The conductive environment enhances gross motor function of girls with Rett syndrome. A pilot study

Introduction: Rett syndrome (RTT) is a neurological disorder usually associated with a mutation in the MECP2 gene. Conductive Education (CE) is an educational approach that has not yet been explored with regard to children with RTT. Objective: Assessing functional abilities of individuals with RTT due to CE intervention. Design: A single subject, AB design. Method: This study assessed the functional skills of three girls with RTT aged 3–5 years before and during participation in a CE programme. Results: Gross motor function improvements were observed at the end of the intervention period. Gross motor skills declined slightly in all participants over the summer holidays but improved again a few months after recommencement of the educational year. Conclusion: Replication of this study with more subjects is justified as is comparison with other educational methods. A home intervention programme should be constructed to prevent decline of skills over the summer vacation

Decrease in drug accumulation and in tumour aggressiveness marker expression in a fenretinide-induced resistant ovarianumour cell line

We investigated whether the efficacy of fenretinide (HPR) against ovarian tumours may be limited by induction of resistance. The human ovarian carcinoma cell line A2780, which is sensitive to a pharmacologically achievable HPR concentration (IC 50= 1 μM), became 10-fold more resistant after exposure to increasing HPR concentrations. The cells (A2780/HPR) did not show cross-resistance to the synthetic retinoid 6-[3-adamantyl-4-hydroxyphenyl]-2-naphthalene carboxylic acid (CD437) and were not sensitive, similarly to the parent line, to all- trans -retinoic acid, 13- cis -retinoic acid or N-(4-methoxyphenyl)retinamide. A2780/HPR cells showed, compared to parental cells, a 3-fold reduction in colony-forming ability in agar. The development of HPR resistance was associated with a marked increase in retinoic acid receptor β (RARβ) mRNA and protein levels, which decreased, together with drug resistance, after drug removal. The expression of cell surface molecules associated with tumour progression including HER-2, laminin receptor and β1 integrin was markedly reduced. The increase in the levels of reactive oxygen species is not involved in HPR-resistance because it was similar in parental and resistant cells. Conversely differences in pharmacokinetics may account for resistance because, in A2780/HPR cells, intracellular peak drug levels were 2 times lower than in A2780 cells and an as yet unidentified polar metabolite was present. These data suggest that acquired resistance to HPR is associated with changes in marker expression, suggestive of a more differentiated status and may be explained, at least in part, by reduced drug accumulation and increased metabolism. © 2001 Cancer Research Campaign http://www.bjcancer.co

Hashtags Functions in the Protests Across Brazil

In this article, we discuss the communicative functions of hashtags during a period of major social protests in Brazil. Drawing from a theoretical background of the use of Twitter and hashtags in protests and the functions of language, we extracted a sample of 46,090 hashtags from 2,321,249 tweets related to Brazilian protests in June 2013. We analyzed the hashtags through content analysis, focusing on functions, and co-occurrences. We also qualitatively analyzed a group of 500 most retweeted tweets to understand the users’ tagging behavior. Our results show how users appropriate tags to accomplish different effects on the narrative of the protests

Social determinants of content selection in the age of (mis)information

Despite the enthusiastic rhetoric about the so called \emph{collective intelligence}, conspiracy theories -- e.g. global warming induced by chemtrails or the link between vaccines and autism -- find on the Web a natural medium for their dissemination. Users preferentially consume information according to their system of beliefs and the strife within users of opposite narratives may result in heated debates. In this work we provide a genuine example of information consumption from a sample of 1.2 million of Facebook Italian users. We show by means of a thorough quantitative analysis that information supporting different worldviews -- i.e. scientific and conspiracist news -- are consumed in a comparable way by their respective users. Moreover, we measure the effect of the exposure to 4709 evidently false information (satirical version of conspiracy theses) and to 4502 debunking memes (information aiming at contrasting unsubstantiated rumors) of the most polarized users of conspiracy claims. We find that either contrasting or teasing consumers of conspiracy narratives increases their probability to interact again with unsubstantiated rumors.Comment: misinformation, collective narratives, crowd dynamics, information spreadin

