Is open lateral anal sphincterotomy really a safe and satisfactory treatment option for refractory chronic anal fissures? - a prospective study

Background: Chronic anal fissure is a familiar entity in surgical outdoor departments of hospitals in our valley. The muslim women are usually reluctant to expose their anal canal related pathologies to male surgeons, letting anal fissures to reach the chronic stage. Under this background, the present study was conducted to look for the feasibility of lateral anal sphincterotomy in the management of chronic anal fissure in our patients.Methods: This prospective study was carried out over a period of 3 years in the unit 2nd of department of surgery at SMHS (Shri Maharaja Harisingh) hospital, an associated hospital of Government Medical College Srinagar. During this period, 59 patients presented to the outpatient department with typical chronic anal fissures and were included in this study.Results: Fifty-nine patients, diagnosed on clinical evaluation as chronic anal fissure were included in this study. The age varied from 19 to 58 years with mean age of 36.38±7.14 (SD= 7.14) years. There was a female predominance, with a female to male ratio of 2.1:1. Fifty-two (88.1%) patients had posterior midline fissure and 7 (11.8%) patients had an anterior anal fissure. Thirty-one patients were not satisfied with the conservative treatment and insisted for surgical management. All 31 patients were managed by open lateral anal sphincterotomy.Conclusions: Lateral anal sphincterotomy (LAS) is a safe and effective method of management for chronic refractory anal fissures. The complications are minimal and negligible

Evidence of apoptosis in some cell types due to pentachlorophenol (PCP) in Heteropneustes fossilis

AbstractThe study aimed to clarify the role of apoptosis in pentachlorophenol (PCP) induced testicular, ovarian and renal cell genotoxicity of Heteropneustes fossilis. It was further intended to find the target germ cell type and assess the cellular and nuclear damage. Treatment of PCP was used for multiduration on the germinal tissues and they were processed to detect structural changes by light and electron microscopic evaluation and kidney cells for subsequent detection of DNA fragmentation by agarose gel electrophoresis. Findings suggest functional and morphological changes in the tissues are due to apoptosis, as evidenced by some biochemical and cytological signs. Histological observation on germinal epithelium reveals cell suicidal symptoms such as vacuolization, liquefied regions in the cytoplasm of oocytes, margination of nuclei, clumping of chromatin, and compaction of cytoplasmic organelle. Biochemical manifestation concurrent to this, is; cleavage of kidney cell DNA into low molecular weight fragments confirming apoptosis. Subsequently, it is further cleaved into nucleosome size fragments or its multiples. Ultra-structural histopathology and DNA studies conclusively lead to the PCP induced apoptosis in the exposed cell types. Results further support the usefulness of this assay in the related studies and its feasibility in generating a base line data

Investigating the Role of TP53 in Peripheral T-Cell Lymphoma-GATA3 Subtype

Introduction: Non-Hodgkin Lymphoma (NHL) accounts for 4.1% of all cancers in the United States. Peripheral T-Cell Lymphoma (PTCL) consists of ~10-15% of all NHL in the Western world. 30-50% of these PTCLs are not classifiable/diagnosed and are instead designated as PTCL-Not Otherwise Specified (PTCL-NOS). The two major molecular subgroups within PTCL-NOS are PTCL-TBX21 and PTCL-GATA3, determined by their distinct T-helper (TH) transcriptional programs. GATA3 and TBX21 are the master-transcriptional regulators of TH2- and TH1-cell differentiation, respectively. The overall survival analysis of PTCL-NOS cases illustrates the clinical outcome of PTCL-GATA3 cases are significantly lower than PTCL-TBX21 cases over a broad timeframe. Thus, the need for understanding the underlying mechanism and finding therapeutic targets is at the utmost importance. Background: TP53 mutations and/or TP53 loss deletions are frequent in PTCL-GATA3 cases, compared to PTCL-TBX21. TP53 is a protein that is essential in cycle regulation but also acts as a tumor suppressor. It stops cells from dividing if they have mutated or damaged DNA. Due to the high mutation rates observed in this subtype, we believe TP53 could play a major role in this mechanism. Therefore, it was important to focus on the TP53-GATA3 interaction at the genomic level. Prior studies using chromatin immunoprecipitation (ChIP)-qPCR on the intron 3 full GATA3 region suggested there was more TP53 binding in this intron region compared to other regions. Therefore, we designed a research strategy to determine the specific binding regions of TP53-GATA3 interaction and the function of the TP53 binding.https://digitalcommons.unmc.edu/surp2023/1003/thumbnail.jp

Role of Diffusion-Weighted and Chemical Shift Magnetic Resonance Imaging in Differentiation of Benign and Malignant Spinal Fractures

