Providing library and information support for education

The key achievements of V.O. Sukhomlynskyi State Scientific and Pedagogical Library of Ukraine at the NAES of Ukraine are characterised in the context ofproviding library and information support for educational process. The strategies of the library regarding the improvement ofproviding library and information support for innovative processes in the educational sphere of Ukraine under conditions of European integration are determined.Основними здобутками Державної науково-педагогічної бібліотеки України ім. В. О. Сухомлинського НАПН України є забезпечення інформаційно-бібліотечної підтримки навчального процесу. У статті визначено стратегії бібліотеки щодо вдосконалення інформаційно-бібліотечної підтримки інноваційних процесів в освітній сфері України в умовах Європейської інтеграції

Національна доповідь про стан і перспективи розвитку освіти в Україні: монографія (До 30-річчя незалежності України)

The publication provides a comprehensive analysis of the state and development of national education over the 30 years of Ukraine’s independence, identifies current problems in education, ascertains the causes of their emergence, offers scientifically reasoned ways to modernise domestic education in the context of globalisation, European integration, innovative development, and national self-identification. Designed for legislators, state officials, education institutions leaders, teaching and academic staff, the general public, all those who seek to increase the competitiveness of Ukrainian education in the context of civilisation changes.У виданні здійснено всебічний аналіз стану і розвитку національної освіти за 30-річний період незалежності України, визначено актуальні проблеми освітньої сфери, виявлено причини їх виникнення, запропоновано науково обґрунтовані шляхи модернізації вітчизняної освіти в умовах глобалізації, європейської інтеграції, інноваційного розвитку та національної самоідентифікації. Розраховано на законодавців, державних управлінців, керівників закладів освіти, педагогічних і науково-педагогічних працівників, широку громадськість, усіх, хто прагне підвищення конкурентоспроможності української освіти в контексті цивілізаційних змін

The contribution of σ70 and σN factors to expression of class II pilE in Neisseria meningitidis

Neisseria meningitidis expresses multi-component organelles called Type four pili (Tfp), which are key virulence factors required for attachment to human cells during carriage and disease. Pilin (PilE) is the main component of Tfp and N. meningitidis isolates have either a class I pilE locus and express pilins that undergo antigenic variation, or a class II pilE locus and express invariant pilins. The transcriptional regulation of class I pilE has been studied in both N. meningitidis and Neisseria gonorrhoeae, while the control of expression of class II pilE has been elucidated in the non-pathogenic Neisseria elongata. However, the factors that govern the regulation of the class II pilE gene in N. meningitidis are not known. In this work, we have bioinformatically and experimentally identified the class II pilE promoter. We confirmed the presence of conserved σ70 and σN-dependent promoters upstream of pilE in a collection of meningococcal genomes and demonstrate that class II pilE expression initiates from the σ70 family-dependent promoter. By deletion or overexpression of sigma factors, we show that σN, σH and σE do not affect class II pilin expression. These findings are consistent with a role of the housekeeping σD in expression of this important component of Tfp. Taken together our data indicate that the σ-dependent network responsible for the expression of class II pilE has been selected to maintain pilE expression, consistent with the essential roles of Tfp in colonisation and pathogenesis.</p

The contribution of σ70 and σN factors to expression of class II pilE in Neisseria meningitidis

Neisseria meningitidis expresses multi-component organelles called Type four pili (Tfp), which are key virulence factors required for attachment to human cells during carriage and disease. Pilin (PilE) is the main component of Tfp and N. meningitidis isolates have either a class I pilE locus and express pilins that undergo antigenic variation, or a class II pilE locus and express invariant pilins. The transcriptional regulation of class I pilE has been studied in both N. meningitidis and Neisseria gonorrhoeae, while the control of expression of class II pilE has been elucidated in the non-pathogenic Neisseria elongata. However, the factors that govern the regulation of the class II pilE gene in N. meningitidis are not known. In this work, we have bioinformatically and experimentally identified the class II pilE promoter. We confirmed the presence of conserved σ70 and σN-dependent promoters upstream of pilE in a collection of meningococcal genomes and demonstrate that class II pilE expression initiates from the σ70 family-dependent promoter. By deletion or overexpression of sigma factors, we show that σN, σH and σE do not affect class II pilin expression. These findings are consistent with a role of the housekeeping σD in expression of this important component of Tfp. Taken together our data indicate that the σ-dependent network responsible for the expression of class II pilE has been selected to maintain pilE expression, consistent with the essential roles of Tfp in colonisation and pathogenesis.</p