Study on vibration characteristics of rolling mill based on vibration absorber

The vertical vibration often occurs during the rolling production, which has an influence on the accuracy of rolling mill. In order to effectively suppress the vertical vibration of the rolling equipment, the rolling mill model with vibration absorber device was established. Based on the main resonance singularity of the rolling mill system, the best combination of opening parameters was obtained. The best combination of opening parameters helps the rolling mill system work in a stable area. Finally, the effects of different vibration absorber parameters on the vibration characteristics of the rolling mill system were analyzed. Results show that the vibration absorber device can effectively improve the stability of the rolling mill system

A Pilot Study on Dialogue-Level Dependency Parsing for Chinese

Dialogue-level dependency parsing has received insufficient attention, especially for Chinese. To this end, we draw on ideas from syntactic dependency and rhetorical structure theory (RST), developing a high-quality human-annotated corpus, which contains 850 dialogues and 199,803 dependencies. Considering that such tasks suffer from high annotation costs, we investigate zero-shot and few-shot scenarios. Based on an existing syntactic treebank, we adopt a signal-based method to transform seen syntactic dependencies into unseen ones between elementary discourse units (EDUs), where the signals are detected by masked language modeling. Besides, we apply single-view and multi-view data selection to access reliable pseudo-labeled instances. Experimental results show the effectiveness of these baselines. Moreover, we discuss several crucial points about our dataset and approach.Comment: Accepted by Findings of ACL 2023 (Camera-ready version

Christensenella minuta interacts with multiple gut bacteria

IntroductionGut microbes form complex networks that significantly influence host health and disease treatment. Interventions with the probiotic bacteria on the gut microbiota have been demonstrated to improve host well-being. As a representative of next-generation probiotics, Christensenella minuta (C. minuta) plays a critical role in regulating energy balance and metabolic homeostasis in human bodies, showing potential in treating metabolic disorders and reducing inflammation. However, interactions of C. minuta with the members of the networked gut microbiota have rarely been explored.MethodsIn this study, we investigated the impact of C. minuta on fecal microbiota via metagenomic sequencing, focusing on retrieving bacterial strains and coculture assays of C. minuta with associated microbial partners.ResultsOur results showed that C. minuta intervention significantly reduced the diversity of fecal microorganisms, but specifically enhanced some groups of bacteria, such as Lactobacillaceae. C. minuta selectively enriched bacterial pathways that compensated for its metabolic defects on vitamin B1, B12, serine, and glutamate synthesis. Meanwhile, C. minuta cross-feeds Faecalibacterium prausnitzii and other bacteria via the production of arginine, branched-chain amino acids, fumaric acids and short-chain fatty acids (SCFAs), such as acetic. Both metagenomic data analysis and culture experiments revealed that C. minuta negatively correlated with Klebsiella pneumoniae and 14 other bacterial taxa, while positively correlated with F. prausnitzii. Our results advance our comprehension of C. minuta’s in modulating the gut microbial network.ConclusionsC. minuta disrupts the composition of the fecal microbiota. This disturbance is manifested through cross-feeding, nutritional competition, and supplementation of its own metabolic deficiencies, resulting in the specific enrichment or inhibition of the growth of certain bacteria. This study will shed light on the application of C. minuta as a probiotic for effective interventions on gut microbiomes and improvement of host health

Femtosecond Pulse Temporal Overlap Estimation and Adjustment in SSFS-Based CARS System

This work is licensed under a Creative Commons Attribution 4.0 International License.We present and verify a residual-pump-based temporal overlap estimation method in soliton self-frequency shift-based coherent anti-Stokes Raman scattering system. The residual pump light, output by a highly nonlinear photonic crystal fiber, acts as a crucial link between the pump and Stokes pulses during the temporal overlap estimation. The wavelength-dependent optical delay is estimated to be 0.141 ps/nm when the Stokes wavelength is 900 nm ~1050 nm according to the temporal overlap estimation method. The actual measurement result is 0.138 ps/nm based on the nonresonant signal from a microscope slide, which is very close to the estimated result. In addition, the Raman resonant signals of liquid cyclohexane at 2853 cm -1 , 2923 cm -1 and 2938 cm -1 have also been successfully detected at the predicted optical delays 427.27 ps and 428.17 ps

From Rayleigh-B\'enard convection to porous-media convection: how porosity affects heat transfer and flow structure

We perform a numerical study of the heat transfer and flow structure of Rayleigh-B\'enard (RB) convection in (in most cases regular) porous media, which are comprised of circular, solid obstacles located on a square lattice. This study is focused on the role of porosity $\phi$ in the flow properties during the transition process from the traditional RB convection with $\phi=1$ (so no obstacles included) to Darcy-type porous-media convection with $\phi$ approaching 0. Simulations are carried out in a cell with unity aspect ratio, for the Rayleigh number $Ra$ from $10^5$ to $10^{10}$ and varying porosities $\phi$, at a fixed Prandtl number $Pr=4.3$, and we restrict ourselves to the two dimensional case. For fixed $Ra$, the Nusselt number $Nu$ is found to vary non-monotonously as a function of $\phi$; namely, with decreasing $\phi$, it first increases, before it decreases for $\phi$ approaching 0. The non-monotonous behaviour of $Nu(\phi)$ originates from two competing effects of the porous structure on the heat transfer. On the one hand, the flow coherence is enhanced in the porous media, which is beneficial for the heat transfer. On the other hand, the convection is slowed down by the enhanced resistance due to the porous structure, leading to heat transfer reduction. For fixed $\phi$, depending on $Ra$, two different heat transfer regimes are identified, with different effective power-law behaviours of $Nu$ vs $Ra$, namely, a steep one for low $Ra$ when viscosity dominates, and the standard classical one for large $Ra$. The scaling crossover occurs when the thermal boundary layer thickness and the pore scale are comparable. The influences of the porous structure on the temperature and velocity fluctuations, convective heat flux, and energy dissipation rates are analysed, further demonstrating the competing effects of the porous structure to enhance or reduce the heat transfer

Intracellular Delivery of Recombinant alpha B-crystallin into Neonatal Rat Cardiomyocytes has a Protective Effect on the Cells

In order to deliver alpha B-crystallin (alpha B-C) into cardiomyocytes and study its cellular protection, the full-length cDNA fragment encoding human alpha B-C was cloned into the bacterial expression vector pGEX-MTS containing the base sequence of membrane-translocating sequence (MTS) which mediates intracellular delivery of peptides and expressed as a fusion protein coupled to glutathione S-transferase (GST).After glutathione affinity chromatography and cleaved from GST by factor Xa, the recombinant MTS- alpha B-C was separated from GST and factor Xa by anion exchange chromatography. Recombinant MTS- alpha B-C was characterized by SDS-PAGE and Western immunoblot analysis. The purified MTS- alpha B-C migrated on SDS-PAGE as a single band to an apparent molecular weight (Mr.23kD) that corresponded to total native alpha B-C plus MTS, and was recognized on Western immunoblot by anti-human alpha B-crystallin antibody. MTS- alpha B-C displayed chaperone-like function in an ATP-containing buffer at 37? by disaggregating the denatured and aggregated actin induced by hydrogen peroxide (H2O2 )treatment. It was observed under fluorescence microscope that FITC-labeled MTS- alpha B-C had gone into neonatal rat cardiomyocytes by MTS mediation after the cells were incubated with it for 6 hours while FITC-labeled alpha B-C and bovine serum albumin had not gone into the cells. Recombinant MTS- alpha B-C is not cytotoxic, and MTS- alpha B-C-treated cells displayed increased H2O2-tolerance compared with non-treated cells