68 research outputs found

    A role for CITED2, a CBP/p300 interacting protein, in colon cancer cell invasion

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    Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/116311/1/feb2s0014579307012264.pd

    Transcription factor ZBP-89 is required for STAT1 constitutive expression

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    IFNγ is a pro-inflammatory cytokine that potentiates p53-independent apoptosis in a variety of cell types. STAT1 is the primary mediator of IFNγ action. ZBP-89 is a transcription factor that binds to the G/C-rich elements and mediates p53-independent apoptosis. In this study, site-directed mutagenesis revealed that a G-rich element from +171 to +179 within the first intron of the STAT1 gene is critical for optimal STAT1 promoter activity. Electrophoretic mobility shift assays and promoter analysis revealed that ZBP-89 binds directly to this STAT1 G-rich element along with Sp1 and Sp3. Reduction of ZBP-89 with siRNA attenuated both basal and IFNγ-induced STAT1 expression and subsequently diminished the activation of apoptotic markers, e.g. caspase-3 and PARP. Taken together, we conclude that ZBP-89 is required for constitutive STAT1 expression and in this way contributes to the ability of cells to be activated by IFNγ

    Piperidine-2,6-dione derivatives as cereblon-binding agents and their preparation

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    http://deepblue.lib.umich.edu/bitstream/2027.42/191180/2/WO 2023-183607 Al.pdfDescription of WO 2023-183607 Al.pdf : Published versio
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