Les effets cationiques et secretoires des secretagogues d'insuline dans les îlots pancréatiques des rats perifusés

Doctorat en sciences médicalesinfo:eu-repo/semantics/nonPublishe

Etude du taux de survie d’implants dentaires chez les patients diabétiques de plus de 60 ans

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Effect of the meglitinide analog S21403 on cationic fluxes and insulin release in perifused rat pancreatic islets exposed to a high concentration of D-glucose

The effect of the meglitinide analog S21403 (10 μM) upon 86Rb and 45Ca outflow and insulin release was investigated in perifused rat islets exposed to a high concentration of D-glucose (16.7 mM) in order to simulate the situation found in diabetic patients. Under these conditions, S21403 provoked a rapid, sustained and rapidly reversible increase in 86Rb outflow, 45Ca efflux and insulin release. These effects were suppressed or reversed when the experiments were conducted in the absence of extracellular Ca2+. They support the view that S21043 could be used as a novel insulinotropic tool in the treatment of non-insulin-dependent diabetes mellitus, the cationic and secretory responses to the drug displaying a favourable time course for prompt and not unduly prolonged activation of islet B-cells.SCOPUS: ar.jinfo:eu-repo/semantics/publishe

Effect of a novel non-sulfonylurea antidiabetic agent on cationic fluxes and insulin release in rat pancreatic islets

info:eu-repo/semantics/published57th Annual Meeting of the American Diabetes Association, Boston, USA, 22/6/1997.(supplem. 1

Effects of α-D- And β-L-glucose pentaacetate on effluent radioactivity from pancreatic islets labelled with [32P]phosphate and myo-[2-3H]inositol

The anomers of both D-glucose pentaacetate and L-glucose pentaacetate were recently found to display insulinotropicpotential. In order to progress in understanding the mode of action of these esters in islet cells, we have now investigated whether they mimic the effect of nutrient secretagogues to cause a phosphate flush and activation of phospholipase C in isolated islets. For this purpose, rat pancreatic islets were prelabelled with either [32P]orthophosphate or myo-[2-3H]inositol and placed in a perifusion chamber. In the absence of any other exogenous nutrient, the administration of α-D-glucose pentaacetate (1.7 mM) from 46 to 70 min of perifusion increased, after an initial transient fall, both 32P and 3H fractional outflow rates and stimulated insulin release from the perifused islets. No secondary rise in either 32P or 3H outflow and no sizeable stimulation of insulin release was observed, however, in response to β-L-glucose pentaacetate (also 1.7 mM). These findings are consistent with the view that the insulinotropic action of α-D-glucose pentaacetate entails a nutrient-like component leading to the occurrence of both a phosphate flush and hydrolysis of phosphoinositides. This is not the case, however, for β-L-glucose pentaacetate. The latter ester might act directly on a yet unidentified receptor, the early secretory response to α-D-glucose pentaacetate also apparently involving such a direct effect of the ester itself.SCOPUS: ar.jinfo:eu-repo/semantics/publishe

Noninvasive imaging of pancreatic Β cells

SCOPUS: re.jinfo:eu-repo/semantics/publishe

Dual effect of nutrients on insulin release from Ca2+-deprived and depolarized rat pancreatic islets exposed to diazoxide

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Effects of α-D and β-L-glucose pentaacetate on effluent radioactivity from pancreatic islets labelled with [32P]orthophosphate and myo-[2-3H]inositol

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Effects of polyethylene glycol, bovine serum albumin and an ionophoretic islet extract on glucose-stimulated insulin release from rat pancreatic islets

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Glucose-induced phosphate flush in rat pancreatic islets: role of volume sensitive anion channels

info:eu-repo/semantics/publishedComm. 67th Annual Meeting of the American Diabetes Association – Chicago (USA), 22-06-200