MOESM3 of RNA-Seq based transcriptome of whole blood from immunocompetent pigs (Sus scrofa) experimentally infected with Mycoplasma suis strain Illinois

Additional file 3. Relative expression (RE) of differentially expressed (DE) in pigs infected with Mycoplasma suis validated by qRT-PCR. Relative expression profile (GAPDH as reference control gene) of DE genes identified in the M. suis-infected pigs compared to the control group (non-infected). Validated genes are: ISG15 ubiquitin-like modifier (ISG15), interleukin 22 receptor, alpha 2 (IL22RA2), CD274 molecule (CD274), BCL2-like 14 (apoptosis facilitator) (BCL2), chemokine (C-C motif) receptor 5 (CCR5), CD180 molecule (CD180), allograft inflammatory factor 1 (AIF1), interleukin 15 (IL15), protein tyrosine phosphatase, receptor type, O (PTPRO), and Toll-like receptor 8 (TLR8). Black dots in each graphic represent the RE of a respective gene for each pig; blue vertical lines represent the standard deviation in each group. * p-value ≤ 0.1, and ** p-value ≤ 0.05

MOESM2 of RNA-Seq based transcriptome of whole blood from immunocompetent pigs (Sus scrofa) experimentally infected with Mycoplasma suis strain Illinois

Additional file 2. Results of RNA-Seq analyses. List of differentially expressed (DE) genes identified in whole blood of Mycoplasma suis-infected pigs (when compared to sham-infected group) separated by all three methods of RNA-Seq analyses (DESeq2, edgeR, Cufflinks). First tab describes all DE genes (p-value ≤ 0.1) that were detected in all three methods, in two methods, followed by each individual method. The following tabs report the statistics of each evaluated gene for each method. Highlighted in grey are statistically significant DE genes (dark grey: p-value ≤ 0.05; light grey p-value ≤ 0.1)

MOESM1 of RNA-Seq based transcriptome of whole blood from immunocompetent pigs (Sus scrofa) experimentally infected with Mycoplasma suis strain Illinois

Additional file 1. Results of Mycoplasma suis microbead immunoassay (MIA). Median Fluorescence Intensity (MFI) of Mycoplasma suis-infected and sham-inoculated pigs as detected in serum samples collected along the experiment. Serum antibodies were measured using recombinant GrpE (rGrpE) protein in a MIA [30]. Sham-inoculated group (control group): pig #1, pig #2 and pig #3. M. suis-inoculated group (infected group): pig #5, pig #6, pig #7. Day 0 corresponds to inoculation day. MFI cut-off was 74.07. It is important to note that a high cut-off may have been encountered due to the low number of animals to determine a cut-off, which increases the standard deviation. MFI values in bold correspond to positive results based on cut-off values for each group. ND: not done

MOESM4 of RNA-Seq based transcriptome of whole blood from immunocompetent pigs (Sus scrofa) experimentally infected with Mycoplasma suis strain Illinois

Additional file 4. Gene ontology (GO) categories of all 55 differentially expressed (DE) genes of Mycoplasma suis infected pigs. First tab describes raw results of GO analysis, with all DE genes and their correspondent GO categories (and functions). Second tab shows the identified functions of the “biological process” category of all 55 DE genes and the number of reported genes for each function. Functional categories with the largest number of genes are highlighted in yellow. GO analysis was performed using Blast2Go software [39]

Additional file 2: Table S2. of Co-infection of sexually transmitted pathogens and Human Papillomavirus in cervical samples of women of Brazil

Primers and probes used in qPCR to quantify DNA of U. urealyticum and U. parvum in cervical samples. (DOC 50 kb