-AR with either proton pump H+-ATPase or Clâ/HCO3â exchanger pendrin revealed β3-AR expression in type A, type B, non-A, and non-B ICCs in the mouse kidney. We aimed to unveil the possible regulatory role of β3-AR in renal acidâbase homeostasis, in particular in modulating the expression, subcellular localization, and activity of the renal H+-ATPase, a key player in this process. The abundance of H+-ATPase was significantly decreased in the kidneys of β3-ARâ/â compared with those of β3-AR+/+ mice. In particular, H+-ATPase reduction was observed not only in the CD but also in the TAL and DCT, which contribute to acidâbase transport in the kidney. Interestingly, we found that in in vivo, the absence of β3-AR reduced the kidneysâ ability to excrete excess proton in the urine during an acid challenge. Using ex vivo stimulation of mouse kidney slices, we proved that the β3-AR activation promoted H+-ATPase apical expression in the epithelial cells of β3-AR-expressing nephron segments, and this was prevented by β3-AR antagonism or PKA inhibition. Moreover, we assessed the effect of β3-AR stimulation on H+-ATPase activity by measuring the intracellular pH recovery after an acid load in β3-AR-expressing mouse renal cells. Importantly, β3-AR agonism induced a 2.5-fold increase in H+-ATPase activity, and this effect was effectively prevented by β3-AR antagonism or by inhibiting either H+-ATPase or PKA. Of note, in urine samples from patients treated with a β3-AR agonist, we found that β3-AR stimulation increased the urinary excretion of H+-ATPase, likely indicating its apical accumulation in tubular cells. These findings demonstrate that β3-AR activity positively regulates the expression, plasma membrane localization, and activity of H+-ATPase, elucidating a novel physiological role of β3-AR in the sympathetic control of renal acidâbase homeostasis