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Not AvailableIn agricultural or animal experiment, when a series of tasks are assigned to the experimental units under different environmental conditions, it is often a tedious job to alter the conditions at the end of each task period. Hence, each unit is put to perform all the assigned tasks under one set of conditions during one session (environment) and then the conditions are altered for the next session for the next series of tasks. The setting is such that there is a gap between each main session and hence it is assumed that no carry over effect transfers from a main session to another. But carry over effects are assumed to be present within main sessions, between sub-sessions. Designs involving sequences of treatment combinations of two factors, one nested within the other, are suitable for such situations. A general method of construction of such designs has been obtained here. The method of construction has been illustrated b to series of designs wherein the second series is a rearrangement of the first one and used for comparative study. It was observed that the precision of estimation of direct and carry over affects is more with he design having more number of levels of the nested factor.Not Availabl

Expression of Intrahepatic Inducible Nitric oxide Synthetase mRNA Correlates With Production of Nitric Oxide During Intraportal Isogeneic and Allogeneic Rat Islet Transplantation

Intrahepatic NO production is related to the islet mass transplanted. Nitric oxide production is higher in recipients of allogeneic rather than syngeneic islets. In addition, in allogeneic recipients a possible second peak of NO production was observed at 120 hours corresponding to the time of cellular rejection of the islet grafts (P = .22 vs 96 hours). Finally, the time to rejection of Wistar rat donor islets transplanted into Lewis rat diabetic recipients treated with NMA was not affected. However, inhibiting NO production in the minimal islet transplant model decreased the time to islet function, it does not affect the time to clinical rejection in recipients of a high number of allogeneic islet, which functions immediately. High-level NO has been shown to inhibit T-cell activation in vitro, and thus decreasing the levels by administrating NMA may accentuate the rejection response, canceling out the beneficial effect that might otherwise have occurred on islet function. Further experiments are required to clarify these issues