An Engagement Levels Framework To Foster Interactions Across SOTL Collaboratories

Regional SoTL collaboratories can provide a natural fit with political boundaries and funding opportunities; interconnections across regions can provide critical mass to sustain ongoing community interactions. It is unrealistic to expect these facilities to use a common technical platform or to adopt common social organizations, so a Framework for interconnection would be valuable. Our prototype Engagement Levels Framework includes four levels of faculty involvement, each with differing facilities to support research and knowledge mobilization: Cooperative Research Projects: Faculty who engage with a Teaching Research Collaboratory through specific short-term supported projects, as individuals and as teams, primarily focused on enhancing the learning experience and student success in their own courses and with some secondary focus on enhancing teaching practice for their colleagues at the institutional or provincial levels (and beyond). This secondary focus requires some effort to distinguish localized versus generalizable factors contributing to the success or limitations of an intervention in teaching. Practitioner/Researcher Core Communities: Faculty with an ongoing mutual engagement as a community, functioning both as an inquiry community to enhance the learning experience and student success in their own courses and as the core of a larger community of practitioner/researchers in research-led teaching, including developing and sustaining knowledge mobilization resources to enhance research-led teaching practices and knowledge by their faculty colleagues. Knowledge Exchange Networks: Faculty who participate periodically in knowledge mobilization for their own research-led teaching and become regular contributors to a knowledge mobilization network, motivated by a spirit of scholarly reciprocity and by explicit engagement initiatives led by the core Practitioner/Researcher community members described above. Collections of Research-Informed Resources for Teaching: To achieve a true ‘network effect’ in application of the knowledge and resources for research-led teaching, facilities must be provided to engage many more faculty in occasional access. These faculty seek to improve the learning experience and student success in their own courses, but do not regularly contribute from their own expertise to extend those resources. The key ideas underlying this framework include the following: • Modularity in the framework allows multiple platform options at each level • Embedding of research results into adaptable artifacts – curriculum plans, learning activity designs, open educational resources, etc. – promotes knowledge mobilization; • Early involvement of resource creators with colleagues promotes reusability and adaptability of such resources, and optimizes institutional investment in faculty innovation and scholarship; • Ongoing knowledge exchange is fostered by a focus on object-centered conversations to extend knowledge, adapt resources and share insights; • Collection management enables occasional users to find the resources they seek and to encounter related teaching knowledge along with those resources; • Explicit efforts by the collaboratory community will support enriched levels of engagement by colleagues, to insure a sustainable and dynamic community. Of course, such facilities can only enable and support widespread adaptation of research-informed resources and mobilization of the underlying knowledge about teaching and learning. We must in parallel work with partners in our regions and disciplines to provide stronger rationale and motivation for mobilizing SoTL as research-led teaching

Bactericidal activity of avian complement: a contribution to understand avian-host tropism of Lyme borreliae

© The Author(s) 2021. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.Complement has been considered as an important factor impacting the host-pathogen association of spirochetes belonging to the Borrelia burgdorferi sensu lato complex, and may play a role in the spirochete's ecology. Birds are known to be important hosts for ticks and in the maintenance of borreliae. Recent field surveys and laboratory transmission studies indicated that certain avian species act as reservoir hosts for different Borrelia species. Nevertheless, our current understanding of the molecular mechanisms determining host tropism of Borrelia is still in its fledgling stage. Concerning the role of complement in avian-host tropism, only a few bird species and Borrelia species have been analysed so far. Here, we performed in vitro serum bactericidal assays with serum samples collected from four bird species including the European robin Erithacus rubecula, the great tit Parus major, the Eurasian blackbird Turdus merula, and the racing pigeon Columba livia, as well as four Borrelia species (B. afzelii, B. garinii, B. valaisiana, and B. burgdorferi sensu stricto). From July to September 2019, juvenile wild birds were caught using mist nets in Portugal. Racing pigeons were sampled in a loft in October 2019. Independent of the bird species analysed, all Borrelia species displayed an intermediate serum-resistant or serum-resistant phenotype except for B. afzelii challenged with serum from blackbirds. This genospecies was efficiently killed by avian complement, suggesting that blackbirds served as dead-end hosts for B. afzelii. In summary, these findings suggest that complement contributes in the avian-spirochete-tick infection cycle and in Borrelia-host tropism.Open Access funding enabled and organized by Projekt DEAL. This study received financial support from Fundação para a Ciência e a Tecnologia by the strategic program of MARE (MARE—UID/MAR/04292/2020) and the transitory norm contract DL57/2016/CP1370/CT89 to ACN, and the Portuguese National Institute of Health (INSA).info:eu-repo/semantics/publishedVersio

