PDANet: Pyramid Density-aware Attention Net for Accurate Crowd Counting

Crowd counting, i.e., estimating the number of people in a crowded area, has attracted much interest in the research community. Although many attempts have been reported, crowd counting remains an open real-world problem due to the vast scale variations in crowd density within the interested area, and severe occlusion among the crowd. In this paper, we propose a novel Pyramid Density-Aware Attention-based network, abbreviated as PDANet, that leverages the attention, pyramid scale feature and two branch decoder modules for density-aware crowd counting. The PDANet utilizes these modules to extract different scale features, focus on the relevant information, and suppress the misleading ones. We also address the variation of crowdedness levels among different images with an exclusive Density-Aware Decoder (DAD). For this purpose, a classifier evaluates the density level of the input features and then passes them to the corresponding high and low crowded DAD modules. Finally, we generate an overall density map by considering the summation of low and high crowded density maps as spatial attention. Meanwhile, we employ two losses to create a precise density map for the input scene. Extensive evaluations conducted on the challenging benchmark datasets well demonstrate the superior performance of the proposed PDANet in terms of the accuracy of counting and generated density maps over the well-known state of the arts

Metabolic reconfiguration enables synthetic reductive metabolism in yeast

Cell proliferation requires the integration of catabolic processes to provide energy, redox power and biosynthetic precursors. Here we show how the combination of rational design, metabolic rewiring and recombinant expression enables the establishment of a decarboxylation cycle in the yeast cytoplasm. This metabolic cycle can support growth by supplying energy and increased provision of NADPH or NADH in the cytosol, which can support the production of highly reduced chemicals such as glycerol, succinate and free fatty acids. With this approach, free fatty acid yield reached 40% of theoretical yield, which is the highest yield reported for Saccharomyces cerevisiae to our knowledge. This study reports the implementation of a synthetic decarboxylation cycle in the yeast cytosol, and its application in achieving high yields of valuable chemicals in cell factories. Our study also shows that, despite extensive regulation of catabolism in yeast, it is possible to rewire the energy metabolism, illustrating the power of biodesign

Mahalanobis Distance Map Approach for Anomaly Detection

Web servers and web-based applications are commonly used as attack targets. The main issues are how to prevent unauthorised access and to protect web servers from the attack. Intrusion Detection Systems (IDSs) are widely used security tools to detect cyber-attacks and malicious activities in computer systems and networks. In this paper, we focus on the detection of various web-based attacks using Geometrical Structure Anomaly Detection (GSAD) model and we also propose a novel algorithm for the selection of most discriminating features to improve the computational complexity of payload-based GSAD model. Linear Discriminant method (LDA) is used for the feature reduction and classification of the incoming network traffic. GSAD model is based on a pattern recognition technique used in image processing. It analyses the correlations between various payload features and uses Mahalanobis Distance Map (MDM) to calculate the difference between normal and abnormal network traffic. We focus on the detection of generic attacks, shell code attacks, polymorphic attacks and polymorphic blending attacks. We evaluate accuracy of GSAD model experimentally on the real-world attacks dataset created at Georgia Institute of Technology. We conducted preliminary experiments on the DARPA 99 dataset to evaluate the accuracy of feature reduction

Ginkgo Biloba Extract EGB761 Protects against Aging-Associated Diastolic Dysfunction in Cardiomyocytes of D-Galactose-Induced Aging Rat

The aim of the present study was to make use of the artificially induced aging model cardiomyocytes to further investigate potential anti-aging-associated cellular diastolic dysfunction effects of EGB761 and explore underlying molecular mechanisms. Cultured rat primary cardiomyocytes were treated with either D-galactose or D-galactose combined with EGB761 for 48 h. After treatment, the percentage of cells positive for SA-β-gal, AGEs production, cardiac sarcoplasmic reticulum calcium pump (SERCA) activity, the myocardial sarcoplasmic reticulum calcium uptake, and relative protein levels were measured. Our results demonstrated that in vitro stimulation with D-galactose induced AGEs production. The addition of EGB761 significantly decreased the number of cells positive for SA-β-gal. Furthermore, decreased diastolic [Ca2+]i, curtailment of the time from the maximum concentration of Ca2+ to the baseline level and increased reuptake of Ca2+ stores in the SR were also observed. In addition, the level of p-Ser16-PLN protein as well as SERCA was markedly increased. The study indicated that EGb761 alleviates formation of AGEs products on SERCA2a in order to mitigate myocardial stiffness on one hand; on other hand, improve SERCA2a function through increase the amount of Ser16 sites PLN phosphorylation, which two hands finally led to ameliorate diastolic dysfunction of aging cardiomyocytes

DropKey

In this paper, we focus on analyzing and improving the dropout technique for self-attention layers of Vision Transformer, which is important while surprisingly ignored by prior works. In particular, we conduct researches on three core questions: First, what to drop in self-attention layers? Different from dropping attention weights in literature, we propose to move dropout operations forward ahead of attention matrix calculation and set the Key as the dropout unit, yielding a novel dropout-before-softmax scheme. We theoretically verify that this scheme helps keep both regularization and probability features of attention weights, alleviating the overfittings problem to specific patterns and enhancing the model to globally capture vital information; Second, how to schedule the drop ratio in consecutive layers? In contrast to exploit a constant drop ratio for all layers, we present a new decreasing schedule that gradually decreases the drop ratio along the stack of self-attention layers. We experimentally validate the proposed schedule can avoid overfittings in low-level features and missing in high-level semantics, thus improving the robustness and stableness of model training; Third, whether need to perform structured dropout operation as CNN? We attempt patch-based block-version of dropout operation and find that this useful trick for CNN is not essential for ViT. Given exploration on the above three questions, we present the novel DropKey method that regards Key as the drop unit and exploits decreasing schedule for drop ratio, improving ViTs in a general way. Comprehensive experiments demonstrate the effectiveness of DropKey for various ViT architectures, e.g. T2T and VOLO, as well as for various vision tasks, e.g., image classification, object detection, human-object interaction detection and human body shape recovery.Comment: Accepted by CVPR202