Atraumatic spinal compression fractures are common clinical problem. Differentiating benign osteoporotic fractures from pathological fractures due to malignant/metastatic lesions affects the management and prognoses in patients with known extraspinal malignancy.    The objective of the research was to assess the role of conventional magnetic resonance imaging sequences with diffusion-weighted imaging and chemical-shift imaging in differentiating benign and malignant acute spinal compression fractures. Materials and Methods. The study included 40 patients with acute spinal compression fractures. Patients were evaluated using magnetic resonance imaging with diffusion-weighted imaging and chemical-shift imaging to differentiate benign etiology from malignant one. The results obtained were compared with histopathological follow-up for 6 months for definite clinical diagnoses. Results. No significant difference was noted in signal characteristics of benign and malignant fractures on T1, T2 and short-tau inversion recovery. However, posterior element involvement, soft tissue component and post-contrast enhancement were seen more frequently in malignant fractures (p<0.05). On diffusion-weighted images, 77.8% of malignant fractures were hyperintense and 59.1% of benign fractures were hypointense (p<0.05). The mean apparent diffusion coefficient value was 0.81 ± 0.19 for malignant and 1.24 ± 0.24 for benign fractures (p<0.5). The mean signal intensity ratio for malignant fractures was 0.91 ± 0.125, whereas the signal intensity ratio for benign fractures was 0.64 ± 0.096 (p<0.001). Conclusions. Signal characteristics on T1, T2 and short-tau inversion recovery sequences do not differentiate benign from malignant fractures; however, posterior element involvement, soft tissue and post contrast enhancement help in differentiating the etiology. Diffusion-weighted imaging and apparent diffusion coefficient values, as well as using chemical shift imaging further improve the diagnostic accuracy of magnetic resonance imaging

Numerical simulations of heat generation, thermal radiation and thermal transport in water-based nanoparticles: OHAM study

Abstract This study investigates the 3D flow properties and heat transfer of copper, titanium/ water nanofluids across a bidirectional surface under the impact of MHD. The thermophysical features of nanofluid are employed using the Tiwari and Das model. Boundary layer theory has simplified the resulting physical principles. By using the proper transformations, the complicated sets of connected PDEs have evolved into ODEs. Equations that have been modify by using OHAM. For various dimensionless component ranges between $$2\le M\le 10$$ 2 ≤ M ≤ 10 . $$0\le a\le 3$$ 0 ≤ a ≤ 3 , $$0.05\le \varphi \le 0.08$$ 0.05 ≤ φ ≤ 0.08 , $$3\le \mathrm{Pr}\le 8$$ 3 ≤ Pr ≤ 8 , $$0\le Rd\le 6$$ 0 ≤ R d ≤ 6 , and $$2\le \lambda \le 4$$ 2 ≤ λ ≤ 4 the results are investigated computationally and graphically. It is observed that fluid parameters improve; they react differently from temperature and velocity profile. Additionally, thermal profiles decrease in comparison to greater Eckert and Prandtl numbers

Quadratic-Phase Wave-Packet Transform in L2(R)

Wavelet transform is a powerful tool for analysing the problems arising in harmonic analysis, signal and image processing, sampling, filtering, and so on. However, they seem to be inadequate for representing those signals whose energy is not well concentrated in the frequency domain. In pursuit of representations of such signals, we propose a novel time-frequency transform coined as quadratic-phase wave packet transform in L2(R). The proposed transform is aimed at rectifying the conventional wavelet transform by employing a quadratic-phase Fourier transform with extra degrees of freedom. Besides the formulation of all the fundamental results, including the orthogonality relation, reconstruction formula and the characterization of range, we also derive a direct relationship between the well-known Wigner-Ville distribution and the proposed transform. In addition, we study the quadratic-phase wave-packet transform in the framework of almost periodic functions. Finally, we extend the scope of the present work by investigating the composition of quadratic-phase wave packet transforms

Quadratic-Phase Wave-Packet Transform in <i><b>L</b></i>²(<inline-formula><math display="inline"><semantics><mrow><mi mathvariant="double-struck">R</mi></mrow></semantics></math></inline-formula>)

Wavelet transform is a powerful tool for analysing the problems arising in harmonic analysis, signal and image processing, sampling, filtering, and so on. However, they seem to be inadequate for representing those signals whose energy is not well concentrated in the frequency domain. In pursuit of representations of such signals, we propose a novel time-frequency transform coined as quadratic-phase wave packet transform in L2(R). The proposed transform is aimed at rectifying the conventional wavelet transform by employing a quadratic-phase Fourier transform with extra degrees of freedom. Besides the formulation of all the fundamental results, including the orthogonality relation, reconstruction formula and the characterization of range, we also derive a direct relationship between the well-known Wigner-Ville distribution and the proposed transform. In addition, we study the quadratic-phase wave-packet transform in the framework of almost periodic functions. Finally, we extend the scope of the present work by investigating the composition of quadratic-phase wave packet transforms

Discrete Quadratic-Phase Fourier Transform: Theory and Convolution Structures

The discrete Fourier transform is considered as one of the most powerful tools in digital signal processing, which enable us to find the spectrum of finite-duration signals. In this article, we introduce the notion of discrete quadratic-phase Fourier transform, which encompasses a wider class of discrete Fourier transforms, including classical discrete Fourier transform, discrete fractional Fourier transform, discrete linear canonical transform, discrete Fresnal transform, and so on. To begin with, we examine the fundamental aspects of the discrete quadratic-phase Fourier transform, including the formulation of Parseval&rsquo;s and reconstruction formulae. To extend the scope of the present study, we establish weighted and non-weighted convolution and correlation structures associated with the discrete quadratic-phase Fourier transform