Measuring the Pharmacodynamic Effects of a Novel Hsp90 Inhibitor on HER2/neu Expression in Mice Using 89Zr-DFO-Trastuzumab

The positron-emitting radionuclide (89)Zr (t(1/2) = 3.17 days) was used to prepare (89)Zr-radiolabeled trastuzumab for use as a radiotracer for characterizing HER2/neu-positive breast tumors. In addition, pharmacodynamic studies on HER2/neu expression levels in response to therapeutic doses of PU-H71 (a specific inhibitor of heat-shock protein 90 [Hsp90]) were conducted.Trastuzumab was functionalized with desferrioxamine B (DFO) and radiolabeled with [(89)Zr]Zr-oxalate at room temperature using modified literature methods. ImmunoPET and biodistribution experiments in female, athymic nu/nu mice bearing sub-cutaneous BT-474 (HER2/neu positive) and/or MDA-MB-468 (HER2/neu negative) tumor xenografts were conducted. The change in (89)Zr-DFO-trastuzumab tissue uptake in response to high- and low-specific-activity formulations and co-administration of PU-H71 was evaluated by biodistribution studies, Western blot analysis and immunoPET. (89)Zr-DFO-trastuzumab radiolabeling proceeded in high radiochemical yield and specific-activity 104.3+/-2.1 MBq/mg (2.82+/-0.05 mCi/mg of mAb). In vitro assays demonstrated >99% radiochemical purity with an immunoreactive fraction of 0.87+/-0.07. In vivo biodistribution experiments revealed high specific BT-474 uptake after 24, 48 and 72 h (64.68+/-13.06%ID/g; 71.71+/-10.35%ID/g and 85.18+/-11.10%ID/g, respectively) with retention of activity for over 120 h. Pre-treatment with PU-H71 was followed by biodistribution studies and immunoPET of (89)Zr-DFO-trastuzumab. Expression levels of HER2/neu were modulated during the first 24 and 48 h post-administration (29.75+/-4.43%ID/g and 41.42+/-3.64%ID/g, respectively). By 72 h radiotracer uptake (73.64+/-12.17%ID/g) and Western blot analysis demonstrated that HER2/neu expression recovered to baseline levels.The results indicate that (89)Zr-DFO-trastuzumab provides quantitative and highly-specific delineation of HER2/neu positive tumors, and has potential to be used to measure the efficacy of long-term treatment with Hsp90 inhibitors, like PU-H71, which display extended pharmacodynamic profiles

Learning Outcomes Assessment A Practitioner\u27s Handbook

Ontario’s colleges and universities have made strides in developing learning outcomes, yet effective assessment remains a challenge. Learning Outcomes Assessment A Practitioner\u27s Handbook is a step-by-step resource to help faculty, staff, academic leaders and educational developers design, review and assess program-level learning outcomes. The handbook explores the theory, principles, reasons for and methods behind developing program-level learning outcomes; emerging developments in assessment; and tips and techniques to build institutional culture, increase faculty involvement and examine curriculum-embedded assessment. It also includes definitions, examples, case studies and recommendations that can be tailored to specific institutional cultures.https://scholar.uwindsor.ca/ctlreports/1005/thumbnail.jp

Discutindo a deficiência com alunos do ensino fundamental e médio

A inclusão de alunos com deficiência em sala de ensino regular é um assunto controversa que remete à três grandes temas: a perspectiva de melhora para a criança atípica, a construção de uma sociedade inclusiva e consciente da diversidade, e a qualidade do ensino. Enquanto maioria dos estudos investigaram o ponto de vista dos profissionais da saúde e do ensino, o presente trabalho teve como foco principal alunos neurotípicos do ensino fundamental e médio. Através de aplicação de questionários e palestras interativas foi possível avaliar o entendimento da deficiência da parte desses alunos e as barreiras que travam o processo de inclusão escolar. Os resultados indicaram que 1) a complexidade do tema atrelada à constante revisão dos quadros de deficiência e a dificuldade de entendimento do vocabulário jurídico para o público leigo, 2) os preconceitos oriundos de séculos de práticas exclusivas, 3) a falta de divulgação das tecnologias assistivas e 4) a falta de convivência com indivíduos neurodiversos, são tantos fatores que dificultam a aceitação e o acolhimento das pessoas com deficiência. Campanhas de sensibilização e educativas são imprescindíveis para que todos possam entender as manifestações dos diferentes quadros de deficiência e saibam como incluir o outro

