Plasmid-Linked Maltose Utilization in Lactobacillus spp. Isolated from Meat

Five strains of Lactobacillus plantarum and four strains of Lactobacillus species isolated from fresh meat were examined for the presence of plasmid DNA. All strains examined contained between one and five plasmids ranging in molecular mass from 1.3 to 51.6 (Mdal). Plasmid-curing experiments suggest that maltose utilization is associated with a 51 Mdal plasmid in Lactobacillus sp. DB29 and 42 Mdal plasmids in Lactobacillus spp. DB27, DB28, DB31. Southern blot DNA-DNA hybridization showed homology between the maltose plasmid from Lactobacillus sp. DB29 and several plasmids from the other Lactobacillus spp

Impact of Sucrose Addition on the Physiochemical Properties and Volatile Compounds of “Shuangyou” Red Wines

“Shuangyou,” a Vitis amurensis Rupr. variety, is widely cultivated in northeastern and western China. Its berries have high acidity and low sugar content. In this study, different proportions of sucrose were added to the must samples during fermentation to investigate the effect of sugar on the physicochemical properties and volatile compounds of “Shuangyou” wines. The addition of sucrose significantly improved yeast growth and alcohol production, altered the color qualities, and slightly decreased titratable acidity during fermentation. The highest tested proportion of added sucrose resulted in the highest maximum yeast counts and final ethanol concentrations. Moreover, 37 volatile compounds (esters, alcohols, fatty acids, ketones, and aldehydes) were identified and quantified by solid-phase microextraction with gas chromatography-mass spectrometry. The concentrations of these compounds were correlated with the addition of sucrose. Furthermore, the addition of 100 g/L sucrose was sufficient for improving the concentrations of the aromatic compounds. The increase in ester, alcohol, and fatty acid concentration led to a positive OAVs impact (odor activity value > 1) at the end of fermentation

临床压疮管理简易方法探究

To guarantee the fulfilling of nursing for pressure sores patients efficiently and normatively, we established "The quality evaluation standard of pressure score management", in which the content were comprehensive and the standard were definite.It is widely applied for clinical supervision, unified examination method, to guide clinical work, narrow the difference of evaluation, popularize the knowledge of pressure scores, improve the ability of would disposal, reported compliance rates of high risk of pressure scores increased as well as the rate of cure and nursing care of patients with pressure scores. Nursing care of patients with pressure sore was from passive to active, measures are effectively implemented, the management level of the skin was improved so that it turns out to be a simple and effective method. 为保证压疮护理各项措施的有效、规范落实，制定了《压疮管理质量评价标准》，其涵盖内容全面，工作标准明确。并依此进行临床督导，统一检查方法，指引临床工作，缩小评估差异，普及压疮知识，提高了创面处置能力，高危压疮上报符合率及压疮治愈好转率提高，压疮护理由被动变主动，措施得到有效落实，提高了皮肤管理水平，方法简单有效

trans-Tetra­kis(1-allyl-1H-imidazole-κN 3)bis­(thio­cyanato-κN)nickel(II)

The structure of the title compound, [Ni(NCS)2(C6H8N2)4], consists of isolated mol­ecules of [Ni(NCS)2(Aim)4] (Aim = 1-allyl­imidazole), which contain a distorted octa­hedral NiN6 chromophore. The NCS− anions are trans and four N atoms from the 1-allyl­imidazole ligands define the equatorial plane. The mean Mn—N(Aim) and Mn—N(NCS) distances are 2.105 (2) and 2.098 (2) Å, respectively. Weak C—H⋯N inter­actions contribute to the crystal packing stability

Early Functional and Cognitive Declines Measured by Auditory-Evoked Cortical Potentials in Mice With Alzheimer’s Disease

