The prevalence of BANA‐hydrolyzing periodontopathic bacteria in smokers

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/99012/1/j.1600-051X.1999.tb02526.x.pd

Bacterial Profiles of Subgingival Plaques in Periodontitis

Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/142259/1/jper0447.pd

Factors which are associated with dental decay in the older individual

Objectives: To improve reliability of salivary bacterial cultures as a surrogate for plaque levels of cariogenic bacterial species by reporting the salivary CFUs of these organisms as a function of the number of teeth. Design: Cross-sectional collection of data in a convenience sample of adults over 60 years of age. Setting: Hospital Dental clinic, University bacteriology laboratory. Subjects: 523 older dentate subjects, average age 70, including 412 subjects who were in an independent living status and 111 in a dependent-living situation. Main outcome measures : Subjects were examined for decay and the presence of salivary factors including the levels of S. mutans , lactobacilli, yeast and other bacteria. The salivary levels of the bacteria were adjusted for the number of teeth in the mouth, and the resultant values were entered into multivariable logistic regression models along with clinical and other salivary parameters. Results: Mutans streptococci levels reported as CFUs/ml saliva per tooth were significantly associated with coronal surface decay, and lactobacilli, reported in a similar way, were significantly associated with root surface decay. Salivary levels of yeasts, which had previously been associated with decay in this population, were no longer significant using this construct. Conclusions : This construct of reporting salivary bacteriological data as a function of tooth number and per ml saliva could improve the reliability of bacteriological data obtained in epidemiological studies investigating the role of bacteria in dental decay in the elderly.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/72626/1/j.1741-2358.1999.00037.x.pd

Microcapsules on Streptococcus mutans Serotypes by Electron Microscopy

Extracellular microcapsules have been demonstrated on cells of most serotypes of Streptococcus mutans by electron microscopy, using bacterial strains of the various serotypes and peroxidase labeled or unlabeled immune serum. A correlation was noted between the amount of capsular substance on the strains of S mutans examined and degree of antigenicity as expressed by the indirect fluorescent antibody (FA) title. A serotype d strain was shown to lose both antigenicity as determined by the FA reaction and capsular material as seen by electron microscopy with repeated in vitro passage. When 10% unheated rabbit serum was added to the medium, antigenicity and capsular material were restored.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/68107/2/10.1177_00220345770560021101.pd

Metronidazole in Periodontitis: I. Clinical and Bacteriological Results after 15 to 30 Weeks

Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/141752/1/jper0325.pd

Diagnostic potential of chromogenic substrates for rapid detection of bacterial enzymatic activity in health and disease associated periodontal plaques

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/65525/1/j.1600-0765.1984.tb01327.x.pd

Xerostomia, Xerogenic Medications and Food Avoidances in Selected Geriatric Groups

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/111175/1/j.1532-5415.1995.tb05815.x.pd

Identification of Streptococcus mutans serotypes in dental plaque by fluorescent antibody techniques

The presence of Streptococcus mutans in dental plaque may be related to human dental caries. The cultural identification of this organism in plaque is not likely to be used routinely in a clinical or epidemiological situation. In an effort to develop a simpler diagnostic means of identifying Strep. mutans in plaque, antisera were prepared for the 5 known serotypes of Strep. mutans. These immune sera were conjugated with fluorescein isothiocyanate, fractionated to obtain the gamma globulin and titrated against their homologous antigens to obtain working titres. These antisera were compared with cultural methods for their ability to detect Strep. mutans in plaque samples. Nonspecific fluorescent antibody reactions and weak cross-reactions were essentially eliminated by applying eriochrome black as a counterstain, after plaque and conjugated antiserum had been incubated. The results suggest that the fluorescent antibody is more sensitive than cultural methods in the detection of Strep. mutans in dental plaque.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/33874/1/0000135.pd

The effects of periodontal therapy on serum antibody (IgG) levels to plaque microorganisms *

The influence of periodontal therapy on serum antibody titers to selected periodontal disease-associated microorganisms was assessed in 23 patients having chronic inflammatory periodontal disease (CIPD), The immunoglobulin G (IgG) titers were dÉtÉrmined by the micro ELISA technique in serum samples obtained prior to treatment; following a hygienic phase which included scaling, root planing, and oral hygiene instruction; following surgical treatment; and one year and two years following hygienic phase (maintenance phase). Considerable individual variability existed in the magnitude of immune response to specific bacterial preparations. Significant reductions in the mean antibody titers were seen to A. viscosus. S. sanguis. F. nucleatum, S, spuligena, B. gingivalis. B. interme-dius. B. melaninogeniem, T. vincentii , and T denticola by the end of the second year of maintenance. There was no consistent response to Capnucytophaga. When individual patient responses were examined. 6 of the 23 were found to have elevated titers to at least one of the microorganisms in the interval between pretreatment and the end of the hygienic phase; however, in all but one case, the titers at the end of the second year of maintenance were below pretreatment levels. Antibody levels to bacteria such as S. sanguis were modified during therapy. This would indicate that immune responses to microbes not generally considered to be “periodontal pathogens” may be modified by adjuvant activity associated with subgingival plaque or changes in the environment of the sulcus and that subsequent changes in titer do not necessarily reflect a role of that microorganism in the disease process.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/75110/1/j.1600-051X.1988.tb02127.x.pd

Efficiency of split-mouth designs

. The purpose of this paper is (1) to investigate the similarity of the amount, distribution, and, severity of periodontal disease of the within-patient experimental units, (2) to estimate the relative efficiencies of split-mouth designs when compared to whole-mouth designs, and (3) to discuss how stratification on initial pocket depth can result in large differences in the power of the test-statistics in the different disease categories. Periodontal disease characteristics are not always homogeneously distributed over the within-patient experimental units and this heterogeneity can reduce the efficiency of split-mouth designs. In particular, if analyses are stratified on initial pocket depth, sites with an initial probing depth deeper than 6 mm may be small in number and asymmetrically distributed when compared to sites with an initial probing depth less than 6 mm. This may result in large differences of the power of the test statistics among the different disease categories and should lead to a careful interpretation of the statistical significance tests. When disease characteristics are symmetrically distributed over the within-patient experimental units and a sufficient number of sites is present per experimental unit, the split-mouth design can provide moderate to large gains in relative efficiency. In the absence of a symmetric disease distribution, wholemouth clinical trials may be preferable.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/75701/1/j.1600-051X.1990.tb01060.x.pd