Discutindo a deficiência com alunos do ensino fundamental e médio

A inclusão de alunos com deficiência em sala de ensino regular é um assunto controversa que remete à três grandes temas: a perspectiva de melhora para a criança atípica, a construção de uma sociedade inclusiva e consciente da diversidade, e a qualidade do ensino. Enquanto maioria dos estudos investigaram o ponto de vista dos profissionais da saúde e do ensino, o presente trabalho teve como foco principal alunos neurotípicos do ensino fundamental e médio. Através de aplicação de questionários e palestras interativas foi possível avaliar o entendimento da deficiência da parte desses alunos e as barreiras que travam o processo de inclusão escolar. Os resultados indicaram que 1) a complexidade do tema atrelada à constante revisão dos quadros de deficiência e a dificuldade de entendimento do vocabulário jurídico para o público leigo, 2) os preconceitos oriundos de séculos de práticas exclusivas, 3) a falta de divulgação das tecnologias assistivas e 4) a falta de convivência com indivíduos neurodiversos, são tantos fatores que dificultam a aceitação e o acolhimento das pessoas com deficiência. Campanhas de sensibilização e educativas são imprescindíveis para que todos possam entender as manifestações dos diferentes quadros de deficiência e saibam como incluir o outro

Imprinting fidelity in mouse iPSCs depends on sex of donor cell and medium formulation