Glycotope structures and intramolecular affinity factors of plant lectins for Tn/T antigens

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Analyzing Users' Activity in On-line Social Networks over Time through a Multi-Agent Framework

[EN] The number of people and organizations using online social networks as a new way of communication is continually increasing. Messages that users write in networks and their interactions with other users leave a digital trace that is recorded. In order to understand what is going on in these virtual environments, it is necessary systems that collect, process, and analyze the information generated. The majority of existing tools analyze information related to an online event once it has finished or in a specific point of time (i.e., without considering an in-depth analysis of the evolution of users activity during the event). They focus on an analysis based on statistics about the quantity of information generated in an event. In this article, we present a multi-agent system that automates the process of gathering data from users activity in social networks and performs an in-depth analysis of the evolution of social behavior at different levels of granularity in online events based on network theory metrics. We evaluated its functionality analyzing users activity in events on Twitter.This work is partially supported by the PROME-TEOII/2013/019, TIN2014-55206-R, TIN2015-65515-C4-1-R, H2020-ICT-2015-688095.Del Val Noguera, E.; Martínez, C.; Botti, V. (2016). Analyzing Users' Activity in On-line Social Networks over Time through a Multi-Agent Framework. Soft Computing. 20(11):4331-4345. https://doi.org/10.1007/s00500-016-2301-0S433143452011Ahn Y-Y, Han S, Kwak H, Moon S, Jeong H (2007) Analysis of topological characteristics of huge online social networking services. In: Proceedings of the 16th WWW, pp 835–844Bastiaensens S, Vandebosch H, Poels K, Cleemput KV, DeSmet A, Bourdeaudhuij ID (2014) Cyberbullying on social network sites. an experimental study into behavioural intentions to help the victim or reinforce the bully. Comput Hum Behav 31:259–271Benevenuto F, Rodrigues T, Cha M, Almeida V (2009) Characterizing user behavior in online social networks. In: Proceedings of the 9th ACM SIGCOMM conference on Internet measurement conference. ACM, pp 49–62Borge-Holthoefer J, Rivero A, García I, Cauhé E, Ferrer A, Ferrer D, Francos D, Iñiguez D, Pérez MP, Ruiz G et al (2011) Structural and dynamical patterns on online social networks: the Spanish may 15th movement as a case study. PLoS One 6(8):e23883Borondo J, Morales AJ, Losada JC, Benito RM (2013) Characterizing and modeling an electoral campaign in the context of Twitter: 2011 Spanish presidential election as a case studyCatanese SA, De Meo P, Ferrara E, Fiumara G, Provetti A (2011) Crawling facebook for social network analysis purposes. In: Proceedings of the international conference on web intelligence, mining and semantics. ACM, p 52Cha M, Mislove A, Gummadi KP (2009) A measurement-driven analysis of information propagation in the flickr social network. In: Proceedings of the 18th international conference on World Wide Web. ACM, pp 721–730del Val E, Martínez C, Botti V (2015a) A multi-agent framework for the analysis of users behavior over time in on-line social networks. In: 10th International conference on soft computing models in industrial and environmental applications. Springer, Berlin, pp 191–201del Val E, Rebollo M, Botti V (2015b) Does the type of event influence how user interactions evolve on twitter? PLOS One 10(5):e0124049Eurostat (2016a) Internet use