Reinstating olfactory bulb-derived limbic gamma oscillations alleviates depression-like behavioral deficits in rodents

Although the etiology of major depressive disorder remains poorly understood, reduced gamma oscillations is an emerging biomarker. Olfactory bulbectomy, an established model of depression that reduces limbic gamma oscillations, suffers from non-specific effects of structural damage. Here, we show that transient functional suppression of olfactory bulb neurons or their piriform cortex efferents decreased gamma oscillation power in limbic areas and induced depression-like behaviors in rodents. Enhancing transmission of gamma oscillations from olfactory bulb to limbic structures by closed-loop electrical neuromodulation alleviated these behaviors. By contrast, silencing gamma transmission by anti-phase closed-loop stimulation strengthened depression-like behaviors in naive animals. These induced behaviors were neutralized by ketamine treatment that restored limbic gamma power. Taken together, our results reveal a causal link between limbic gamma oscillations and depression-like behaviors in rodents. Interfering with these endogenous rhythms can affect behaviors in rodent models of depression, suggesting that restoring gamma oscillations may alleviate depressive symptoms

Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)1.

In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Thus, it is important to formulate on a regular basis updated guidelines for monitoring autophagy in different organisms. Despite numerous reviews, there continues to be confusion regarding acceptable methods to evaluate autophagy, especially in multicellular eukaryotes. Here, we present a set of guidelines for investigators to select and interpret methods to examine autophagy and related processes, and for reviewers to provide realistic and reasonable critiques of reports that are focused on these processes. These guidelines are not meant to be a dogmatic set of rules, because the appropriateness of any assay largely depends on the question being asked and the system being used. Moreover, no individual assay is perfect for every situation, calling for the use of multiple techniques to properly monitor autophagy in each experimental setting. Finally, several core components of the autophagy machinery have been implicated in distinct autophagic processes (canonical and noncanonical autophagy), implying that genetic approaches to block autophagy should rely on targeting two or more autophagy-related genes that ideally participate in distinct steps of the pathway. Along similar lines, because multiple proteins involved in autophagy also regulate other cellular pathways including apoptosis, not all of them can be used as a specific marker for bona fide autophagic responses. Here, we critically discuss current methods of assessing autophagy and the information they can, or cannot, provide. Our ultimate goal is to encourage intellectual and technical innovation in the field

Edge effect on the power law distribution of granular avalanches

Many punctuated phenomena in nature are claimed [e.g., by the theory of self-organized criticality (SOC)] to be power-law distributed. In our experiments on a three-dimensional pile of long-grained rice, we find that by only changing the boundary condition of the system, we switch from such power-law-distributed avalanche sizes to quasiperiodic system-spanning avalanches. Conversely, by removing ledges the incidence of system-spanning avalanches is significantly reduced. This may offer a perspective on new avalanche prevention schemes. In addition, our findings may help to explain why the archetype of SOC, the sandpile, was found to have power-law-distributed avalanches in some experiments, while in other experiments quasiperiodic system-spanning avalanches were found. © 2007 The American Physical Society

Influence of the driving rate in a two-dimensional rice pile model

We study the influence of the driving rate in the two-dimensional Oslo rice pile model. We find that the usual power-law behavior of the avalanche size distribution still holds for small avalanches, independent of the driving rate. The signature of fast driving is, however, the increase of the incidence rate of large avalanches. For larger driving rates, this increase is more prominent and spreads to smaller avalanche sizes. As a result, the mass flow due to large avalanches is increased much more than would be expected from an increase in driving rate alone. Fast driving leads to a dramatic increase in devastating avalanches, just before the continuous flow regime is reached. © 2008 The American Physical Society