Funding Information: We would like to thank Sérgio de Almeida, Miguel Casanova and Inês Milagre for critical reading of the manuscript, and the members of the S.T.d.R.’s team for helpful discussions. We also thank Tânia Carvalho and Pedro Ruivo for their help in histological analysis; Judith Webster at Babraham Institute for LC-MS measurements; Bethan Hussey at Sanger Sequencing and Kristina Tabbada at Babraham Institute for assistance with high-throughput sequencing; and the Bioimaging unit as well as Andreia Santos, Rute Gonçalves and Mariana Fernandes of the Flow Cytometry Facility of Instituto de Medicina Molecular João Lobo Antunes for their services and assistance. Work in S.T.d.R.’s team was supported by Fundação para a Ciência e Tecnologia (FCT) Ministério da Ciência, Tecnologia e Ensino Superior (MCTES), Portugal [IC&DT projects PTDC/BEX-BCM/2612/2014 and PTDC/BIA-MOL/29320/2017 as well as projects UIDB/04565/2020 and UIDP/04565/2020 of the Research Unit Institute from Bioengineering and Biosciences – iBB and LA/P/0140/2020 of the Associate Laboratory Institute for Health and Bioeconomy – i4HB]; S.T.d.R. and A.-V.G. are supported by assistant research contracts from FCT/MCTES (CEECIND/01234/2017 and CEECIND/02085/2018, respectively); M.A and A.C.R. are supported, respectively, by SFRH/BD/151251/2021 and SFRH/BD/137099/2018 PhD fellowships from FCT/MCTES. J.V.G.L is supported by COVID/BD/152624/2022 from FCT/MCTES. MAE-M was supported by a BBSRC Discovery Fellowship (BB/T009713/1) and is now supported by a Snow Medical Fellowship. F.K. is supported by the Babraham Institute Strategic Core Funding and A.M. by BBSRC BBS/E/B/000C0421. B.B.J. work was funded by Fundação para a Ciência e Tecnologia (FCT), and FEDER, LISBOA-01-0145-FEDER-028534, project co-funded by FEDER, through POR Lisboa 2020—Programa Operacional Regional de Lisboa. T.K. is supported by Janko Jamnik Doctoral Scholarship from National Institute of Chemistry. Funding Information: We would like to thank Sérgio de Almeida, Miguel Casanova and Inês Milagre for critical reading of the manuscript, and the members of the S.T.d.R.’s team for helpful discussions. We also thank Tânia Carvalho and Pedro Ruivo for their help in histological analysis; Judith Webster at Babraham Institute for LC-MS measurements; Bethan Hussey at Sanger Sequencing and Kristina Tabbada at Babraham Institute for assistance with high-throughput sequencing; and the Bioimaging unit as well as Andreia Santos, Rute Gonçalves and Mariana Fernandes of the Flow Cytometry Facility of Instituto de Medicina Molecular João Lobo Antunes for their services and assistance. Work in S.T.d.R.’s team was supported by Fundação para a Ciência e Tecnologia (FCT) Ministério da Ciência, Tecnologia e Ensino Superior (MCTES), Portugal [IC&DT projects PTDC/BEX-BCM/2612/2014 and PTDC/BIA-MOL/29320/2017 as well as projects UIDB/04565/2020 and UIDP/04565/2020 of the Research Unit Institute from Bioengineering and Biosciences – iBB and LA/P/0140/2020 of the Associate Laboratory Institute for Health and Bioeconomy – i4HB]; S.T.d.R. and A.-V.G. are supported by assistant research contracts from FCT/MCTES (CEECIND/01234/2017 and CEECIND/02085/2018, respectively); M.A and A.C.R. are supported, respectively, by SFRH/BD/151251/2021 and SFRH/BD/137099/2018 PhD fellowships from FCT/MCTES. J.V.G.L is supported by COVID/BD/152624/2022 from FCT/MCTES. MAE-M was supported by a BBSRC Discovery Fellowship (BB/T009713/1) and is now supported by a Snow Medical Fellowship. F.K. is supported by the Babraham Institute Strategic Core Funding and A.M. by BBSRC BBS/E/B/000C0421. B.B.J. work was funded by Fundação para a Ciência e Tecnologia (FCT), and FEDER, LISBOA-01-0145-FEDER-028534, project co-funded by FEDER, through POR Lisboa 2020—Programa Operacional Regional de Lisboa. T.K. is supported by Janko Jamnik Doctoral Scholarship from National Institute of Chemistry. Publisher Copyright: © 2022, The Author(s).Reprogramming of somatic cells into induced Pluripotent Stem Cells (iPSCs) is a major leap towards personalised approaches to disease modelling and cell-replacement therapies. However, we still lack the ability to fully control the epigenetic status of iPSCs, which is a major hurdle for their downstream applications. Epigenetic fidelity can be tracked by genomic imprinting, a phenomenon dependent on DNA methylation, which is frequently perturbed in iPSCs by yet unknown reasons. To try to understand the causes underlying these defects, we conducted a thorough imprinting analysis using IMPLICON, a high-throughput method measuring DNA methylation levels, in multiple female and male murine iPSC lines generated under different experimental conditions. Our results show that imprinting defects are remarkably common in iPSCs, but their nature depends on the sex of donor cells and their response to culture conditions. Imprints in female iPSCs resist the initial genome-wide DNA demethylation wave during reprogramming, but ultimately cells accumulate hypomethylation defects irrespective of culture medium formulations. In contrast, imprinting defects on male iPSCs depends on the experimental conditions and arise during reprogramming, being mitigated by the addition of vitamin C (VitC). Our findings are fundamental to further optimise reprogramming strategies and generate iPSCs with a stable epigenome.publishersversionpublishe

Redução do Estresse do Estudante de Enfermagem: Um Estudo com Auriculoterapia

A auriculoterapia chinesa tem se mostrado eficaz na prevenção, controle e tratamento de várias enfermidades de ordem física, psicológicas e mentais, além de proporcionar uma melhor qualidade de vida. O objetivo desse trabalho foi avaliar a eficiência da auriculoterapia com sementes de mostarda na diminuição dos níveis de estresse dos estudantes de graduação e pós-graduação em enfermagem. Trata-se de um Estudo Clínico Randomizado, com uma amostra de 82 estudantes com score médio e alto conforme a Lista de Sintomas de Stress de Vasconcelos, onde foram divididos em 2 grupos: Controle (41) e Intervenção com auriculoterapia (41). Foram reavaliados após 8 sessões. Na análise encontrou-se diferença estatisticamente significativa entre os grupos de auriculoterapia e controle sem intervenção, segundo o teste t para amostras independentes (p<0,05). A redução dos níveis de estresse para o grupo auriculoterapia foi de 34% de redução dos níveis de estresse (grande decréscimo), correspondente ao índice de Cohen de 1,33 (muito grande efeito). Conclui-se que a auriculoterapia com sementes de mostarda foi eficaz em reduzir os níveis de estresse segundo a Lista de Sintomas de Stress de Vasconcellos

