52 research outputs found
Glitch Collective
Extent : 15 minutes This event was curated by Tristan Louth Robins as part of the Tyndall Series ; the event was promoted by Luke Altmann
Photocurable antimicrobial silk-based hydrogels for corneal repair
Corneal transplantation is the current gold standard treatment to restore visual acuity to patients with severe corneal diseases and injuries. Due to severe donor tissue shortage, efforts to develop a corneal equivalent have been made but the challenge remains unmet. Another issue of concern in ocular surgery is the difficult instillation and fast drainage of antibiotic ocular eye drops as bacterial infections can jeopardize implant success by delaying or impairing tissue healing. In this study, we developed antimicrobial silkâbased hydrogels that have the potential to be photoactivated in situ, fully adapting to the corneal injury shape. Gentamicinâloaded methacrylatedâsilk (SilkMA) hydrogels were prepared within minutes using low UV intensity (3Â mW/cm(2)). SilkMA gels provided a Young's modulus between 21 and 79âkPa together with a light transmittance spectrum and water content (83%â90%) similar to the human cornea. Polymer concentration (15%â25%) was found to offer a tool for tailoring the physical properties of the hydrogels. We confirmed that the methacrylation did not affect the material's in vitro degradation and biocompatibility by observing fibroblast adhesion and proliferation. Importantly, agar diffusion tests showed that the synthesized hydrogels were able to inhibit Staphylococcus aureus and Pseudomonas aeruginosa growth for 72âh. These characteristics along with their injectability and viscoelasticity demonstrate the potential of SilkMA hydrogels to be applied in several soft tissue engineering fields. As such, for the first time we demonstrate the potential of photocurable antimicrobial SilkMA hydrogels as a novel biomaterial to facilitate corneal regeneration
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