Effects of incorporation levels of Pueraria phaseoloides leaf flour on carcass characteristics and chemical composition of meat from local rabbit (Oryctolagus cuniculus) in South-East Gabon

The objective of this study was to contribute in improving knowledge on rabbit feeding in Gabon. This work was conducted with the aim of studying carcass characteristics and chemical composition of meat from rabbit induced by feeds containing P. phaseoloides. To that effect, 20 young female rabbits from local breed, weighing averagely 611 ± 33.20 g and aged about 6 weeks were randomly distributed in 4 groups of 5 animals each. The groups R0, R15, R20 and R25 were fed rations containing respectively 0%, 15%, 20% and 25% of P. phaseoloides. At the end of the trial, animals were slaughtered then eviscerated in order to evaluate carcass characteristics. Muscles were taken, ground and mixed for chemical analysis. No matter the ration, carcass characteristics showed no significant difference (p > 0.05). Highest protein contents in meat were recorded in animals from R15 (23.90% DM) and R20 (22.46% DM). The lowest fat content (5.41% DM) was that of animals from R25, followed by that from R20 (6.67% DM). Based on these results obtained, the ration containing 20% Pueraria phaseolides (R20) could be recommended

Chemical composition, antioxidant and antimicrobial activities of stem barks of Englerina gabonensis Engler and Sterculia tragacantha Lindl from Gabon

Aim of this work was to evaluate the phytochemical constituents, antioxidant and antimicrobial potential of water-acetone, water-ethanol and water extracts of Englerina gabonensis and Sterculia tragacantha.Presence of phenols was evaluated to estimate the effects of plants on microbial diseases. Water-acetone, water-ethanol and water extracts were examined for antioxidant activities. All plant extracts were tested against six reference strains, eleven clinical isolates and two fungal strains.Phenolic content were highest in the water-acetone and water-ethanol extracts from Englerinagabonensis in comparison with Sterculia tragacantha.The AAI (Antioxidant Activity Index)of water-acetone and water-ethanol extracts of Englerina gabunensis are superiors with 2. Plant extracts of Sterculia tragacantha show weak antioxidant activity (AAI < 0.5).The aqueous extract of Englerina gabonensis has a bactericidal effect on Salmonella Spp. Water-ethanol extract is bactericidal on Bacillus cereus LMG 13569 BHI, Salmonella Sppand Neisseria meningitides. Water-acetone extract presents a bactericidal activity on Enterococcus faecalis103907 CIP, Escherichi coli, Staphylococcus aureus, Acinetobacter baumanniiand Neisseria meningitides.Our results suggest that Englerina gabonensis extracts contain greater antioxidant and antimicrobial properties than Sterculia tragacantha extracts

Bacteriological profile and antibiotic sensitivity of pus isolates in patients at Regional Hospital Center of Franceville in Gabon

Background:Bacterial resistance to antibiotics is a public health problem, it is a threat to the future. It compromises the effectiveness of antibiotic treatment and is constantly increasing worldwide. This work has been highlighted by the profile and sensitivity of pyogenic bacteria isolated at Franceville Regional Hospital Center. Methods:Effective antibiotic strategy, suppurations from the wounds of 530 patients were collected and analyzed. Biochemical identification of isolated bacterial strains was performed with Api 10s galleries and verified using Vitek-2 system. The Kirby-Bauer technic was used to perform the antibiogram. Five antibiotics were first tested in a simple way and then combined in pairs. Results:360 samples showed bacterial strains and 170 were sterile cultures. 360 microorganisms were isolated, of which 230 strains came from hospitalization surgery, 110 from minor surgery and 20 from medicine.The bacterial families identified are Enterobacteriaceae, Moraxellaceae, Pseudomonadaceae, Streptococcaceae and Xanthomonadaceae. Enterobacterizceae is the most common family with 64.69% and has revealed 7 genera Citrobacter, Enterobacter, Escherichia, Klebsiella, Serratia, Yersinia and Proteus 65% antibiotic sensitivity of simple antibiotics showed the best sensitivities with Ciprofloxacin and Ofloxacin a resistance rate 31.25%, 100% unlike Amoxicillin and Metronidazole. Ceftazidim was not very active on bacterial strains with a resistance rate of 93.75%. The interactions between antibiotics combined gave antagonistic, synergistic, indifferent and additive effects. The indifferent effect was much more observed (44 cases), in contrast to the additive effect which represented only 23 cases. The antagonic and synergistic effects were reported in 14 cases. Combined Amoxicillin and Metronidazole, Ceftazidim antibiotics showed good activity on bacterial strains, with good synergy and additive effect, or were not combined. Conclusion:The emergence of bacteria is major global problem multi-resistance. The rationalization of the prescription of antibiotics, especially Ofloxacin and Ciprofloxacin, and the optimization of bacteriological prescriptions are desirable in surgical services. However, rigor in dispensing pharmacies and the rational use of these antibiotics are essential to avoid the emergence of resistance phenomena