statistics—individuals. http://ec.europa.eu/eurostat/statistics-explained/index.php/Internet_use_statistics_-_individuals . Accessed 29 April 2016Eurostat (2016b) Social media—statistics on the use by enterprises. http://ec.europa.eu/eurostat/statistics-explained/index.php/Social_media_-_statistics_on_the_use_by_enterprises#Further_Eurostat_information . Accessed 29 April 2016García Fornes AM, Rodrigo Solaz M, Terrasa Barrena AM, Inglada J, Javier V, Jorge Cano J, Mulet Mengual L, Palomares Chust A, Búrdalo Rapa LA, Giret Boggino AS et al (2015) Magentix 2 user’s manualGolbeck J, Robles C, Turner K (2011) Predicting personality with social media. In: CHI’11, pp 253–262Guimerà R, Llorente A, Moro E, Sales-Pardo M (2012) Predicting human preferences using the block structure of complex social networks. PloS One 7(9):e44620Huberman BA, Romero DM, Wu F (2008) Social networks that matter: Twitter under the microscope. arXiv preprint arXiv:0812.1045Jamali M, Abolhassani H (2006) Different aspects of social network analysis. In: 2006 IEEE/WIC/ACM international conference on web intelligence (WI 2006 main conference proceedings)(WI’06). IEEE, pp 66–72Jiang Y, Jiang J (2014) Understanding social networks from a multiagent perspective. Parallel Distrib Syst IEEE Trans 25(10):2743–2759Kossinets G, Watts D (2006) Empirical analysis of an evolving social network. Science 311(5757):88–90Kumar R, Novak J, Tomkins A (2010) Structure and evolution of online social networks. In: Yu PS, Han J, Faloutsos C (eds) Link mining: models, algorithms, and applications. Springer, New York, pp 337–357Lazer D (2009) Life in the network: the coming age of computational social science. Science 323(5915):721–723Leskovec J, Adamic LA, Huberman BA (2007) The dynamics of viral marketing. ACM Trans Web 1(1):5Licoppe C, Smoreda Z (2005) Are social networks technologically embedded? How networks are changing today with changes in communication technology. Soc Netw 27(4):317–335Lotan G, Graeff E, Ananny M, Gaffney D, Pearce I, Boyd D (2011) The revolutions were tweeted: information flows during the 2011 tunisian and egyptian revolutions. Int J Commun 5:1375–1405Peña-López I, Congosto M, Aragón P (2013) Spanish indignados and the evolution of 15M: towards networked para-institutions. Big data: challenges and opportunities, pp 25–26Perliger A, Pedahzur A (2011) Social network analysis in the study of terrorism and political violence. PS Polit Sci Polit 44:45–50Romero DM, Galuba W, Asur S, Huberman BA (2011a) Influence and passivity in social media. In: Proceedings of the 20th WWW, pp 113–114Romero DM, Meeder B, Kleinberg J (2011b) Differences in the mechanics of information diffusion across topics: idioms, political hashtags, and complex contagion on Twitter. In: Proceedings of the 20th WWW, pp 695–704Stockman FN, Doreian P, (1997) Evolution of social networks: processes and principles. In: Doreian P, Stokman FN (eds) Evolution of social networks. Routledge, London, pp 233–250Traud AL, Mucha PJ, Porter MA (2012) Social structure of facebook networks. Phys A Stat Mech Its Appl 391(16):4165–4180Ugander J, Karrer B, Backstrom L, Marlow C (2011) The anatomy of the Facebook social graph. arXiv preprint arXiv:1111.4503Valero S, del Val E, Alemany J, Botti V (2015) Using magentix2 in smart-home environments. In: 10th International conference on soft computing models in industrial and environmental applications. Springer, Berlin, pp 27–37Wasserman S, Faust K (1994) Social network analysis: methods and applications. Cambridge University Press, CambridgeWersm (2015) How much data is generated every minute on social media? http://wersm.com/how-much-data-is-generated-every-minute-on-social-media/ . Accessed 29 April 201

