4 research outputs found
Discovering the 60 years old secret: identification of the World War II mass grave victims from the island of Daksa near Dubrovnik, Croatia
Aim To describe the organization, field work, forensic anthropological
examination, and DNA analysis conducted to
identify the victims from a World War II mass grave found
on the Dalmatian island of Daksa near Dubrovnik (Croatia)
in 2009.
Methods Excavation of the site was performed according
to standard archeological procedures. Basic anthropological
examination was made to determine the minimum
number of victims, sex, age at death, and height. The bones
with pathological and traumatic changes were identified.
DNA was extracted from powdered bones and relativesā
blood samples. Y-chromosome and autosomal short tandem
repeats (STR) were used to establish the relationship
of the remains with the putative family members.
Results The remains were found to belong to at least 53
distinctive victims. All were male, mostly with gunshot
wounds to the head. DNA analysis and cross-matching of
the samples with relatives resulted in 14 positive identifications
using the Y-chromosomal STRs and 4 positive identifications
using the autosomal STRs.
Conclusions This study showed that even in cases of more
than 50-year-old, highly degraded human remains from
mass graves, Y-chromosomal and autosomal STRs analysis
can contribute to identification of the victims
Skeletal Remains from Late Roman Period: Ā»As Old as Diocletian\u27s PalaceĀ«
In 2000, human skeletal remains were discovered in Split (Croatia). As archaeologists confirmed, it was an ancient skeleton accompanied by ceramics and bracelet characteristic for late Roman period whose possible violent death was excluded. The bone sample was radiocarbon dated by AMS to 1750 years. DNA was successfully extracted from the bone sample and subsequently typed using mt DNA and STR systems. The metal content was determined by atomic absorption spectrometry (AAS) in flame mode. Mercury concentration was determined by direct consecutive measures taken with a mercury analyzer. According to our results, we consider that the bones could belong to the one of the last citizens of the Diocletianās Palace
Y-chromosomal Short Tandem Repeat Haplotypes in Southern Croatian Male Population Defined by 17 Loci
Cilj Odrediti Y-kromosomsku genetsku strukturu na uzorku muŔkaraca iz južne Hrvatske.
Postupci Uzorci krvi prikupljeni su od 166 zdravih muÅ”karaca koji nisu rodbinski povezani na Zavodu za Sudsku Medicinu i u Biokemijskom laboratoriju KliniÄke bolnice Split u razdoblju izmeÄu 2004. i 2007. Genomska DNA je dobivena pomoÄu standardnih postupaka. Sedamnaest Y-kromosomskih kratkih udvojenih ponovljenih (Y-STR) polimorfnih lokusa (DYS456, DYS389I, DYS390, DYS389II, DYS458, DYS19, DYS385, DYS393, DYS391, DYS439, DYS635, DYS392, GATAH4, DYS437, DYS438, and DYS448) analizirani su pomoÄu sustava AmpFlSTR Yfiler Polymerase Chain Reaction Amplification Kit.
Rezultati PronaÅ”li smo 152 razliÄita haplotipa. Ukupna raznolikost haplotipova bila je 0,997289, a pronaÄen je i and 141 jedinstveni haplotip (84,49%). NajÄeÅ”Äi haplotip je bio zajedniÄki samo Äetvorici muÅ”karaca. Raznolikost lokusa je bila izmeÄu 0,21292 kod lokusa DYS392 i 0,75546 kod lokusa DYS439.
ZakljuÄak Y-kromosomska graÄa muÅ”karaca iz južne Hrvatske je vrlo raznolika. Kombinacija Y-kromosomskih 17 STR lokusa može se koristiti za identifikaciju i utvrÄivanje roditeljstva u muÅ”koj populaciji južne Hrvatske.Aim To define the Y-chromosome genetic structure in a sample of
men from southern Croatia.
Methods Blood samples were collected from 166 unrelated healthy
men from southern Croatia at the Department of Forensic Medicine
and Biochemical Laboratory of University Hospital Split between
2004 and 2007. Genomic DNA was extracted using the standard
procedures. Seventeen Y-chromosome short tandem repeat (YSTR)
polymorphic loci (DYS456, DYS389I, DYS390, DYS389II,
DYS458, DYS19, DYS385, DYS393, DYS391, DYS439, DYS635,
DYS392, GATAH4, DYS437, DYS438, and DYS448) were analyzed
using AmpFlSTR Yfiler Polymerase Chain Reaction Amplification
Kit.
Results We observed 152 different haplotypes. Total haplotype
diversity was 0.997289 and 141 haplotypes (84.49%) were unique.
The most common haplotype was shared by only 4 men in the study
sample. The locus diversity ranged between 0.21292 for DYS392
and 0.75546 for DYS439 locus.
Conclusion The Y-chromosome structure in men from southern
Croatia is very diverse. Combination of Y chromosome 17 STR loci
may be used as a powerful tool for individual identification and parentage
analysis in the southern Croatian male population