Phytochemical studies, antiangiogenic, anti-inflammatory and antioxidant activities of Scyphocephalium ochocoa Warb. (Myristicaceae), medicinal plant from Gabon

Abstract Background The search for new anti-cancer molecules is one of the main concerns of oncology researchers. Scyphocephalium ochocoa is a plant of Myristicaceae family, used in traditional medicine against inflammatory diseases and several types of cancer. It is well established that free radicals, chronic inflammation and angiogenesis play an important role in initiation, tumor progression and metastasis formation. The aim of this study was to carry out a phytochemical screening, to determine the phenolic compounds content, to investigate the antiangiogenic, anti-inflammatory and antioxidant activities of water, water-ethanol and ethanol extracts of S. ochocoa. Methods Phytochemical screening and determination of phenolic compounds content were performed using standard methods. Antiangiogenic activity was assessed using chick chorioallantoic membrane (CAM) model and Drabkin test. Anti-inflammatory activity was estimated by protein denaturation and erythrocyte membrane stabilization method. Finally the antioxidant activity was appreciated by DPPH radical inhibition and phosphomolybdenum assay. Results The results of phytochemical studies show that extracts of bark of S. ochocoa are rich in polyphenols, tannins, flavonoids, proantocyanidins, saponosides, flavonols, flavanonols, sterol and triterpenes. The water extract showed good antiangiogenic activity (IC50 = 1.153 μg/mL). Strong anti-inflammatory activity was observed with all extracts, IC50 ranging from 34.775 ± 2.543 μg/mL to 74.577 ± 3.456 μg/mL for protein denaturation inhibition test and IC50 values ranging from 36.793 ± 0.529 μg/mL at 48.912 ± 0.957 μg/mL for antihemolytic activity. In addition, all extracts showed good antioxidant activity marked by a strong inhibition of the DPPH radical (IC50 ranging from 4.969 ± 0.263 μg/mL to 16.188 ± 0.336 μg/mL and AAI ranging from 3.090 ± 0.065 to 10.080 ± 0.517) and by greater total antioxidant capacity (with contents ranging from 37.654 ± 0.995 to 131.302 ± 1.102 VtCE (mg)/g dry extract). Conclusion Ultimately, these results could justify the use of S. ochocoa extracts in traditional medicine in the treatment of diseases related to angiogenesis and cancer, inflammatory diseases and diseases due to oxidative stress. A phyto-product with such a pharmacological profile could be a good candidate for the development of anticancer

Correction to: Phytochemical studies, antiangiogenic, antiinflammatory and antioxidant activities of Scyphocephalium ochocoa Warb. (Myristicaceae), medicinal plant from Gabon

Following the publication of this article [1], the authors noticed that Figs. 1, 2 and 3 were in the incorrect order and thus had incorrect captions. The images that were incorrectly published as Figs. 1, 2 and 3 should have been published as Figs. 2, 3 and 1 respectively

Structure elucidation of a new lanostane triterpene from Gabonese Ganoderma orbiforme fruiting bodies

International audienceThe structure and complete nuclear magnetic resonance (NMR) assignments of orbifomitellic acid, a novel lanostane triterpene isolated from the fruiting bodies of a Gabonese Ganoderma orbiforme (Polyporaceae), are reported. Within the vast catalogue of lanostanes documented from Ganoderma spp., orbifomitellic acid is the first disclosing a -COOH group at C-4

Toxicity, Antibacterial, and Phytochemical Analyses of Antrocaryon klaineanum Pierre Extracts

Medicinal plants are traditionally used in Gabon to treat several types of illnesses. The study’s purpose was to determine the toxic, antibacterial, and anti-inflammatory effects of Antrocaryon klaineanum Pierre extracts and to characterize their phytochemical compounds. Toxicity was evaluated on frog tadpoles (Phrynobatrachus africanus Hallowell). The microorganism susceptibility test was performed by the diffusion method, while minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were evaluated using the microdilution technique. Anti-inflammatory activity was tested through protein denaturation and membrane stabilization methods. Chromatography and molecular network techniques were used to characterize chemical compounds. The lethality test showed that the lethal concentration (LC50) increased from 110.03 ± 1.25 to 15.86 ± 2.21 μg/mL after 24 and 96 hours of exposure. In tadpoles exposed to 7.81 μg/mL extract, the first mortalities (12.5%) were observed on the fifth day of exposure. A relative decrease in mature erythrocytes exposed to plant extracts was observed. The antibacterial activity shows that the Ak F2, Ak F3, and Ak F4 fractions (from the water-ethanol crude extract) gave the greatest antibacterial activities compared to the other extracts. The water, water-acetone, and water-ethanol extracts showed good inhibition of denaturation. The haemolysis test shows that the extracts exhibited good anti-inflammatory activities. Phytochemical characterisation revealed four major compounds, including monogallate epicatechin and hydroxy-ergostadian. The molecular network revealed five main clusters. Our study shows that A. klaineanum Pierre could be a promising natural product for the isolation of molecules with potential biological activities