Visualization of Nd3+-doped Laf3 Nanoparticles For Near Infrared Bioimaging via Upconversion Luminescence at Multiphoton Excitation Microscopyvisualization of Nd3+-doped Laf3 Nanoparticles For Near Infrared Bioimaging via Upconversion Luminescence at Multiphoton Excitation Microscopy

Recent developments in the field of biophotonics facilitate the raise of interest to inorganic nanoparticles (NPs) doped with Nd 3+ ions, because of their near-infrared (NIR) absorption. These NPs are interesting bioimaging probes for deep tissue visualization, while they can also act as local thermometers in biological tissues. Despite the good possibilities for visualization of NPs with Nd 3+ ions in NIR spectral range, difficulties arise when studying the cellular uptake of these NPs using commercially available fluorescence microscopy systems, since the selection of suitable luminescence detectors is limited. However, Nd 3+ ions are able to convert NIR radiation into visible light, showing upconversion properties. In this paper we found optimal parameters to excite upconversion luminescence of Nd 3+ :LaF 3 NPs in living cells and to compare the distribution of the NPs inside the cell culture of human macrophages THP-1 obtained by two methods. Firstly, by detecting the upconversion luminescence of the NPs in VIS under NIR multiphoton excitation using laser scanning confocal microscopy and secondly, using transmission electron microscopy

Laser biospectroscopy and 5-ALA fluorescence navigation as a helpful tool in the meningioma resection

5-aminolevulinic acid (5-ALA) is a natural precursor of protoporphyrin IX (PP IX), which possesses fluorescent properties and is more intensively accumulated in tumor cells than in normal tissue. Therefore, the use of 5-ALA in the surgical treatment of intracranial tumors, particularly gliomas, has gained popularity in the last years, whereas its use in other intracranial pathological entities including meningiomas has been reported occasionally. This study describes a series of 28 patients with intracranial meningiomas, who were administered 5-ALA for a better visualization of tumor boundaries. Twelve patients underwent also laser spectroscopic analysis in order to confirm the visual impression of tumor tissue visualization. Bone infiltration was readily demonstrated. In one case, the tumor recurrence could have been prevented by removal of a tumor remnant, which would possibly have been better recognized if spectroscopic analysis had been used. Fluorescent navigation (FN) is a useful method for maximizing the radicality of meningioma surgery, particularly if the tumor infiltrates the bone, the skull base, and/or the surrounding structures

A case of brain abscess mimicking cystic brain tumor and showing intraoperative 5-aminolevulinic acid fluorescence: case report .

Intraoperative fluorescence diagnostics is proved highly sensitive and specific for surgery of highly malignant brain gliomas, meningiomas and metastases. The greatest capability of accumulating 5-ALA was found in oligodendrogliomas, piloid astrocytomas and gliomas with cystic components. In some cases, making the differential diagnosis between a cerebral cystic tumor and a brain abscess can be difficult due to the similarity of clinical and neuroimaging data. A 37 year old male was hospitalized in a local neurological hospital. On admission, he presented with headaches, nausea, episodic vomiting, and weakness in the left extremities. In the last few months the patient suffered from recurrent episods of sinusitis. Contrast-enhanced brain CT scans revealed a right parietal lobe lesion of irregular shape (70x35 mm) with perilesional contrast enhancement. Contrast-enhanced MRI showed a mass lesion, which was hypointense on T1 with a marked contrast accumulation at the periphery. To the differential diagnosis between the suspected abscess and malignant tumor, we used the method of intraoperative metabolic navigation with 5-ALA. To relive the severe tension of the brain tissue, a ultrasound-guided cyst puncture was carried out. As a result, a thick yellow purulent discharge (~30 mL) was drained. Microbiological analysis of cyst contents revealed the presence of the pathogenic Streptococcus alactolyticus in the abscess’s content. The 5-ALA-induced fluorescence can be successfully used in neurosurgery for the differential diagnosis between a intracerebral tumor cyst and an abscess in the brain