Time-resolved deuterium–deuterium fusion born 1 MeV triton confinement study in EAST deuterium plasma

ORCID　0000-0003-4555-1837A time-resolved deuterium–deuterium (D–D) fusion-born triton confinement study, aimed at understanding alpha particle confinement ability, was performed in Experimental Advanced Superconducting Tokamak (EAST) deuterium plasmas for the first time. A scintillating fiber detector was developed for measuring the secondary deuterium–tritium (D–T) neutrons, which provide evidence of triton slowdown, in EAST. The D–D fusion-born triton confinement experiment was performed by measuring secondary D–T neutrons in D-beam-heated D plasma with a plasma current of 400 kA. The secondary D–T neutron signal and its time evolution were obtained using pulse height discrimination analysis. The D–T neutron rate was calculated using the classical energetic ion confinement model to clarify the D–T neutron measurements. The secondary D–T neutron emission rate obtained from the numerical simulation closely agrees with the experimentally obtained results when considering the prompt loss of the tritons.journal articl

CRISPR/Cas9 Screen Uncovers Functional Translation of Cryptic lncRNA-Encoded Open Reading Frames in Human Cancer

Emerging evidence suggests that cryptic translation within long noncoding RNAs (lncRNAs) may produce novel proteins with important developmental/physiological functions. However, the role of this cryptic translation in complex diseases (e.g., cancer) remains elusive. Here, we applied an integrative strategy combining ribosome profiling and CRISPR/Cas9 screening with large-scale analysis of molecular/clinical data for breast cancer (BC) and identified estrogen receptor α-positive (ER+) BC dependency on the cryptic ORFs encoded by lncRNA genes that were upregulated in luminal tumors. We confirmed the in vivo tumor-promoting function of an unannotated protein, GATA3-interacting cryptic protein (GT3-INCP) encoded by LINC00992, the expression of which was associated with poor prognosis in luminal tumors. GTE-INCP was upregulated by estrogen/ER and regulated estrogen-dependent cell growth. Mechanistically, GT3-INCP interacted with GATA3, a master transcription factor key to mammary gland development/BC cell proliferation, and coregulated a gene expression program that involved many BC susceptibility/risk genes and impacted estrogen response/cell proliferation. GT3-INCP/GATA3 bound to common cis regulatory elements and upregulated the expression of the tumor-promoting and estrogen-regulated BC susceptibility/risk genes MYB and PDZK1. Our study indicates that cryptic lncRNA-encoded proteins can be an important integrated component of the master transcriptional regulatory network driving aberrant transcription in cancer, and suggests that the hidden lncRNA-encoded proteome might be a new space for therapeutic target discovery

Bone Metastasis Initiation Is Coupled with Bone Remodeling through Osteogenic Differentiation of NG2+ Cells

The bone microenvironment is dynamic and undergoes remodeling in normal and pathologic conditions. Whether such remodeling affects disseminated tumor cells (DTC) and bone metastasis remains poorly understood. Here, we demonstrated that pathologic fractures increase metastatic colonization around the injury. NG2+ cells are a common participant in bone metastasis initiation and bone remodeling in both homeostatic and fractured conditions. NG2+ bone mesenchymal stem/stromal cells (BMSC) often colocalize with DTCs in the perivascular niche. Both DTCs and NG2+ BMSCs are recruited to remodeling sites. Ablation of NG2+ lineage impaired bone remodeling and concurrently diminished metastatic colonization. In cocultures, NG2+ BMSCs, especially when undergoing osteodifferentiation, enhanced cancer cell proliferation and migration. Knockout of N-cadherin in NG2+ cells abolished these effects in vitro and phenocopied NG2+ lineage depletion in vivo. These findings uncover dual roles of NG2+ cells in metastasis and remodeling and indicate that osteodifferentiation of BMSCs promotes metastasis initiation via N-cadherin-mediated cell-cell interaction. SIGNIFICANCE: The bone colonization of cancer cells occurs in an environment that undergoes constant remodeling. Our study provides mechanistic insights into how bone homeostasis and pathologic repair lead to the outgrowth of disseminated cancer cells, thereby opening new directions for further etiologic and epidemiologic studies of tumor recurrences. This article is highlighted in the In This Issue feature, p. 247

Enhancing the correlation between azimuth and Doppler frequency for speed estimation of nearby tangential targets

Abstract With the development of intelligent transportation systems, traffic supervision radar with wide coverage plays a crucial role in traffic management and vehicle-road coordination. The correlation between Doppler frequency and azimuth has been widely validated in wide coverage traffic supervision radar for high-precision velocity measurement. However, angular glint and noise of the nearby targets lead to a decrease in correlation between the azimuth and Doppler frequency, which negatively impacts the accuracy of velocity estimation. Currently, adopting separate filtering strategies for target azimuth and Doppler frequency has limited performance in enhancing correlation. This paper presents a joint observation model for azimuth and Doppler frequency to achieve the extraction of interrelated components from subspaces, which improves the accuracy of velocity measurement. The effectiveness of this approach is validated using data obtained from X-band and Ku-band sensors

A Multi-Parameter Calibration Method Based on the Newton Method and the Genetic Algorithm in Airborne Array Synthetic Aperture Radar

Airborne array synthetic aperture radar (SAR) can achieve three-dimensional (3D) imaging of the observed scene in a single flight. Nevertheless, the imaging process of airborne array SAR is subject to various parameter errors due to unstable factors. Such errors degrade the quality of 3D imaging, particularly for the elevation imaging results, which necessitates the employment of super-resolution algorithms. The most significant error parameters include the amplitude and phase imbalances between multiple channels, as well as the phase-center positions of each channel. Owing to the coupled nature of these parameter errors, the calibration accuracy for each parameter independently is relatively sub-par, while super-resolution algorithms have strict demands for parameter precision. Addressing these challenges, this article proposes a multi-parameter calibration method for airborne array SAR based on the Newton method and the genetic algorithm. Initially, a least squares model for multi-parameter calibration is established, followed by leveraging the global optimization characteristics of genetic algorithms and the rapid convergence property of the Newton method. The genetic algorithm is utilized to locate a sub-optimal solution in proximity to the optimal one, subsequently converging swiftly to the optimal solution via the Newton method, which incorporates second-order information. This approach averts the pitfalls of local convergence due to large initial value errors, thereby enhancing the algorithm’s robustness. The proposed method effectively enhances the precision of multi-parameter calibration, which is of significant importance in ensuring the quality of 3D imaging of airborne array SAR