Efeitos da posição prona em pacientes com SARS-CoV-2 em uso da ventilação mecânica não-invasiva / Effects of prone position in SARS-CoV-2 patients on non-invasive mechanical ventilation

1 INTRODUÇÃOO posicionamento prono (PP) é considerado uma das estratégias de resgate de hipoxemia resistente mais efetivas para pacientes com síndrome do desconforto respiratório agudo (SDRA) de moderado a grave. Seus efeitos são pesquisados desde os anos 70, mostrando um incremento importante da oxigenação, principalmente por melhorar a relação ventilação-perfusão, reduzindo o espaço morto fisiológico. Por este motivo, a posição prona tem sido empregada nos pacientes com SDRA por coronavírus (GATTINONI et al, 2019).É estudado se os benefícios observados em pacientes em ventilação mecânica invasiva se repetem nos indivíduos com SDRA por coronavírus de leve a moderada, com PAO2/FiO2 >150 mmHg. 2 OBJETIVO Por meio de uma revisão integrativa, encontrar nas evidências os efeitos da PP na oxigenação e taxa de mortalidade em pacientes com infecção por coronavírus em ventilação mecânica não-invasiva (VNI). 3 MÉTODOConduzida a busca na MedLine, PubMed em Novembro/2020, utilizando as palavras-chave “Prone Position” e  “Coronavirus Infections” pertencentes ao vocabulário do MesH. O operador Booleano foi “AND”. A estratégia de busca aplicada foi: (Prone Position) AND (Coronavirus Infections). Os materiais encontrados foram triados primeiro por título, depois por resumo e então lido integralmente para a extração dos dados.Critérios de elegibilidade: Amostra composta por pacientes com infecção por coronavírus em VNI; Ter PP como intervenção; Ter como desfechos oxigenação e/ou taxa  de mortalidade; Artigos publicados após 2019. 4 RESULTADOSNa busca realizada na PubMed resultou em 108 artigos, após leitura de título e resumo, foram excluídos 99, restando 9 trabalhos para a análise de texto completo. Desses, 4 foram excluídos, 2 por se tratarem de carta ao editor, 1 por ser realizado em UTI e 1 por não atender aos desfechos oxigenação e/ou taxa de mortalidade.McNicholas et al 2020,  conclui em sua revisão que a posição prona não traz melhora efetiva na oxigenação. Já COPPO et al 2020 realizaram um estudo de coorte prospectivo, com pacientes diagnosticados com pneumonia por coronavírus, com idade entre 18–75 anos, em oxigenoterapia por VNI. Manteve-se a posição prona por pelo menos 3 horas, gasometrias foram coletados 10 min em prono e 1 hora depois de retornar para supino. Os autores encontraram um aumento considerável na oxigenação na manutenção do decúbito ventral, esse incremento se manteve após o retorno a supino, em metade dos pacientes.TABOADA et al 2020, testaram os efeitos do posicionamento prono por uma hora, em pacientes internados na enfermaria. Foram monitoradas a saturação periférica e oxigenação na gasometria. Metade dos indivíduos que pronaram aumentaram 2% na saturação, e 62% dos pacientes obtiveram aumento significativo no índice de oxigenação durante e após a pronação. Os autores concluem que a posição prona é segura, facilmente aplicada e eficaz para aumentar a oxigenação arterial e saturação em pacientes na enfermaria. Recomendam, ainda, a adoção da posição prona pelo menos 30 min 3x ao dia de acordo com a tolerância do paciente.WINEARLS et al 2020 realizaram um estudo retrospectivo, e observaram 24 pacientes em ventilação não invasiva (CPAP) foram pronados ou semipronados, de acordo com a tolerância, por em média 8h diárias. Dos 24 pacientes, 2 não suportaram por dor ou piora na oxigenação. Ambos os grupos obtiveram melhora importante na oxigenação, comparando pacientes que se mantiveram em supino, mas não observou-se diferença significativa entre os dois grupos. Já no estudo de coorte prospectivo de HALLIFAX, ROB, 2020 os resultados mostraram uma associação com uma menor taxa de mortalidade no grupo de prono, quando comparado com o semiprono. 5 CONCLUSÃOA posição prona, ou semiprona, é segura e eficaz para a melhora de oxigenação arterial em pacientes com pneumonia por coronavírus submetidos à VNI. Ainda não há evidências suficientes para relacionar a posição prona reduz a mortalidade