Еffect of the essential oil of <i>Satureja montana</i> L. on the growth of cultures of conditionally pathogenic microorganisms

Introduction. Essential oils contain antimicrobial components that are highly active against a wide range of microorganisms. Essential oils are natural, environmentally safe, low-toxic substances with a minimal list of side effects; no antimicrobial resistance is formed to them. The aim of the research was to study the influence of the essential oil of Satureja montana L., growing in the Crimea, on the growth of cultures of opportunistic microorganisms. Materials and methods. The short-term effect of savory oil on the growth of referenсе strains of microorganisms was studied in accordance with the European Standard for determining the rate of inactivation of microorganisms by the test substance (1997). To study the long-term effect of savory oil on clinical isolates of Staphylococcus aureus, we used the method of dilutions in a liquid medium, followed by measurement of the optical density of growth of the suspension culture biomass. The effect of savory oil on the formation of biofilms by clinical isolates of S. aureus was also studied. Results. Whole savory oil and its dilutions of 1 : 10 and 1 : 100 with short-term action (1060 min) completely suppressed the growth of referenсе strains of bacteria; growth of the referenсе strain Candida albicans CCM 885 was inhibited only by whole oil and a 1 : 10 dilution, while a 1 : 100 dilution had a bacteriostatic effect. Dilutions of essential oil 1 : 100 and 1 : 1000 had a pronounced antibacterial effect on the suspension culture of clinical isolates of S. aureus. Savory oil also inhibited biofilm formation by 11 isolates S. aureus. Conclusion. The essential oil of Satureja montana L. exhibits a pronounced antimicrobial effect against referenсе strains of S. aureus ATCC 25923, Escherichia coli ATCC 25922 and fungi C. albicans CCM 885. The antibacterial effect of this essential oil on clinical isolates of S. aureus allows us to offer it as a component of combined preparations for the treatment of infections caused by antibiotic-resistant strains of staphylococcus

A Comparison of the Capacity of Mesenchymal Stromal Cells for Cartilage Regeneration Depending on Collagen-Based Injectable Biomimetic Scaffold Type

Mesenchymal stromal cells (MSCs) have shown a high potential for cartilage repair. Collagen-based scaffolds are used to deliver and retain cells at the site of cartilage damage. The aim of the work was a comparative analysis of the capacity of the MSCs from human adipose tissue to differentiate into chondrocytes in vitro and to stimulate the regeneration of articular cartilage in an experimental model of rabbit knee osteoarthrosis when cultured on microheterogenic collagen-based hydrogel (MCH) and the microparticles of decellularized porcine articular cartilage (DPC). The morphology of samples was evaluated using scanning electron microscopy and histological staining methods. On the surface of the DPC, the cells were distributed more uniformly than on the MCH surface. On day 28, the cells cultured on the DPC produced glycosaminoglycans more intensely compared to the MCH with the synthesis of collagen type II. However, in the experimental model of osteoarthrosis, the stimulation of the cartilage regeneration was more effective when the MSCs were administered to the MCH carrier. The present study demonstrates the way to regulate the action of the MSCs in the area of cartilage regeneration: the MCH is more conducive to stimulating cartilage repair by the MSCs, while the DPC is an inducer for a formation of a cartilage-like tissue by the MSCs in vitro

Cryostructuring of Polymeric Systems: 63. Synthesis of Two Chemically Tanned Gelatin-Based Cryostructurates and Evaluation of Their Potential as Scaffolds for Culturing of Mammalian Cells

Various gelatin-containing gel materials are used as scaffolds for animal and human cell culturing within the fields of cell technologies and tissue engineering. Cryostructuring is a promising technique for the preparation of efficient macroporous scaffolds in biomedical applications. In the current study, two new gelatin-based cryostructurates were synthesized, their physicochemical properties and microstructure were evaluated, and their ability to serve as biocompatible scaffolds for mammalian cells culturing was tested. The preparation procedure included the dissolution of Type A gelatin in water, the addition of urea to inhibit self-gelation, the freezing of such a solution, ice sublimation in vacuo, and urea extraction with ethanol from the freeze-dried matter followed by its cross-linking in an ethanol medium with either carbodiimide or glyoxal. It was shown that in the former case, a denser cross-linked polymer phase was formed, while in the latter case, the macropores in the resultant biopolymer material were wider. The subsequent biotesting of these scaffolds demonstrated their biocompatibility for human mesenchymal stromal cells and HepG2 cells during subcutaneous implantation in rats. Albumin secretion and urea synthesis by HepG2 cells confirmed the possibility of using gelatin cryostructurates for liver tissue engineering