Staphylococcal Infection and Infertility

Staphylococcus sp. is not only a commensal bacterium but also a major human pathogen that causes a wide range of clinical infections, such as skin and soft tissue infection, pleuropulmonary and osteoarticular infection, and endocarditis as well as life-threatening systemic infections. More evidence is currently emerging to show that Staphylococcus, particularly Staphylococcus aureus, can colonize the reproductive systems and affect their structure and function. Staphylococcal infection has become one of the most common causes of infertility in both males and females. This chapter focuses on the epidemiology, pathophysiology, clinical manifestations, and treatment of staphylococcal infection and infertility

Water-lubricated transport of high-viscosity oil in horizontal pipes: the water holdup and pressure gradient

This paper has investigated the water holdup and the pressure gradient of water-lubricated transport of high-viscosity oil flow in horizontal pipes. Experimental results on the water holdup and the pressure gradient of water-lubricated high-viscosity oil two-phase flow in a horizontal 1 in. pipe were discussed. Models for the prediction of the water holdup and/or the pressure gradient of core flow or water-lubricated flow were reviewed and evaluated. It was found that the water holdup of the water-lubricated flow is not only closely related to the input water volume fraction but also the degree of the oil phase eccentricity which is attributed to the oil phase Froude number. This can explain the inconsistency of the experimental results with regard to the relationship between the water holdup and the input water volume fraction in the literature. The applicability of the existing empirical or mechanistic models of water-lubricated high-viscosity oil flow were discussed and demonstrated. A modified correlation to the water holdup correlation of Arney et al. (1993) which was shown to be exclusively applicable for concentric core flow was introduced for stable water-lubricated flow, including both concentric and eccentric core flows. This correlation was evaluated and a fair applicability was shown. The accuracy of different models for the prediction of the pressure gradient of water-lubricated transport of high-viscosity oil was demonstrated to be not high in general. This is closely associated with the difficulty in accurately accounting for the influence of oil fouling on the pressure gradient

Jinhong decoction protects sepsis-associated acute lung injury by reducing intestinal bacterial translocation and improving gut microbial homeostasis

Background: Currently no specific treatments are available for sepsis and the associated syndromes including acute lung injury (ALI). Jinhong Decoction (JHD) is a traditional Chinese prescription, and it has been applied clinically as an efficient and safe treatment for sepsis, but the underlying mechanism remains unknown. The aim of the study was to explore the potential mechanisms of JHD ameliorating sepsis and concurrent ALI.Methods: The cecum ligation puncture (CLP)- induced murine sepsis model was established for determining the efficacy of JHD protecting CLP and ALI. The role of gut microbiota involved in the efficacy of JHD was evaluated by 16S rRNA sequencing and fecal microbiota transplantation (FMT). Translocation of intestinal Escherichia coli (E. coli) to lungs after CLP was verified by qPCR and in vivo-imaging. Intestinal permeability was analyzed by detecting FITC-dextran leakness. Junction proteins were evaluated by Western blotting and immunofluorescence.Results: JHD treatment remarkably increased survival rate of septic mice and alleviated sepsis-associated lung inflammation and injury. FMT suggested that the protective role for JHD was mediated through the regulation of gut microbiota. We further revealed that JHD administration partially restored the diversity and configuration of microbiome that was distorted by CLP operation. Of interest, the intestinal bacteria, E. coli particularly, was found to translocate into the lungs upon CLP via disrupting the intestinal mucosal barrier, leading to the inflammatory response and tissue damage in lungs. JHD impeded the migration and hence lung accumulation of intestinal E. coli, and thereby prevented severe ALI associated with sepsis. This effect is causatively related with the ability of JHD to restore intestinal barrier by up-regulating tight junctions.Conclusion: Our study unveils a mechanism whereby the migration of gut bacteria leads to sepsis-associated ALI, and we demonstrate the potential of JHD as an effective strategy to block this bacterial migration for treating sepsis and the associated immunopathology in the distal organs

An automated framework of inner segment/outer segment defect detection for retinal SD-OCT images

The integrity of inner segment/outer segment (IS/OS) has high correlation with lower visual acuity in patients suffering from blunt trauma. An automated 3D IS/OS defect detection method based on the SD-OCT images was proposed. First, 11 surfaces were automatically segmented using the multiscale 3D graph-search approach. Second, the sub-volumes between surface 7 and 8 containing IS/OS region around the fovea (diameter of mm) were extracted and flattened based on the segmented retinal pigment epithelium layer. Third, 5 kinds of texture based features were extracted for each voxel. A KNN classifier was trained and each voxel was classified as disrupted or nondisrupted and the responding defect volume was calculated. The proposed method was trained and tested on 9 eyes from 9 trauma subjects using the leave-one-out cross validation method. The preliminary results demonstrated the feasibility and efficiency of the proposed method

Identification of novel driver mutations of the discoidin domain receptor 2 (DDR2) gene in squamous cell lung cancer of Chinese patients

BACKGROUND: Although many of the recently approved genomically targeted therapies have improved outcomes for patients in non–small-cell lung cancer (NSCLC) with lung adenocarcinoma, little is known about the genomic alterations that drive lung squamous cell cancer (SCC) and development of effective targeted therapies in lung SCC is a promising area to be further investigated. Discoidin domain receptor 2 (DDR2), is a novel receptor tyrosine kinases that respond to several collagens and involved in tissue repair, primary and metastatic cancer progression. METHODS: Expression of DDR2 mRNA was analyzed in 54 lung SCC tissues by qRT-PCR. Over-expression approaches were used to investigate the biological functions of DDR2 and its’ mutations in lung SCC cells. Conventional Sanger sequencing was used to investigate the mutations of DDR2 gene in 86 samples. The effect of DDR2 and its’ mutations on proliferation was evaluated by MTT and colony formation assays; cell migration and invasion was evaluated by trasnwell assays. Lung SCC cells stably transfected with pEGFP-DDR2 WT, pEGFP-DDR2-S131C or empty vector were injection into nude mice to study the effect of DDR2 and its’ mutation on tumorigenesis in vivo. Protein and mRNA expression levels of E-cadherin and MMP2 were determined by qRT-PCR and western blot analysis. Differences between groups were tested for significance using Student’s t-test (two-tailed). RESULTS: In this study, we found that DDR2 mRNA levels were significantly decreased in 54 lung SCC tissues compared with normal lung tissues. Moreover, there were 3 novel DDR2 mutations (G531V, S131C, T681I) in 4 patients and provide the mutation rate of 4.6% in the 86 patients with lung SCC. The mutation of S131C in DDR2 could promote lung SCC cells proliferation, migration and invasion via inducing MMP-2, but reducing E-cadherin expression. CONCLUSIONS: These data indicated that the novel DDR2 mutation may contribute to the development and progression of lung SCC and this effect may be associated with increased proliferation and invasiveness, at least in part, via regulating E-cadherin expression