Alzheimer’s disease (AD) is characterized by a progressive loss of memory and cognitive decline. However, the assessment of AD-associated functional and cognitive changes is still a big challenge. Auditory-evoked cortical potential (AECP) is an event-related potential reflecting not only neural activation in the auditory cortex (AC) but also cognitive activity in the brain. In this study, we used the subdermal needle electrodes with the same electrode setting as the auditory brainstem response (ABR) recording and recorded AECP in normal aging CBA/CaJ mice and APP/PS1 AD mice. AECP in mice usually appeared as three positive peaks, i.e., P1, P2, and P3, and three corresponding negative peaks, i.e., N1, N2, and N3. In normal aging CBA mice, the early sensory peaks P1, N1, and P2 were reduced as age increased, whereas the later cognitive peaks N2, P3, and N3 were increased or had no changes with aging. Moreover, the latency of the P1 peak was increased as age increased, although the latencies of later peaks had a significant reduction with aging. In AD mice, peak P1 was significantly reduced in comparison with wild-type (WT) littermates at young ages, proceeding AD phenotype presentation. In particular, the later cognitive peak P3 was diminished after 3 months old, different from the normal aging effect. However, the latencies of AECP peaks in AD mice generally had no significant delay or changes with aging. Finally, consistent with AECP changes, the accumulation of amyloid precursor protein (APP) at the AC was visible in AD mice as early as 2 months old. These data suggest that AECP could serve as an early, non-invasive, and objective biomarker for detecting AD and AD-related dementia (ADRD)

COCA: Classifier-Oriented Calibration for Source-Free Universal Domain Adaptation via Textual Prototype

Universal Domain Adaptation (UniDA) aims to distinguish common and private classes between the source and target domains where domain shift exists. Recently, due to more stringent data restrictions, researchers have introduced Source-Free UniDA (SF-UniDA) in more realistic scenarios. SF-UniDA methods eliminate the need for direct access to source samples when performing adaptation to the target domain. However, existing SF-UniDA methods still require an extensive quantity of labeled source samples to train a source model, resulting in significant labeling costs. To tackle this issue, we present a novel Classifier-Oriented Calibration (COCA) method. This method, which leverages textual prototypes, is formulated for the source model based on few-shot learning. Specifically, we propose studying few-shot learning, usually explored for closed-set scenarios, to identify common and domain-private classes despite a significant domain shift between source and target domains. Essentially, we present a novel paradigm based on the vision-language model to learn SF-UniDA and hugely reduce the labeling costs on the source domain. Experimental results demonstrate that our approach outperforms state-of-the-art UniDA and SF-UniDA models

(E)-N′-[(2-Hydroxynaphthalen-1-yl)methylidene]nicotinohydrazide

In the mol­ecule of the title compound, C17H13N3O2, the naphthyl ring system and the pyridine ring form a dihedral angle of 12.2 (3)°. An intra­molecular O—H⋯N hydrogen bond generates a six-membered ring with an S(6) ring motif. This also contributes to the relative overall near planarity of the mol­ecule [r.m.s. deviation of all 22 non-H atoms = 0.107 (5) Å]. In the crystal, mol­ecules are linked through inter­molecular N—H⋯N hydrogen bonds, forming chains along the a axis

(Diphenyl­phosphor­yl)(2-nitro­phen­yl)methanol

In the title compound, C19H16NO4P, the dihedral angle between the mean planes of the phenyl rings bonded to the P atom is 75.4 (1)°. In the crystal, mol­ecules are linked into chains running along the a axis by inter­molecular O—H⋯O hydrogen bonds. Mol­ecules are further connected into a three-dimensional array by weak C—H⋯O hydrogen bonds

(E)-Methyl 2-chloro-4-dicyclo­hexyl­amino-4-oxobut-2-enoate

In the title compound, C17H26ClNO3, both cyclo­hexyl rings have chair conformations. In the crystal, mol­ecules are linked by weak inter­molecular C—H⋯O hydrogen bonds

MiRNA-145 increases therapeutic sensibility to gemcitabine treatment of pancreatic adenocarcinoma cells.

Pancreatic adenocarcinoma is one of the most leading causes of cancer-related deaths worldwide. Although recent advances provide various treatment options, pancreatic adenocarcinoma has poor prognosis due to its late diagnosis and ineffective therapeutic multimodality. Gemcitabine is the effective first-line drug in pancreatic adenocarcinoma treatment. However, gemcitabine chemoresistance of pancreatic adenocarcinoma cells has been a major obstacle for limiting its treatment effect. Our study found that p70S6K1 plays an important role in gemcitabine chemoresistence. MiR-145 is a tumor suppressor which directly targets p70S6K1 for inhibiting its expression in pancreatic adenocarcinoma, providing new therapeutic scheme. Our findings revealed a new mechanism underlying gemcitabine chemoresistance in pancreatic adenocarcinoma cells