Intravesical rAd-IFNα/Syn3 for Patients With High-Grade, Bacillus Calmette-Guerin-Refractory or Relapsed Non-Muscle-Invasive Bladder Cancer: A Phase II Randomized Study.

Purpose Many patients with high-risk non-muscle-invasive bladder cancer (NMIBC) are either refractory to bacillus Calmette-Guerin (BCG) treatment or may experience disease relapse. We assessed the efficacy and safety of recombinant adenovirus interferon alfa with Syn3 (rAd-IFNα/Syn3), a replication-deficient recombinant adenovirus gene transfer vector, for patients with high-grade (HG) BCG-refractory or relapsed NMIBC. Methods In this open-label, multicenter (n = 13), parallel-arm, phase II study ( ClinicalTrials.gov identifier: NCT01687244), 43 patients with HG BCG-refractory or relapsed NMIBC received intravesical rAd-IFNα/Syn3 (randomly assigned 1:1 to 1 × 10(11) viral particles (vp)/mL or 3 × 10(11) vp/mL). Patients who responded at months 3, 6, and 9 were retreated at months 4, 7, and 10. The primary end point was 12-month HG recurrence-free survival (RFS). All patients who received at least one dose were included in efficacy and safety analyses. Results Forty patients received rAd-IFNα/Syn3 (1 × 10(11) vp/mL, n = 21; 3 × 10(11) vp/mL, n = 19) between November 5, 2012, and April 8, 2015. Fourteen patients (35.0%; 90% CI, 22.6% to 49.2%) remained free of HG recurrence 12 months after initial treatment. Comparable 12-month HG RFS was noted for both doses. Of these 14 patients, two experienced recurrence at 21 and 28 months, respectively, after treatment initiation, and one died as a result of an upper tract tumor at 17 months without a recurrence. rAd-IFNα/Syn3 was well tolerated; no grade four or five adverse events (AEs) occurred, and no patient discontinued treatment because of an adverse event. The most frequently reported drug-related AEs were micturition urgency (n = 16; 40%), dysuria (n = 16; 40%), fatigue (n = 13; 32.5%), pollakiuria (n = 11; 28%), and hematuria and nocturia (n = 10 each; 25%). Conclusion rAd-IFNα/Syn3 was well tolerated. It demonstrated promising efficacy for patients with HG NMIBC after BCG therapy who were unable or unwilling to undergo radical cystectomy

Does \u2018bigger\u2019mean \u2018better\u2019? Pitfalls and shortcuts associated with big data for social research

\u2018Big data is here to stay.\u2019 This key statement has a double value: is an assumption as well as the reason why a theoretical reflection is needed. Furthermore, Big data is something that is gaining visibility and success in social sciences even, overcoming the division between humanities and computer sciences. In this contribution some considerations on the presence and the certain persistence of Big data as a socio-technical assemblage will be outlined. Therefore, the intriguing opportunities for social research linked to such interaction between practices and technological development will be developed. However, despite a promissory rhetoric, fostered by several scholars since the birth of Big data as a labelled concept, some risks are just around the corner. The claims for the methodological power of bigger and bigger datasets, as well as increasing speed in analysis and data collection, are creating a real hype in social research. Peculiar attention is needed in order to avoid some pitfalls. These risks will be analysed for what concerns the validity of the research results \u2018obtained through Big data. After a pars distruens, this contribution will conclude with a pars construens; assuming the previous critiques, a mixed methods research design approach will be described as a general proposal with the objective of stimulating a debate on the integration of Big data in complex research projecting

Low-dose retinoic acid enhances in vitro invasiveness of human oral squamous-cell-carcinoma cell lines

Retinoids inhibit the proliferation of several types of tumour cells, and are used for patients with several malignant tumours. In this study, we examined the effect of retinoic acids (RAs) on the invasive potentials of the oral squamous cell carcinoma (SCC) cells, BHY and HNt. BHY cells expressed all of retinoid nuclear receptors (RARα, β, γ, and RXRα) and cytoplasmic retinoic acid binding proteins (CRABP1 and CRABP2). HNt cells lacked the expression of RARβ, but expressed other nuclear receptors and CRABPs. All-trans retinoic acid (ATRA) and 13-cis retinoic acid (13-cisRA) (10−6and 10−7M) inhibited the growth of the cells, but low-dose ATRA and 13-cisRA (10−8M) marginally affected the growth of the cells. Surprisingly, low-dose RAs enhanced the activity of tissue-type plasminogen activator (tPA), and activated pro-matrix metalloproteinases (proMMP2 and proMMP9). Activation of proMMP2 and proMMP9 was inhibited by aprotinin, a serine-proteinase, tPA inhibitor. Furthermore, low-dose RAs enhanced the in vitro invasiveness of BHY cells. These results indicate that low-dose RAs enhances the in vitro invasiveness of oral SCC cells via an activation of proMMP2 and proMMP9 probably mediated by the induction of tPA. © 2001 Cancer Research Campaign http://www.bjcancer.co